How to design a single-cell RNA-sequencing experiment: Pitfalls, challenges and perspectives

Briefings in Bioinformatics

Volumn 20, Issue 4, 2018, Pages 1384-1394

  Dal Molin, Alessandra ^a   Di Camillo, Barbara ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

experimental design; experimental protocols; normalization; single cell RNA seq; spike ins; Unique Molecular Identifiers

Indexed keywords

MESSENGER RNA; TRANSCRIPTOME;

ANIMAL; BIOLOGY; CELL SEPARATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; NUCLEIC ACID AMPLIFICATION; PROCEDURES; QUALITY CONTROL; REVERSE TRANSCRIPTION; SINGLE CELL ANALYSIS;

ANIMALS; CELL SEPARATION; COMPUTATIONAL BIOLOGY; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; QUALITY CONTROL; REVERSE TRANSCRIPTION; RNA, MESSENGER; RNA-SEQ; SINGLE-CELL ANALYSIS; TRANSCRIPTOME;

EID: 85072991191     PISSN: 14675463     EISSN: 14774054     Source Type: Journal    
DOI: 10.1093/bib/bby007     Document Type: Article

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