Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

Nature Methods

Volumn 11, Issue 4, 2014, Pages 429-435

  Guilinger, John P ^a,b   Pattanayak, Vikram ^a,b   Reyon, Deepak ^c,d   Tsai, Shengdar Q ^c,d   Sander, Jeffry D ^c,d   Joung, J Keith ^c,d   Liu, David R ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ATM PROTEIN; CHEMOKINE RECEPTOR CCR5; NUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; COMPUTER ANALYSIS; CONTROLLED STUDY; DNA BINDING; DNA CLEAVAGE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME SPECIFICITY; ENZYME STRUCTURE; GENE TARGETING; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; HUMAN GENOME; IN VITRO SELECTION; PRIORITY JOURNAL; PROTEIN DOMAIN;

BASE SEQUENCE; BINDING SITES; CELL LINE; DEOXYRIBONUCLEASES; DNA; GENE TARGETING; HUMANS; PROTEIN BINDING; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 84897954502     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.2845     Document Type: Article

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