Antimicrobials from herbs, spices, and plants

Encapsulations: Nanotechnology in the Agri-Food Industry: Volume 2

Volumn 2, Issue , 2016, Pages 269-293

  Bor, Tarik ^a   Aljaloud, Sulaiman O ^a,b   Gyawali, Rabin ^a   Ibrahim, Salam A ^a  

^a NORTH CAROLINA A AND T STATE UNIVERSITY   (United States)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

encapsulation; essential oil; nanodispersion; nanoencapsulation; natural antimicrobials

Indexed keywords

EID: 85097671105     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-0-12-804307-3.00007-7     Document Type: Chapter

References (104)

1. M. Akhtar and E. Dickinson (2003) Emulsifying properties of whey protein–dextran conjugates at low pH and different salt concentrations. Colloid. Surf. B 31 125-132.
2. M. Akhtar and E. Dickinson (2007) Whey protein–maltodextrin conjugates as emulsifying agents: an alternative to gum arabic. Food Hydrocoll. 21 607-616.
3. K. Anjani, K. Kailasapathy and M. Phillips (2007) Microencapsulation of enzymes for potential application in acceleration of cheese ripening. Int. Dairy J. 17 79-86.
4. M.A. Augustin and Y. Hemar (2009) Nano- and micro-structured assemblies for encapsulation of food ingredients. Chem. Soc. Rev. 38 902-912.
5. E.K. Bae and S.J. Lee (2008) Microencapsulation of avocado oil by spray drying using whey protein and maltodextrin. J. Microencapsul. 25 549-560.
6. R. Baranauskiene, P.R. Venskutonis, K. Dewettinck and R. Verhe (2006) Properties of oregano (Origanum vulgare L.), citronella (Cymbopogon nardus G.) and marjoram (Majorana hortensis L.) flavors encapsulated into milk protein-based matrices. Food Res. Int. 39 413-425.
7. M.I.M.J. Barbosa, C.D. Borsarelli and A.Z. Mercadante (2005) Light stability of spray-dried bixin encapsulated with different edible polysaccharide preparations. Food Res. Int. 38 989-994.
8. S. Burt (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int. J. Food Microbiol. 94 223-253.
9. C. Butstraen and F. Salaun (2014) Preparation of microcapsules by complex coacervation of gum arabic and chitosan. Carbohydr. Polym. 99 608-616.
10. P. Calvo, T. Hernandez, M. Lozano and D. Gonzalez-Gomez (2010) Microencapsulation of extra-virgin olive oil by spray-drying: influence of wall material and olive quality. Eur. J. Lipid Sci. Technol. 112 852-858.
11. H.C.F. Carneiro, R.V. Tonon, C.R.F. Grosso and M.D. Hubinger (2013) Encapsulation efficiency and oxidative stability of flaxseed oil microencapsulated by spray drying using different combinations of wall materials. J. Food Eng. 115 443-451.
12. P.P. Cevallos P.a.P., M.P. Buera and B.E. Elizalde (2010) Encapsulation of cinnamon and thyme essential oils components (cinnamaldehyde and thymol) in β-cyclodextrin—effect of interactions with water on complex stability. J. Food Eng. 99 70-75.
13. M. Chavarri, I. Maranon, R. Ares, F.C. Ibanez, F. Marzo and M.D.C. Villaran (2010) Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. Int. J. Food Microbiol. 142 185-189.
14. W.S. Cheow and K. Hadinoto (2013) Biofilm-like Lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance. Biomacromolecules 14 3214-3222.
15. Y.T. Chiu, C.P. Chiu, J.T. Chien, G.H. Ho, J. Yang and B.H. Chen (2007) Encapsulation of lycopene extract from tomato pulp waste with gelatin and poly(γ-glutamic acid) as carrier. J. Agric. Food Chem. 55 5123-5130.
16. M.-J. Choi, U. Ruktanonchai, S.-G. Min, J.-Y. Chun and A. Soottitantawat (2010) Physical characteristics of fish oil encapsulated by β-cyclodextrin using an aggregation method or polycaprolactone using an emulsion–diffusion method. Food Chem. 119 1694-1703.
17. K.-O. Choi, J. Ryu, H.-S. Kwak and S. Ko (2010) Spray-dried conjugated linoleic acid encapsulated with Maillard reaction products of whey proteins and maltodextrin. Food Sci. Biotechnol. 19 957-965.
18. C. Chranioti and C. Tzia (2013) Arabic gum mixtures as encapsulating agents of freeze-dried fennel oleoresin products. Food Bioprocess Technol. 7 1057-1065.
19. T.A. Costa-Silva, S. Said, C.R.F. Souza and W.P. Oliveira (2010) Stabilization of endophytic fungus Cercospora kikuchii lipase by spray drying in the presence of maltodextrin and β-cyclodextrin. Dry. Technol. 28 1245-1254.
20. F.P. De Castro-Cislaghi, C.D.R.E. Silva, C.B. Fritzen-Freire, J.G. Lorenz and E.S. Sant’anna (2012) Bifidobacterium bb-12 microencapsulated by spray drying with whey: survival under simulated gastrointestinal conditions, tolerance to nacl, and viability during storage. J. Food Eng. 113 186-193.
21. K. Dewettinck and A. Huyghebaert (1999) Fluidized bed coating in food technology. Trends Food Sci. Technol. 10 163-168.
22. E. Dickinson (2003) Hydrocolloids at interfaces and the influence on the properties of dispersed systems. Food Hydrocoll. 17 25-39.
23. Z. Dong, Y. Ma, K. Hayat, C. Jia, S. Xia and X. Zhang (2011) Morphology and release profile of microcapsules encapsulating peppermint oil by complex coacervation. J. Food Eng. 104 455-460.
24. F. Donsi, M. Annunziata, M. Sessa and G. Ferrari (2011) Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT Food Sci. Technol. 44 1908-1914.
25. F. Donsì, M. Annunziata, M. Vincensi and G. Ferrari (2012) Design of nanoemulsion-based delivery systems of natural antimicrobials: effect of the emulsifier. J. Biotechnol. 159 342-350.
26. A.R. Donthidi, R.F. Tester and K.E. Aidoo (2010) Effect of lecithin and starch on alginate-encapsulated probiotic bacteria. J. Microencapsul. 27 67-77.
27. S. Drusch, S. Berg, M. Scampicchio, Y. Serfert, V. Somoza, S. Mannino and K. Schwarz (2009) Role of glycated caseinate in stabilisation of microencapsulated lipophilic functional ingredients. Food Hydrocoll. 23 942-948.
28. G. Elshafei, M.M. El-Said, H.A.E. Attia and T.G.M. Mohammed (2010) Environmentally friendly pesticides: essential oil-based w/o/w multiple emulsions for anti-fungal formulations. Ind. Crop. Prod. 31 99-106.
29. P.N. Ezhilarasi, D. Indrani, B.S. Jena and C. Anandharamakrishnan (2013) Freeze-drying technique for microencapsulation of Garcinia fruit extract and its effect on bread quality. J. Food Eng. 117 513-520.
30. B. Farhang (2009) Nanotechnology and applications in food safety. B.-C. Gustavo, M. Alan, L. David, S. Walter, B. Ken, C. Paul (Eds) Global Issues in Food Science and Technology San Diego, CA: Academic Press
31. A.L. Gabas, V.R.N. Telis, P.J.A. Sobral and J. Telis-Romero (2007) Effect of maltodextrin and arabic gum in water vapor sorption thermodynamic properties of vacuum dried pineapple pulp powder. J. Food Eng. 82 246-252.
32. E. Gavini, V. Sanna, R. Sharma, C. Juliano, M. Usai, M. Marchetti, J. Karlsen and P. Giunchedi (2005) Solid lipid microparticles (SLM) containing juniper oil as anti-acne topical carriers: preliminary studies. Pharm. Dev. Technol. 10 479-487.
33. G.K. Gbassi, T. Vandamme, S. Ennahar and E. Marchioni (2009) Microencapsulation of Lactobacillus plantarum spp. in an alginate matrix coated with whey proteins. Int. J. Food Microb. 129 103-105.
34. A. Gharsallaoui, G. Roudaut, O. Chambin, A. Voilley and R. Saurel (2007) Applications of spray-drying in microencapsulation of food ingredients: an overview. Food Res. Int. 40 1107-1121.
35. G.M. Glenn, A.P. Klamczynski, D.F. Woods, B. Chiou, W.J. Orts and S.H. Imam (2010) Encapsulation of plant oils in porous starch microspheres. J. Agric. Food Chem. 58 4180-4184.
36. O. Gortzi, S. Lala, I. Chinou and J. Tsaknis (2007) Evaluation of the antimicrobial and antioxidant activities of Origanum dictamnus extracts before and after encapsulation in liposomes. Molecules 12 932-945.
37. B. Guignon, A. Duquenoy and E.D. Dumoulin (2002) Fluid bed encapsulation of particles: principles and practice. Dry. Technol. 20 419-447.
38. J. Gutierrez, C. Barry-Ryan and P. Bourke (2008) The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int. J. Food Microb. 124 91-97.
39. R. Gyawali, S.A. Hayek and S.A. Ibrahim (2014) Plant extracts as antimicrobials in food products: types. Cambridge, UK: Woodhead Publishing p. 31
40. R. Gyawali, S.A. Hayek and S.A. Ibrahim (2014) Plant extracts as antimicrobials in food products: mechanisms of action, extraction methods, and applications. Cambridge, UK: Woodhead Publishing p. 49
41. R. Gyawali and S.A. Ibrahim (2012) Impact of plant derivatives on the growth of foodborne pathogens and the functionality of probiotics. Appl. Microbiol. Biotechnol. 95(1), 29-45.
42. R. Gyawali and S.A. Ibrahim (2014) Natural products as antimicrobial agents. Food Control 46 412-429.
43. R. Gyawali, S.A. Ibrahim, S.H.A. Hasfa, S.Q. Smqadri and Y. Haik (2011) Antimicrobial activity of copper alone and in combination with lactic acid against Escherichia coli O157:H7 in laboratory medium and on the surface of lettuce and tomatoes. J. Pathog. 2011 650968.
44. S.M. Jafari, E. Assadpoor, B. Bhandari and Y. He (2008) Encapsulation efficiency of food flavours and oils during spray drying. Dry. Technol. 26 816-835.
45. A. Jagannath, P.S. Raju and A.S. Bawa (2010) Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria. LWT Food Sci. Technol. 43 1197-1203.
46. X. Jun-Xia, Y. Hai-Yan and Y. Jian (2011) Microencapsulation of sweet orange oil by complex coacervation with soybean protein isolate/gum Arabic. Food Chem. 125 1267-1272.
47. N. Kalogeropoulos, K. Yannakopoulou, A. Gioxari, A. Chiou and D.P. Makris (2010) Polyphenol characterization and encapsulation in β-cyclodextrin of a flavonoid-rich Hypericum perforatum (St. John’s wort) extract. LWT Food Sci. Technol. 43 882-889.
48. D. Kanakdande, R. Bhosale and R.S. Singhal (2007) Stability of cumin oleoresin microencapsulated in different combination of gum arabic, maltodextrin and modified starch. Carbohydr. Polym. 67 536-541.
49. A. Kato (2002) Industrial applications of Maillard-type protein-polysaccharide conjugates. Food Sci. Technol. Res. 8 193-199.
50. V. Kaushik and Y.H. Roos (2007) Limonene encapsulation in freeze-drying of gum Arabic–sucrose–gelatin systems. LWT Food Sci. Technol. 40 1381-1391.
51. S. Krishnan, R. Bhosale and R.S. Singhal (2005) Microencapsulation of cardamom oleoresin: evaluation of blends of gum arabic, maltodextrin and a modified starch as wall materials. Carbohydr. Polym. 61 95-102.
52. F.V. Leimann, O.H. Gonçalves, R.A.F. Machado and A. Bolzan (2009) Antimicrobial activity of microencapsulated lemongrass essential oil and the effect of experimental parameters on microcapsules size and morphology. Mater. Sci. Eng. C 29 430-436.
53. X.-Y. Li, L.-J. Jin, T.A. Mcallister, K. Stanford, J.-Y. Xu, Y.-N. Lu, Y.-H. Zhen, Y.-X. Sun and Y.-P. Xu (2007) Chitosan–alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY). J. Agric. Food Chem. 55 2911-2917.
54. C.C. Liolios, O. Gortzi, S. Lalas, J. Tsaknis and I. Chinou (2009) Liposomal incorporation of carvacrol and thymol isolated from the essential oil of Origanum dictamnus L. and in vitro antimicrobial activity. Food Chem. 112 77-83.
55. Y. Lv, F. Yang, X. Li, X. Zhang and S. Abbas (2014) Formation of heat-resistant nanocapsules of jasmine essential oil via gelatin/gum arabic based complex coacervation. Food Hydrocoll. 35 305-314.
56. C.R. Malacrida, S. Ferreira, N. Telis and R. Vania (2013) Stability at different temperatures of turmeric oleoresin encapsulated in maltodextrin/gelatin matrices by freeze-drying. J. Colloid Sci. Biotechnol. 2 100-105.
57. I.M. Martins, S.N. Rodrigues, F. Barreiro and A.E. Rodrigues (2009) Microencapsulation of thyme oil by coacervation. J. Microencapsul. 26 667-675.
58. D.J. McClements, E.A. Decker and J. Weiss (2007) Emulsion-based delivery systems for lipophilic bioactive components. J. Food Sci. 72 R109-R124.
59. I. Mourtzinos, N. Kalogeropoulos, S.E. Papadakis, K. Konstantinou and V.T. Karathanos (2008) Encapsulation of nutraceutical monoterpenes in β-cyclodextrin and modified starch. J. Food Sci. 73 S89-S94.
60. I. Mourtzinos, F. Salta, K. Yannakopoulou, A. Chiou and V.T. Karathanos (2007) Encapsulation of olive leaf extract in β-cyclodextrin. J. Agric. Food Chem. 55 8088-8094.
61. R. Murugesan and V. Orsat (2011) Spray drying of elderberry (Sambucus nigra L.) juice to maintain its phenolic content. Dry. Technol. 29 1729-1740.
62. K. Nakagawa and H. Nagao (2012) Microencapsulation of oil droplets using freezing-induced gelatin–acacia complex coacervation. Colloids Surf. A 411 129-139.
63. M.P. Nori, C.S. Favaro-Trindade, S.M. De Alencar, M. Thomazini, J.C. De Camargo Balieiro and C.J.C. Castillo (2011) Microencapsulation of propolis extract by complex coacervation. LWT Food Sci. Technol. 44 429-435.
64. I.L. Nunes and A.Z. Mercadante (2007) Encapsulation of lycopene using spray-drying and molecular inclusion processes. Braz. Arch. Biol. Technol. 50 893-900.
65. F. Ozyildiz, S. Karagonlu, G. Basal, A. Uzel and O. Bayraktar (2013) Micro-encapsulation of ozonated red pepper seed oil with antimicrobial activity and application to nonwoven fabric. Lett. Appl. Microbiol. 56 168-179.
66. E.I. Paramera, S.J. Konteles and V.T. Karathanos (2011) Stability and release properties of curcumin encapsulated in Saccharomyces cerevisiae β-cyclodextrin and modified starch. Food Chem. 125 913-922.
67. A.A. Perez, C.R. Carrara, C.C. Sanchez, L.G. Santiago and J.M.R. Patino (2009) Interfacial dynamic properties of whey protein concentrate/polysaccharide mixtures at neutral pH. Food Hydrocoll. 23 1253-1262.
68. A. Poshadri and A. Kuna (2010) Microencapsulation technology: a review. J. Res. Angrau 38 86-102.
69. R. Rajam, P. Karthik, S. Parthasarathi, G.S. Joseph and C. Anandharamakrishnan (2012) Effect of whey protein—alginate wall systems on survival of microencapsulated Lactobacillus plantarum in simulated gastrointestinal conditions. J. Funct. Foods 4 891-898.
70. M.P. Rascon, C.I. Beristain, H.S. García and M.A. Salgado (2011) Carotenoid retention and storage stability of spray-dried encapsulated paprika oleoresin using gum arabic and soy protein isolate as wall materials. LWT Food Sci. Technol. 44 549-557.
71. P. Robert, T. Gorena, N. Romero, E. Sepulveda, J. Chavez and C. Saenz (2010) Encapsulation of polyphenols and anthocyanins from pomegranate (Punica granatum) by spray drying. Int. J. Food Sci. Technol. 45 1386-1394.
72. D.A. Rodea-Gonzalez, J. Cruz-Olivares, A. Roman-Guerrero, M.E. Rodriguez-Huezo, E.J. Vernon-Carter and C. Perez-Alonso (2012) Spray-dried encapsulation of chia essential oil (Salvia hispanica L.) in whey protein concentrate-polysaccharide matrices. J. Food Eng. 111 102-109.
73. A. Romo-Hualde, A.I. Yetano-Cunchillos, C. González-Ferrero, M.J. Saiz-Abajo and C.J. Gonzalez-Navarro (2012) Supercritical fluid extraction and microencapsulation of bioactive compounds from red pepper (Capsicum annum L.) by-products. Food Chem. 133 1045-1049.
74. A. Sahu, N. Kasoju, P. Goswami and U. Bora (2011) Encapsulation of curcumin in pluronic block copolymer micelles for drug delivery applications. J. Biomater. Appl. 25 619-639.
75. V. Sanchez, R. Baeza, M.V. Galmarini, M.C. Zamora and J. Chirife (2011) Freeze-drying encapsulation of red wine polyphenols in an amorphous matrix of maltodextrin. Food Bioprocess Technol. 6 1350-1354.
76. E.-J. Seo, S.-G. Min and M.-J. Choi (2010) Release characteristics of freeze-dried eugenol encapsulated with β-cyclodextrin by molecular inclusion method. J. Microencapsul. 27 496-505.
77. Shah, B.D., 2011. Nanodispersing lipophilic antimicrobials for improved food safety. PhD Dissertation. The University of Tennessee, Knoxville, TN.
78. B. Shah, P.M. Davidson and Q. Zhong (2012) Nanocapsular dispersion of thymol for enhanced dispersibility and increased antimicrobial effectiveness against Escherichia coli O157:H7 and Listeria monocytogenes in model food systems. Appl. Environ. Microbiol. 78 8448-8453.
79. B.D. Shah, P.M. Davidson and Q. Zhong (2012) Encapsulation of eugenol using Maillard-type conjugates to form transparent and heat stable nanoscale dispersions. LWT Food Sci. Technol. 49 139-148.
80. B.D. Shah, P.M. Davidson and Q. Zhong (2013) Nanodispersed eugenol has improved antimicrobial activity against Escherichia coli O157:H7 and Listeria monocytogenes in bovine milk. Int. J. Food Microb. 161 53-59.
81. B. Shah, S. Ikeda, P.M. Davidson and Q. Zhong (2012) Nanodispersing thymol in whey protein isolate-maltodextrin conjugate capsules produced using the emulsion–evaporation technique. J. Food Eng. 113 79-86.
82. J. Shaikh, R. Bhosale and R.S. Singhal (2006) Microencapsulation of black pepper oleoresin. Food Chem. 94 105-110.
83. U. Shinde and M. Nagarsenker (2011) Microencapsulation of eugenol by gelatin-sodium alginate complex coacervation. Indian J. Pharm. Sci. 73 311-315.
84. D.F. Silva, C.S. Favaro-Trindade, G.A. Rocha and M. Thomazini (2012) Microencapsulation of lycopene by gelatin–pectin complex coacervation. J. Food Process. Preserv. 36 185-190.
85. A. Soottitantawat, F. Bigeard, H. Yoshii, T. Furuta, M. Ohkawara and P. Linko (2005) Influence of emulsion and powder size on the stability of encapsulated d-limonene by spray drying. Innov. Food Sci. Emerg. Technol. 6 107-114.
86. M. Sousdaleff, M.L. Baesso, A.M. Neto, A.C. Nogueira, V.A. Marcolino and G. Matioli (2012) Microencapsulation by freeze-drying of potassium norbixinate and curcumin with maltodextrin: stability, solubility, and food application. J. Agric. Food Chem. 61 955-965.
87. J.C. Spada, L.D.F. Marczak, I.C. Tessaro and C.P.Z. Noreña (2012) Microencapsulation of β-carotene using native pinhão starch, modified pinhão starch and gelatin by freeze-drying. Int. J. Food Sci. Technol. 47 186-194.
88. S.C. Sutter, M.P. Buera and B.E. Elizalde (2007) β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion. Int. J. Pharm. 332 45-54.
89. C.C. Tassou, E.H. Drosinos and G.J.E. Nychas (1995) Effects of essential oil from mint (Mentha piperita) on Salmonella enteritidis and Listeria monocytogenes in model food systems at 4° and 10°C. J. Appl. Microbiol. 78 593-600.
90. M.I. Teixeira, L.R. Andrade, M. Farina and M.H.M. Rocha-Leao (2004) Characterization of short chain fatty acid microcapsules produced by spray drying. Mater. Sci. Eng. C 24 653-658.
91. R.V. Tonon, C.R.F. Grosso and M.D. Hubinger (2011) Influence of emulsion composition and inlet air temperature on the microencapsulation of flaxseed oil by spray drying. Food Res. Int. 44 282-289.
92. A. Toure, H.B. Lu, X. Zhang and X. Xueming (2011) Microencapsulation of ginger oil in 18DE maltodextrin/whey protein isolate. J. Herbs Spices Med. Plants 17 183-195.
93. S. Vaidya, R. Bhosale and R.S. Singhal (2006) Microencapsulation of cinnamon oleoresin by spray drying using different wall materials. Dry. Technol. 24 983-992.
94. S. Varona, A. Martin and M.J. Cocero (2011) Liposomal incorporation of lavandin essential oil by a thin-film hydration method and by particles from gas-saturated solutions. Ind. Eng. Chem. Res. 50 2088-2097.
95. T. Wang and J.A. Lucey (2003) Use of multi-angle laser light scattering and size-exclusion chromatography to characterize the molecular weight and types of aggregates present in commercial whey protein products. J. Dairy Sci. 86 3090-3101.
96. R. Wang, Z. Tian and L. Chen (2011) A novel process for microencapsulation of fish oil with barley protein. Food Res. Int. 44 2735-2741.
97. W.A. Wannes, B. Mhamdi, J. Sriti, M. Ben Jemia, O. Ouchikh, G. Hamdaoui, M.E. Kchouk and B. Marzouk (2010) Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food Chem. Toxicol. 48 1362-1370.
98. J. Weiss, S. Gaysinsky, M. Davidson and J. McClements (2009) Nanostructured encapsulation systems: food antimicrobials. B.-C. Gustavo, M. Alan, L. David, S. Walter, B. Ken, C. Paul (Eds) Global Issues in Food Science and Technology San Diego, CA: Academic Press (Chapter 24)
99. J. Weiss, P. Takhistov and D.J. McClements (2006) Functional materials in food nanotechnology. J. Food Sci. 71 R107-R116.
100. D.Y. Ying, J. Sun, L. Sanguansri, R. Weerakkody and M.A. Augustin (2012) Enhanced survival of spray-dried microencapsulated Lactobacillus rhamnosus GG in the presence of glucose. J. Food Eng. 109 597-602.
101. P.A. Yoshida, D. Yokota, M.A. Foglio, R.A.F. Rodrigues and S.C. Pinho (2010) Liposomes incorporating essential oil of Brazilian cherry (Eugenia uniflora L.): characterization of aqueous dispersions and lyophilized formulations. J. Microencapsul. 27 416-425.
102. H. Yu and Q. Huang (2010) Enhanced in vitro anticancer activity of curcumin encapsulated in hydrophobically modified starch. Food Chem. 119 669-674.
103. C. Yu, W. Wang, H. Yao and H. Liu (2007) Preparation of phospholipid microcapsule by spray drying. Dry. Technol. 25 695-702.
104. W. Zhang, J. Rong, Q.I.N. Wang and X. He (2009) The encapsulation and intracellular delivery of trehalose using a thermally responsive nanocapsule. Nanotechnology 20 275101.