Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis

Journal of Biological Chemistry

Volumn 283, Issue 3, 2008, Pages 1525-1533

  Ye, Shixin ^a   Köhrer, Caroline ^b   Huber, Thomas ^a   Kazmi, Manija ^a   Sachdev, Pallavi ^a   Yan, Elsa C Y ^a,c   Bhagat, Aditi ^a,c   RajBhandary, Uttam L ^b   Sakmar, Thomas P ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID MUTAGENESIS; G PROTEIN-COUPLED RECEPTORS; MAMMALIAN CELLS;

BIOLOGICAL MEMBRANES; CROSSLINKING; GENE EXPRESSION; LIGANDS; MUTAGENESIS; PROTEINS; SIGNAL TRANSDUCTION;

AMINO ACIDS;

4 ACETYLPHENYLALANINE; 4 BENZOYLPHENYLALANINE; AMINO ACID; CHEMOKINE RECEPTOR CCR5; FLUORESCENT DYE; G PROTEIN COUPLED RECEPTOR; HYDRAZIDE; PHENYLALANINE DERIVATIVE; RETINA S ANTIGEN; RHODOPSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CALCIUM TRANSPORT; CELL SURFACE; CHEMICAL REACTION; CONTROLLED STUDY; FLUORESCENCE; IMMUNOBLOTTING; MAMMAL CELL; MOLECULAR PROBE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; WILD TYPE;

AMINO ACIDS; AMINOACYLATION; BACILLUS STEAROTHERMOPHILUS; BENZOPHENONES; CELL LINE; ESCHERICHIA COLI; HUMANS; LUCIFERASES; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PHENYLALANINE; RECEPTORS, CCR5; RECEPTORS, G-PROTEIN-COUPLED; RHODOPSIN; RNA, TRANSFER, TYR; TYROSINE-TRNA LIGASE;

HUMAN IMMUNODEFICIENCY VIRUS; MAMMALIA;

EID: 38349107042     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M707355200     Document Type: Article

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