In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na+-citrate symporter CitS as an example

Journal of Structural Biology

Volumn 159, Issue 2 SPEC. ISS., 2007, Pages 206-221

  Huber, Thomas ^a   Steiner, Daniel ^a   Röthlisberger, Daniela ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Co crystallization; Designed ankyrin repeat proteins (DARPins); In vitro selection; Membrane protein; Multi angle (static) light scattering (MALS); Na+ citrate symporter CitS; Phage display; Protein engineering; Recombinant antibody Fab fragment; Ribosome display

Indexed keywords

BINDING PROTEIN; CITRIC ACID; COTRANSPORTER; DESIGNED ANKYRIN REPEAT PROTEIN; DETERGENT; EPITOPE; IMMUNOGLOBULIN F(AB) FRAGMENT; MEMBRANE PROTEIN; RECOMBINANT ANTIBODY; SODIUM; SODIUM CITRATE SYMPORTER; UNCLASSIFIED DRUG;

ANTIGEN RECOGNITION; ARTICLE; COMPARATIVE STUDY; CRYSTALLIZATION; HYBRIDOMA; IN VITRO SELECTION; KLEBSIELLA PNEUMONIAE; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; PROTEIN TARGETING; PROTEIN TERTIARY STRUCTURE; RIBOSOME; SOLUBILIZATION;

KLEBSIELLA PNEUMONIAE;

EID: 34447648701     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2007.01.013     Document Type: Article

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