A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells

Nature Methods

Volumn 2, Issue 3, 2005, Pages 171-176

  Hoffmann, Carsten ^a   Gaietta, Guido ^b   Bünemann, Moritz ^a   Adams, Stephen R ^b   Oberdorff Maass, Silke ^a   Behr, Björn ^a   Vilardaga, Jean Pierre ^a   Tsien, Roger Y ^b   Ellisman, Mark H ^b   Lohse, Martin J ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE A2A RECEPTOR; ADENYLATE CYCLASE; ALPHA 2A ADRENERGIC RECEPTOR; ARSENIC DERIVATIVE; CYAN FLUORESCENT PROTEIN; CYSTEINE DERIVATIVE; FLUORESCENT DYE; G PROTEIN COUPLED RECEPTOR; YELLOW FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; KINETICS; MOLECULAR MODEL; MOLECULAR SIZE; MONITORING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; SIGNAL DETECTION; ANIMAL; CELL CULTURE; CELL STRAIN COS1; CERCOPITHECUS; COMPARATIVE STUDY; EVALUATION; HELA CELL; KIDNEY; METABOLISM; METHODOLOGY; PROTEIN ANALYSIS;

ANIMALS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; COS CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; GTP-BINDING PROTEINS; HELA CELLS; HUMANS; KIDNEY; PROTEIN INTERACTION MAPPING; RECEPTOR, ADENOSINE A2A; RECEPTORS, G-PROTEIN-COUPLED;

EID: 20844434337     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth742     Document Type: Article

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