Single-molecule imaging of EGFR signalling on the surface of living cells

Nature Cell Biology

Volumn 2, Issue 3, 2000, Pages 168-172

  Sako, Yasushi ^a   Minoghchi, Shigeru ^a   Yanagida, Toshio ^b  

^a OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b Japan Science and Technology Agency   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; MONOCLONAL ANTIBODY;

ANTIBODY SPECIFICITY; ARTICLE; AUTOPHOSPHORYLATION; CELL SURFACE; COMPLEX FORMATION; DIMERIZATION; FLUORESCENCE; HUMAN; HUMAN CELL; IMAGING SYSTEM; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

ANTIBODIES, MONOCLONAL; CALCIUM; CARBOCYANINES; CARCINOMA; CELL MEMBRANE; DIMERIZATION; ENERGY TRANSFER; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYES; HUMANS; INTRACELLULAR FLUID; MICROSCOPY, FLUORESCENCE; PHOSPHORYLATION; RECEPTOR, EPIDERMAL GROWTH FACTOR; RHODAMINES; SIGNAL TRANSDUCTION; TUMOR CELLS, CULTURED;

EID: 0033779765     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/35004044     Document Type: Article

References (26)

1. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
2. Fluorescence spectroscopy of single biomolecules
3. Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy
4. Epidermal growth factor induces rapid, reversible aggregation of the purified epidermal growth factor receptor
5. Self-phosphorylation of epidermal growth factor receptor: Evidence for a model of intermolecular allosteric activation
6. Heterogeneity of epidermal growth factor binding kinetics on individual cells
7. Comparison of epidermal growth factor (EGF) receptor-receptor interactions in intact A431 cells and isolated plasma membrane
8. Single-molecule microscopy on model membranes reveals anomalous diffusion
9. An artificial lipid bilayer formed on an agarose-coated glass for simultaneous electrical and optical measurement of single ion-channels
10. Temperature-dependent lateral and transverse distribution of the epidermal growth factor receptor in A431 plasma membranes
11. Microaggregation of hormone-occupied epidermal growth factor receptors on plasma membrane preparations
12. Oligomerization of epidermal growth factor receptors on A 431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation
13. The EGF receptor is an actin-binding protein
14. Epidermal growth factor receptors associated to cytoskeletal elements of epidermoid carcinoma (A431) cells
15. Fluorescence resonance energy transfer between single fluorophores attached to a coiled-coil protein in aqueous solution
16. Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium
17. Immunodetection of the ligand-activated receptor for epidermal growth factor
18. Basolateral protein transport in streptolysin O-permeabilized MDCK cells
19. Two EGF molecules contribute additively to stabilization of the EGFR dimer
20. EGF induces increased ligand binding affinity and dimerization of soluble epidermal growth factor (EGF) receptor extracellular domain
21. Stabilization of an active dimeric form of the epidermal growth factor receptor by introduction of an inter-receptor disulfide bond
22. Bivalence of EGF-like ligands drives the ErbB signaling network
23. Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: Fence versus tether
24. Cell surface organization by the membrane skeleton
25. Cytoplasmic regulation of the movement of E-cadherin on the free cell surface as studied by optical tweezers and single particle tracking: Corralling and tethering by the membrane skeleton
26. Visualization of epidermal growth factor (EGF) receptor aggregation in plasma membranes by fluorescence resonance energy transfer. Correlation of receptor activation with aggregation