Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator

EMBO Reports

Volumn 5, Issue 12, 2004, Pages 1176-1180

  Ponsioen, Bas ^a   Zhao, Jun ^b   Riedl, Jurgen ^b   Zwartkruis, Fried ^b   van der Krogt, Gerard ^a   Zaccolo, Manuela ^c   Moolenaar, Wouter H ^a   Bos, Johannes L ^b   Jalink, Kees ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^c VENETIAN INSTITUTE OF MOLECULAR MEDICINE   (Italy)

Author keywords

cAMP; Epac; FRET; PKA

Indexed keywords

CYAN FLUORESCENT PROTEIN; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; GUANINE NUCLEOTIDE EXCHANGE FACTOR; HYBRID PROTEIN; POLYPEPTIDE; PROTEIN EPAC1; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; BIOSENSOR; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME SUBUNIT; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE CONSTRUCT; IN VIVO STUDY; MAMMAL CELL; MOLECULAR PROBE; MONITORING; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; SENSITIVITY ANALYSIS; SIGNAL NOISE RATIO; STATISTICAL SIGNIFICANCE;

ANIMALS; BIOLOGICAL MARKERS; BIOSENSING TECHNIQUES; CYCLIC AMP; CYCLIC AMP-DEPENDENT PROTEIN KINASES; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENES, REPORTER; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; PROTEIN CONFORMATION;

MAMMALIA;

EID: 12144282761     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.embor.7400290     Document Type: Article

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