A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 30, 2012, Pages 12011-12016

  Pecqueur, Ludovic ^a   Duellberg, Christian ^b   Dreier, Birgit ^c   Jiang, Qiyang ^d   Wang, Chunguang ^d   Plückthun, Andreas ^c   Surrey, Thomas ^b   Gigant, Benoît ^a   Knossow, Marcel ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

^b IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^c UNIVERSITY OF ZURICH   (Switzerland)

^d TONGJI UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN; BINDING PROTEIN; DESIGNED ANKYRIN REPEAT PROTEIN; GUANOSINE TRIPHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; FLUORESCENCE MICROSCOPY; MICROTUBULE; MICROTUBULE ASSEMBLY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; TANDEM REPEAT; XENOPUS;

ANIMALS; ANKYRIN REPEAT; CRYSTALLOGRAPHY, X-RAY; DNA PRIMERS; FLUORESCENCE POLARIZATION; GUANOSINE TRIPHOSPHATE; MICROSCOPY, FLUORESCENCE; MICROTUBULES; MODELS, MOLECULAR; MULTIPROTEIN COMPLEXES; PROTEIN ENGINEERING; TUBULIN; XENOPUS;

EID: 84864343828     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1204129109     Document Type: Article

References (45)

1. Desai A, Mitchison TJ (1997) Microtubule polymerization dynamics. Annu Rev Cell Dev Biol 13:83-117. (Pubitemid 28034521)
2. Goodwin SS, Vale RD (2010) Patronin regulates the microtubule network by protecting microtubule minus ends. Cell 143:263-274.
3. Kollman JM, Merdes A, Mourey L, Agard DA (2011) Microtubule nucleation by γ-tubulin complexes. Nat Rev Mol Cell Biol 12:709-721.
4. Brouhard GJ, et al. (2008) XMAP215 is a processive microtubule polymerase. Cell 132:79-88.
5. Bringmann H, et al. (2004) A kinesin-like motor inhibits microtubule dynamic instability. Science 303:1519-1522. (Pubitemid 38314422)
6. Du Y, English CA, Ohi R (2010) The kinesin-8 Kif18A dampens microtubule plus-end dynamics. Curr Biol 20:374-380.
7. Silacci P, et al. (2004) Gelsolin superfamily proteins: Key regulators of cellular functions. Cell Mol Life Sci 61:2614-2623. (Pubitemid 39586521)
8. Wear MA, Cooper JA (2004) Capping protein: New insights into mechanism and regulation. Trends Biochem Sci 29:418-428. (Pubitemid 39061086)
9. Buey RM, Díaz JF, Andreu JM (2006) The nucleotide switch of tubulin and microtubule assembly: A polymerization-driven structural change. Biochemistry 45:5933-5938. (Pubitemid 43727084)
10. Nawrotek A, Knossow M, Gigant B (2011) The determinants that govern microtubule assembly from the atomic structure of GTP-tubulin. J Mol Biol 412:35-42.
11. Rice LM, Montabana EA, Agard DA (2008) The lattice as allosteric effector: Structural studies of αβ- and γ-tubulin clarify the role of GTP in microtubule assembly. Proc Natl Acad Sci USA 105:5378-5383. (Pubitemid 351753871)
12. Löwe J, Li H, Downing KH, Nogales E (2001) Refined structure of αβ-tubulin at 3.5 Å resolution. J Mol Biol 313:1045-1057. (Pubitemid 33081900)
13. Nogales E, Whittaker M, Milligan RA, Downing KH (1999) High-resolution model of the microtubule. Cell 96:79-88. (Pubitemid 29044155)
14. Ravelli RB, et al. (2004) Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain. Nature 428:198-202. (Pubitemid 38374318)
15. Helenius J, Brouhard G, Kalaidzidis Y, Diez S, Howard J (2006) The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. Nature 441:115-119.
16. Varga V, et al. (2006) Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol 8:957-962. (Pubitemid 44314726)
17. Binz HK, et al. (2004) High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol 22:575-582. (Pubitemid 38590636)
18. Dreier B, Plückthun A (2012) Rapid selection of high-affinity binders using ribosome display. Methods Mol Biol 805:261-286.
19. Hanes J, Plückthun A (1997) In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci USA 94:4937-4942. (Pubitemid 27214714)
20. Steiner D, Forrer P, Plückthun A (2008) Efficient selection of DARPins with subnanomolar affinities using SRP phage display. J Mol Biol 382:1211-1227.
21. Hall D, Minton AP (2005) Turbidity as a probe of tubulin polymerization kinetics: A theoretical and experimental re-examination. Anal Biochem 345:198-213. (Pubitemid 41375950)
22. Knipling L, Hwang J, Wolff J (1999) Preparation and properties of pure tubulin S. Cell Motil Cytoskeleton 43:63-71. (Pubitemid 29236876)
23. Fourniol FJ, et al. (2010) Template-free 13-protofilament microtubule-MAP assembly visualized at 8 Å resolution. J Cell Biol 191:463-470.
24. Dreier B, et al. (2011) Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. J Mol Biol 405:410-426.
25. Bieling P, Telley IA, Hentrich C, Piehler J, Surrey T (2010) Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and +TIP dynamics. Methods Cell Biol 95:555-580.
26. Bieling P, et al. (2007) Reconstitution of a microtubule plus-end tracking system invitro. Nature 450:1100-1105. (Pubitemid 350273611)
27. Walczak CE, Verma S, Mitchison TJ (1997) XCTK2: A kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts. J Cell Biol 136:859-870. (Pubitemid 27098742)
28. Scheel J, et al. (1999) Purification and analysis of authentic CLIP-170 and recombinant fragments. J Biol Chem 274:25883-25891.
29. Gupta ML, Jr., Carvalho P, Roof DM, Pellman D (2006) Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle. Nat Cell Biol 8:913-923. (Pubitemid 44314722)
30. Hunter AW, et al. (2003) The kinesin-related protein MCAK is a microtubule depolymerase that forms an ATP-hydrolyzing complex at microtubule ends. Mol Cell 11: 445-457. (Pubitemid 36293838)
31. Budde PP, Desai A, Heald R (2006) Analysis of microtubule polymerization in vitro and during the cell cycle in Xenopus egg extracts. Methods 38:29-34. (Pubitemid 41790294)
32. Wang HW, Nogales E (2005) Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature 435:911-915.
33. Honnappa S, Cutting B, Jahnke W, Seelig J, Steinmetz MO (2003) Thermodynamics of the Op18/stathmin-tubulin interaction. J Biol Chem 278:38926-38934. (Pubitemid 37221793)
34. Yamashita A, Maeda K, Maéda Y (2003) Crystal structure of CapZ: Structural basis for actin filament barbed end capping. EMBO J 22:1529-1538. (Pubitemid 36417402)
35. Kueh HY, Mitchison TJ (2009) Structural plasticity in actin and tubulin polymer dynamics. Science 325:960-963.
36. Weaver LN, et al. (2011) Kif18A uses a microtubule binding site in the tail for plus-end localization and spindle length regulation. Curr Biol 21:1500-1506.
37. Bieling P, Telley IA, Surrey T (2010) A minimal midzone protein module controls formation and length of antiparallel microtubule overlaps. Cell 142:420-432.
38. Walker RA, et al. (1988) Dynamic instability of individual microtubules analyzed by video light microscopy: Rate constants and transition frequencies. J Cell Biol 107:1437-1448.
39. Zahnd C, Sarkar CA, Plückthun A (2010) Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution. Protein Eng Des Sel 23:175-184.
40. Binz HK, Stumpp MT, Forrer P, Amstutz P, Plückthun A (2003) Designing repeat proteins: Well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol 332:489-503. (Pubitemid 37020959)
41. Wang W, et al. (2012) Kif2C minimal functional domain has unusual nucleotide binding properties that are adapted to microtubule depolymerization. J Biol Chem 287:15143-15153.
42. Adams PD, et al. (2010) PHENIX: A comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr 66:213-221.
43. Kramer MA, Wetzel SK, Plückthun A, Mittl PR, Grütter MG (2010) Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol 404:381-391.
44. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132. (Pubitemid 41742764)
45. Nogales E, Downing KH, Amos LA, Löwe J (1998) Tubulin and FtsZ form a distinct family of GTPases. Nat Struct Biol 5:451-458. (Pubitemid 28265028)