-
1
-
-
0020480472
-
Chemical synthesis and cloning of a gene for human β-urogastrone
-
Smith, J. et al. Chemical synthesis and cloning of a gene for human β-urogastrone. Nucleic Acids Res. 10, 4467-4482 (1982).
-
(1982)
Nucleic Acids Res
, vol.10
, pp. 4467-4482
-
-
Smith, J.1
-
2
-
-
17944400201
-
Chemical synthesis of a human interferon-α2 gene and its expression in Escherichia coli
-
Edge, M.D. et al. Chemical synthesis of a human interferon-α2 gene and its expression in Escherichia coli. Nucleic Acids Res. 11, 6419-6435 (1983).
-
(1983)
Nucleic Acids Res
, vol.11
, pp. 6419-6435
-
-
Edge, M.D.1
-
3
-
-
0021220804
-
Chemical synthesis of a biologically active gene for human immune interferon-γ. Prospect for site-specific mutagenesis and structure-function studies
-
Jay, E. et al. Chemical synthesis of a biologically active gene for human immune interferon-γ. Prospect for site-specific mutagenesis and structure-function studies. J. Biol. Chem. 259, 6311-6317 (1984).
-
(1984)
J. Biol. Chem
, vol.259
, pp. 6311-6317
-
-
Jay, E.1
-
4
-
-
0022432043
-
Chemical synthesis of a gene for somatomedin C
-
Sproat, B.S. & Gait M.J. Chemical synthesis of a gene for somatomedin C. Nucleic Acids Res. 13, 2959-2977 (1985).
-
(1985)
Nucleic Acids Res
, vol.13
, pp. 2959-2977
-
-
Sproat, B.S.1
Gait, M.J.2
-
5
-
-
0023737075
-
FokI method of gene synthesis
-
Mandecki, W. & Boiling, T.J. FokI method of gene synthesis. Gene 68, 101-107 (1988).
-
(1988)
Gene
, vol.68
, pp. 101-107
-
-
Mandecki, W.1
Boiling, T.J.2
-
6
-
-
0024995923
-
A rapid method for the construction of synthetic genes using the polymerase chain reaction
-
Dillon, P.J. & Rosen, C.A. A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques 9, 298-300 (1990).
-
(1990)
Biotechniques
, vol.9
, pp. 298-300
-
-
Dillon, P.J.1
Rosen, C.A.2
-
7
-
-
0027050148
-
Recursive PCR: A novel technique for total gene synthesis
-
Prodromou, C. & Pearl, L.H. Recursive PCR: A novel technique for total gene synthesis. Protein Eng. 5, 827-829 (1992).
-
(1992)
Protein Eng
, vol.5
, pp. 827-829
-
-
Prodromou, C.1
Pearl, L.H.2
-
8
-
-
0026006502
-
Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands
-
Ciccarelli, R.B., Gunyuzlu, P., Huang, J., Scott, C. & Oakes, F.T. Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands. Nucleic Adds Res. 19 6007-6013 (1991).
-
(1991)
Nucleic Adds Res
, vol.19
, pp. 6007-6013
-
-
Ciccarelli, R.B.1
Gunyuzlu, P.2
Huang, J.3
Scott, C.4
Oakes, F.T.5
-
9
-
-
0028784138
-
Single-step assembly of a gene and entire plasmid from large numbers of oligonucleotides
-
Stemmer, W.P., Crameri, A., Ha, K.D., Brennan, T.M. & Heyneker, H.L. Single-step assembly of a gene and entire plasmid from large numbers of oligonucleotides. Gene 164, 49-53 (1995).
-
(1995)
Gene
, vol.164
, pp. 49-53
-
-
Stemmer, W.P.1
Crameri, A.2
Ha, K.D.3
Brennan, T.M.4
Heyneker, H.L.5
-
10
-
-
12644281616
-
A synthetic cryIC gene, encoding a Bacillus thuringiensis δ-endotoxin, confers Spodoptera resistance in alfalfa and tobacco
-
Strizhov, N. et al. A synthetic cryIC gene, encoding a Bacillus thuringiensis δ-endotoxin, confers Spodoptera resistance in alfalfa and tobacco. Proc. Natl. Acad. Sci. USA 93, 15012-15017 (1996).
-
(1996)
Proc. Natl. Acad. Sci. USA
, vol.93
, pp. 15012-15017
-
-
Strizhov, N.1
-
11
-
-
2142799564
-
Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: A novel method of primer design for high-fidelity assembly of longer gene sequences
-
Gao, X., Yo, P., Keith, A., Ragan, T.J. & Harris, T.K. Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: A novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Res. 31, e143 (2003).
-
(2003)
Nucleic Acids Res
, vol.31
-
-
Gao, X.1
Yo, P.2
Keith, A.3
Ragan, T.J.4
Harris, T.K.5
-
12
-
-
0026502381
-
Dual asymmetric PCR: One-step construction of synthetic genes
-
Sandhu, G.S., Aleff, R.A. & Kline, B.C. Dual asymmetric PCR: One-step construction of synthetic genes. Biotechniques 12, 14-16 (1992).
-
(1992)
Biotechniques
, vol.12
, pp. 14-16
-
-
Sandhu, G.S.1
Aleff, R.A.2
Kline, B.C.3
-
13
-
-
17044455338
-
Two-step total gene synthesis method
-
Young, L. & Dong, Q. Two-step total gene synthesis method. Nucleic Acids Res. 32, e59 (2004).
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Young, L.1
Dong, Q.2
-
14
-
-
0013024192
-
Synthesis and sequence determination of ACC deaminase gene using successive expressive extension PCR method
-
Yao, Q.H., Huang, X.M., Peng, R.H. & Fan, H.Q. Synthesis and sequence determination of ACC deaminase gene using successive expressive extension PCR method. Acta Agricultural Shanghai. 15, 11-16 (1999).
-
(1999)
Acta Agricultural Shanghai
, vol.15
, pp. 11-16
-
-
Yao, Q.H.1
Huang, X.M.2
Peng, R.H.3
Fan, H.Q.4
-
15
-
-
3042820410
-
A simple, rapid, high fidelity and cost-effective PCR-based two-step DNA synthesis (PTDS) method for long gene sequences
-
Xiong, A.S. et al. A simple, rapid, high fidelity and cost-effective PCR-based two-step DNA synthesis (PTDS) method for long gene sequences. Nucleic Acids Res. 32, e98 (2004).
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Xiong, A.S.1
-
16
-
-
13244268400
-
High level expression of a recombinant acid phytase gene in Pichia pastoris. 3
-
Xiong, A.S. et al. High level expression of a recombinant acid phytase gene in Pichia pastoris. 3. Appl. Microbiol. 98, 418-428 (2005).
-
(2005)
Appl. Microbiol
, vol.98
, pp. 418-428
-
-
Xiong, A.S.1
-
17
-
-
32944470591
-
Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola
-
Peng, R.H., Yao, Q.H., Xiang, A.S., Cheng, Z.M. & Li Y. Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola. Plant Cell Rep. 25, 124-132 (2006).
-
(2006)
Plant Cell Rep
, vol.25
, pp. 124-132
-
-
Peng, R.H.1
Yao, Q.H.2
Xiang, A.S.3
Cheng, Z.M.4
Li, Y.5
-
18
-
-
3142666033
-
Isolation, characterization, molecular cloning of the cDNA encoding a novel phytase from Aspegillus niger 113 and high expression in Pichia pastoris
-
Xiong, A.S. et al. Isolation, characterization, molecular cloning of the cDNA encoding a novel phytase from Aspegillus niger 113 and high expression in Pichia pastoris. J. Biochem. Mol. Biol. 37 282-291 (2004).
-
(2004)
J. Biochem. Mol. Biol
, vol.37
, pp. 282-291
-
-
Xiong, A.S.1
-
19
-
-
0347364647
-
Generating a synthetic genome by whole genome assembly: PhiX174 bacteriophage from synthetic otigonucteotides
-
Smith, H.O., Hutchison, C.A., Pfannkoch, C. & Venter, J.C. Generating a synthetic genome by whole genome assembly: PhiX174 bacteriophage from synthetic otigonucteotides. Proc. Natl. Acad. Sci. USA 100, 15440-15445 (2003).
-
(2003)
Proc. Natl. Acad. Sci. USA
, vol.100
, pp. 15440-15445
-
-
Smith, H.O.1
Hutchison, C.A.2
Pfannkoch, C.3
Venter, J.C.4
-
20
-
-
16544393254
-
Protein-mediated error correction for de novo DNA synthesis
-
Carr, P.A. et al. Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 32, e162 (2004).
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Carr, P.A.1
-
21
-
-
33749163829
-
-
Xiong, A.S. et al. High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. published online 7 April 2006 (doi:110.1007/s00253-006-0384-8).
-
Xiong, A.S. et al. High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. published online 7 April 2006 (doi:110.1007/s00253-006-0384-8).
-
-
-
-
22
-
-
0037095730
-
DNAWorks: An automated method-for designing oligonucleotides for PCR-based gene synthesis
-
Hoover, D.M. & Lubkowski, J. DNAWorks: An automated method-for designing oligonucleotides for PCR-based gene synthesis. Nucleic Acids Res. 30, e43 (2002).
-
(2002)
Nucleic Acids Res
, vol.30
-
-
Hoover, D.M.1
Lubkowski, J.2
-
23
-
-
28644442082
-
Correcting errors in synthetic DNA through consensus shuffling
-
Binkowski, B.F., Richmond, K.E., Kaysen, J., Sussman, M.R. & Belshaw, P.J. Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 33, e55 (2005).
-
(2005)
Nucleic Acids Res
, vol.33
-
-
Binkowski, B.F.1
Richmond, K.E.2
Kaysen, J.3
Sussman, M.R.4
Belshaw, P.J.5
-
24
-
-
0242442569
-
DNA mismatch repair molecular mechanisms and biological function
-
Schofield, M.J. & Hsieh, P. DNA mismatch repair molecular mechanisms and biological function. Annu. Rev. Microbiol. 57, 579-608 (2003).
-
(2003)
Annu. Rev. Microbiol
, vol.57
, pp. 579-608
-
-
Schofield, M.J.1
Hsieh, P.2
-
25
-
-
19644370646
-
An advanced software package for designing synthetic genes
-
Jayaraj, S., Reid, R. & Santi, D.V. GeMS: An advanced software package for designing synthetic genes. Nucleic Acids Res. 33, 3011-3016 (2005).
-
(2005)
Nucleic Acids Res
, vol.33
, pp. 3011-3016
-
-
Jayaraj, S.1
Reid, R.2
Santi, D.V.G.3
-
26
-
-
0004136246
-
-
Sambrook, J. & Russell, D.W, eds, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
-
Sambrook, J. & Russell, D.W. (eds) Molecular Cloning; A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001).
-
(2001)
Molecular Cloning; A Laboratory Manual
-
-
-
28
-
-
0030786334
-
A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR
-
Urban, A., Neukirchen, S. & Jaeger K.E. A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR. Nucleic Acids Res. 25, 2227-228 (1997).
-
(1997)
Nucleic Acids Res
, vol.25
, pp. 2227-2228
-
-
Urban, A.1
Neukirchen, S.2
Jaeger, K.E.3
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