Synthetic chemokines directly labeled with a fluorescent dye as tools for studying chemokine and chemokine receptor interactions

European Cytokine Network

Volumn 17, Issue 1, 2006, Pages 49-59

  Strong, Andrew E ^a   Thierry, Anne Christine ^b   Cousin, Pascal ^c   Moulon, Corinne ^d   Demotz, Stéphane ^e  

^a LONZA AG   (Switzerland)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^c UNIVERSITY OF LAUSANNE   (Switzerland)

^d DEBIOPHARM SA   (Switzerland)

^e Apoxis SA   (Switzerland)

Author keywords

Alexa Fluor 647; Antagonist; Flow cytometry; Fluorescence; High throughput screening

Indexed keywords

ALPHA CHEMOKINE; BETA CHEMOKINE; CHEMOKINE; CHEMOKINE RECEPTOR; CHEMOKINE RECEPTOR CXCR4; FLUORESCENT DYE; LIGAND; MACROPHAGE INFLAMMATORY PROTEIN 3BETA; STROMAL CELL DERIVED FACTOR 1;

ARTICLE; CELL LINE; CHEMISTRY; DRUG POTENTIATION; HUMAN; METABOLISM; NEUTROPHIL CHEMOTAXIS; SYNTHESIS;

CELL LINE; CHEMOKINES; CHEMOKINES, CC; CHEMOKINES, CXC; CHEMOTAXIS, LEUKOCYTE; FLUORESCENT DYES; HUMANS; LIGANDS; RECEPTORS, CHEMOKINE; RECEPTORS, CXCR4;

EID: 33746059073     PISSN: 11485493     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Rossi D, Zlotnik A. The biology of chemokines and their receptors. Annu Rev Immunol 2000; 18: 217.
2. Sallusto F, Mackay CR, Lanzavecchia A. The role of chemokine receptors in primary, effector, and memory immune responses. Annu Rev Immunol 2000; 18: 593.
3. Moser B, Wolf M, Walz A, Loetscher P. Chemokines: multiple levels of leukocyte migration control. Trends Immunol 2004; 25: 75.
4. Shin WS, Szuba A, Rockson SG. The role of chemokines in human cardiovascular pathology: enhanced biological insights. Atherosclerosis 2002; 160: 91.
5. Zimmermann N, Hershey GK, Foster PS, Rothenberg ME. Chemokines in asthma: cooperative interaction between chemokines and IL-13. J Allergy Clin Immunol 2003; 111: 227.
6. D'Ambrosio D, Panina-Bordignon P, Sinigaglia F. Chemokine receptors in inflammation: an overview. J Immunol Methods 2003; 273: 3.
7. Szekanecz Z, Kim J, Koch AE. Chemokines and chemokine receptors in rheumatoid arthritis. Semin Immunol 2003; 15: 15.
8. Arimilli S, Ferlin W, Solvason N, Deshpande S, Howard M, Mocci S. Chemokines in autoimmune diseases. Immunol Rev 2000; 177: 43.
9. Meanwell NA, Kadow JF. Inhibitors of the entry of HIV into host cells. Curr Opin Drug Discov Devel 2003; 6: 451.
10. el-Sawy T, Fahmy NM, Fairchild RL. Chemokines: directing leukocyte infiltration into allografts. Curr Opin Immunol 2002; 14: 562.
11. Homey B, Muller A, Zlotnik A. Chemokines: agents for the immunotherapy of cancer? Nat Rev Immunol 2002; 2: 175.
12. Horuk R. Chemokine receptors. Cyt Growth Fact Rev 2001; 12: 313.
13. Fernandez EJ, Lolis E. Structure, function, and inhibition of chemokines. Annu Rev Pharmacol Toxicol 2002; 42: 469.
14. Proudfoot AEI. Chemokine receptors: Multifaceted therapeutic targets. Nat Rev Immunol 2002; 2: 106.
15. Schwarz MK, Wells TN. New therapeutics that modulate chemokine networks. Nat Rev Drug Discov 2002; 1: 347.
16. Scozzafava A, Mastrolorenzo A, Supuran CT. Non-peptidic chemokine receptors antagonists as emerging anti-HIV agents. J Enzyme Inhib Med Chem 2002; 17: 69.
17. Elices MJ. BX-471 Berlex. Curr Opin Investig Drugs 2002; 3: 865.
18. Zabel BA, Agace WW, Campbell JJ, Heath HM, Parent D, Roberts AI, Ebert EC, Kassam N, Qin S, Zovko M, LaRosa GJ, Yang LL, Soler D, Butcher EC, Ponath PD, Parker CM, Andrew DP. Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis. J Exp Med 1999; 190: 1241.
19. Gonsiorek W, Zavodny P, Hipkin RW. The study of CXCR3 and CCR7 pharmacology using [35S] GTPgammaS exchange assays in cell membranes and permeabilized peripheral blood lymphocytes. J Immunol Methods 2003; 273: 15.
20. Hatse S, Princen K, Liekens S, Vermeire K, De Clercq E, Schols D. Fluorescent CXCL12AF647 as a novel probe for nonradioactive CXCL12/CXCR4 cellular interaction studies. Cytometry 2004; 61: 178.
21. Clark-Lewis I. Synthesis of chemokines. Methods Mol Bio 2000; 138: 47.
22. Roggero MA, Meraldi V, Lopez JA, Eberl G, Romero JC, Matile H, Betschart B, Corradin G, Renggli J. The synthetic, oxidized C-terminal fragment of the Plasmodium berghei circumsporozoite protein elicits a high protective response. Eur J Immunol 2000; 30: 2679.
23. Thierry AC, Perrenoud G, Pinaud S, Bigler N, Denis B, Roggero M, Moulon C, Demotz S. Biotinylated synthetic chemokines: Their use for the development of non-radioactive whole-cell binding assays. J Biomol Screen 2003; 8: 316.
24. Loetscher P, Pellegrino A, Gong JH, Mattioli I, Loetscher M, Bardi G, Baggiolini M, Clark-Lewis I. The ligands of CXC chemokine receptor 3, I-TAC, Mig, and IP10, are natural antagonists for CCR3. J Biol Chem 2001; 276: 2986.
25. Zhao Q, Lu H, Schols D, de Clerq E, Jiang S. Development of a cell-based enzyme-linked immunosorbent assay for high-throughput screening of HIV type I entry inhibitors targeting the coreceptor CXCR4. AIDS Res Hum Retroviruses 2003; 19: 947.
26. White JR, Lee JM, Dede K, Imburgia CS, Jurewicz AJ, Chan G, Fornwald JA, Dhanak D, Christmann LT, Darcy MG, Widdowson KL, Foley JJ, Schmidt DB, Sarau HM. Identification of potent, selective non-peptide CC chemokine receptor-3 antagonist that inhibits eotaxin-, eotaxin-2-, and monocyte chemotactic protein-4-induced eosinophil migration. J Biol Chem 2000; 275: 36626.
27. Richter R, Schulz-Knappe P, John H, Forssmann WG. Post-translationally processed forms of the human chemokine HCC-1. Biochemistry 2000; 39: 10799.
28. Marcaurelle LA, Mizoue LS, Wilken J, Oldham L, Kent SB, Handel TM, Bertozzi CR. Chemical synthesis of lymphotactin: a glycosylated chemokine with a C-terminal mucin-like domain. Chemistry (Easton) 2001; 7: 1129.
29. Menten P, Wuyts A, Van Damme J. Monocyte chemotactic protein-3. Eur Cytokine Netw 2001; 12: 554.
30. Weber PJ, Bader JE, Folkers G, Beck-Sickinger AG. A fast and inexpensive method for N-terminal fluorescein-labeling of peptides. Bioorg Med Chem Lett 1998; 8: 597.
31. Haskell CA, Cleary MD, Charo IF. Unique role of the chemokine domain of fractalkine in cell capture. Kinetics of receptor dissociation correlates with cell adhesion. J Biol Chem 2000; 275: 34183.