Selection and characterization of DARPins specific for the neurotensin receptor 1

Protein Engineering, Design and Selection

Volumn 22, Issue 6, 2009, Pages 357-366

  Milovnik, Peter ^a   Ferrari, Davide ^a,b   Sarkar, Casim A ^a,c   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF PARMA   (Italy)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

DARPins; GPCR; Neurotensin; NTR1; Ribosome display

Indexed keywords

DARPINS; GPCR; NEUROTENSIN; NTR1; RIBOSOME DISPLAY;

AMINES; AMINO ACIDS; BINDING ENERGY; CHROMATOGRAPHIC ANALYSIS; CLONING; DETERGENTS; FLUORESCENCE MICROSCOPY; MAMMALS; ORGANIC ACIDS; SIGNAL TRANSDUCTION; SIZE EXCLUSION CHROMATOGRAPHY;

BIOLOGICAL MEMBRANES;

ANKYRIN; DESIGNED ANKYRIN REPEAT PROTEIN; G PROTEIN COUPLED RECEPTOR; NEUROTENSIN RECEPTOR; NEUROTENSIN RECEPTOR 1; UNCLASSIFIED DRUG; NEUROTENSIN TYPE 1 RECEPTOR; PEPTIDE LIBRARY; RECOMBINANT PROTEIN;

ARTICLE; BINDING AFFINITY; CELL MEMBRANE; CLONING; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; FLUORESCENCE MICROSCOPY; GEL PERMEATION CHROMATOGRAPHY; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; RADIOIMMUNOASSAY; RIBOSOME; WESTERN BLOTTING; ANIMAL; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; RADIOASSAY; RAT;

MAMMALIA; RATTUS;

ANIMALS; ANKYRIN REPEAT; CERCOPITHECUS AETHIOPS; CHROMATOGRAPHY, GEL; COS CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; ESCHERICHIA COLI; MICROSCOPY, FLUORESCENCE; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN ENGINEERING; RADIOLIGAND ASSAY; RATS; RECEPTORS, NEUROTENSIN; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 67651094146     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp011     Document Type: Article

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