Fluorescein-labeled naloxone binding to mu opioid receptors on live Chinese hamster ovary cells using confocal fluorescent microscopy

Journal of Neuroscience Methods

Volumn 97, Issue 2, 2000, Pages 123-131

  Madsen, Barry W ^a   Beglan, Carol L ^b   Spivak, Charles E ^b  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b NIDA Addiction Research Center   (United States)

Author keywords

Chinese hamster ovary cells; Confocal fluorescent microscopy; Fluorescent probe; Ligand kinetics; Live cells; Mu opioid receptor; Radioligand binding

Indexed keywords

3,4 DICHLORO N METHYL N [2 (1 PYRROLIDINYL)CYCLOHEXYL]BENZENEACETAMIDE; DEXTRO PHENYLALANYLCYSTEINYLTYROSYL DEXTRO TRYPTOPHYLORNITHYLTHREONYLPENICILLAMINYLTHREONINAMIDE 2,7 DISULFIDE; ENKEPHALIN[2,5 DEXTRO PENICILLAMINE]; FLUORESCEIN; FLUORESCENT DYE; MU OPIATE RECEPTOR; NALOXONE; RADIOLIGAND;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CHO CELL; CONTROLLED STUDY; DRUG RECEPTOR BINDING; FLUORESCENCE; GENE TRANSFER; IN VITRO STUDY; INTERMETHOD COMPARISON; LASER MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RECEPTOR DENSITY; TECHNIQUE;

ANIMALS; ARTIFACTS; BINDING, COMPETITIVE; CHO CELLS; CLONING, MOLECULAR; CONTRAST MEDIA; CRICETINAE; FLUORESCEIN; KINETICS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NALOXONE; NARCOTIC ANTAGONISTS; RADIOLIGAND ASSAY; RECEPTORS, OPIOID, MU; SENSITIVITY AND SPECIFICITY; TRANSFECTION; TRITIUM;

EID: 0034655706     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(00)00175-8     Document Type: Article

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