Ochratoxin a: General overview and actual molecular status

Toxins

Volumn 2, Issue 4, 2010, Pages 461-493

  el Khoury, André ^a   Atoui, Ali ^b  

^a SAINT JOSEPH UNIVERSITY   (Lebanon)

^b NATIONAL COUNCIL FOR SCIENTIFIC RESEARCH   (Lebanon)

Author keywords

Biosynthesis; Detection; Molecular biology; Ochratoxin a; Polyketide synthase gene; Quantification; Toxicity

Indexed keywords

OCHRATOXIN;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ANALYTIC METHOD; ASPERGILLUS; CHEMICAL CARCINOGENESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOD ANALYSIS; FOOD CONTAMINATION; FOOD CONTROL; FUNGAL CONTAMINATION; FUNGAL DETECTION; FUNGAL METABOLISM; GENE CLUSTER; HUMAN; IMMUNOTOXICITY; LIQUID CHROMATOGRAPHY; MAXIMUM TOLERATED DOSE; MOLECULAR BIOLOGY; NEPHROTOXICITY; NEUROTOXICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PENICILLIUM; PHYSICAL CHEMISTRY; POLYMERASE CHAIN REACTION; RADIOIMMUNOASSAY; RANDOM AMPLIFIED POLYMORPHIC DNA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; TERATOGENICITY; THIN LAYER CHROMATOGRAPHY; TOXIN ANALYSIS; TOXIN STRUCTURE; TOXIN SYNTHESIS;

ABNORMALITIES, DRUG-INDUCED; AMINO ACID SEQUENCE; ANIMALS; FOOD CONTAMINATION; FUNGI; HUMANS; IMMUNE SYSTEM; KIDNEY; MOLECULAR SEQUENCE DATA; MYCOTOXINS; NEOPLASMS; OCHRATOXINS; POLYMERASE CHAIN REACTION;

ASPERGILLUS; FUNGI; PENICILLIUM;

EID: 78649357553     PISSN: None     EISSN: 20726651     Source Type: Journal    
DOI: 10.3390/toxins2040461     Document Type: Review

References (206)

1. Keeper-Goodman, T.; Scott, P.M. Risk assessment of the mycotoxin ochratoxin. Biomed. Environ. Sci. 1989, 2, 179-248.
2. Miller, J.D.; Trenholm, H.L. Mycotoxins in Grain: Compounds Other than Aflatoxin; Eagan Press: St. Paul, MN, USA, 1994.
3. Bredenkamp, M.W.; Dillen, J.L.M.; Van Rooyen, P.H.; Steyn, P.S. Crystal structures and conformational analysis of ochratoxin A and B: Probing the chemical structure causing toxicity. J. Chem. Soc. 1989, 2, 1835-1839.
4. Budavari, S. The Merck Index, 11th ed.; Rahway, N.J., Ed.; Merck & Co.: Rahway, NJ, USA, 1989; pp. 30-31.
5. Faucet-Marquis, V.; Pont, F.; Størmer, F.C.; Rizk, T.; Castegnaro, M.; Pfohl Leszkowicz, A. Evidence of a new dechlorinated ochratoxin A derivative formed in opossum kidney cell cultures after pretreatment by modulators of glutathione pathways: Correlation with DNA-adduct formation. Mol. Nurt. Food Res. 2006, 50, 530-542.
6. Tozlovanu, M.; Faucet-Marquis, V.; Pfohl-Leszkowicz, A.; Manderville, R. Genotoxicity of the hydroquinone metabolite of ochratoxin A: Structure-activity relationships for covalent DNA adduction. Chem. Res. Toxicol. 2006, 19, 1241-1247
7. Pfohl-Leszkowicz, A.; Manderville, R.A. Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans. Mol. Nutr. Food Res. 2007, 51, 61-99.
8. Manderville, R.A.; Pfohl-Leszkowicz, A. Bioactivation and DNA adduction as a rationale for ochratoxin A carcinogenesis. World Mycotoxin J. 2008, 1, 357-367
9. Miller, J.D. Fungi as contaminants in indoor air. Atmos. Environ. 1992, 26, 2163-2172.
10. Steyn, P.S. Ochratoxin and related dihydroisocoumarins. In Mycotoxin-Production, Isolation, Separation and Purification; Betina, V., Ed.; Elseiver: Amsterdam, The Netherlands, 1984; pp. 183-216
11. de Jesus, A.E.; Steyn, P.S.; Vleggaar, R.; Wessels, P.A. Carbon-13 nuclear magnetic resonance assignments and biosynthesis of the mycotoxin ochratoxin A. J. Chem. Soc. 1980, 1, 52-54.
12. Pohland, A.E.; Schüller, P.L.; Steyn, P.S.; van Egmond, H. Physico-chemical data for selected mycotoxins. Pure Appl. Chem. 1982, 54, 2219-2284.
13. EL Khoury, A. Champignons Mycotoxinogènes et Ochratoxine A (OTA) et Aflatoxine B1 (AFB1) dans les Vignobles Libanais: Occurrence et Origine; Ph.D. Thesis, INP: Toulouse, France, 2007.
14. Lillehoj, E.B.; Goransson, B. Occurrence of ochratoxin and citrinin producing fungi on developing Danish barley grain. Acta Pathol. Microbiol. Scand. B 1980, 88, 133-137
15. Abramson, D. Measurement of ochratoxin A in barely extracts by liquid chromatography mass spectrometry. J. Chromatogr. 1987, 391, 315-320.
16. Bauer, J.; Gareis, M. Ochratoxin A im der Nahrungsmittelkette. J. Vet. Med. Ser. B. 1987, 34, 613.
17. Müller, H.M. Decontamination of mycotoxins. I. Physical process. Ubersicht. Tierernähr. 1982, 10, 95-122.
18. Trivedi, A.B.; Doi, E.; Kitabatake, N. Detoxification of ochratoxin A on heating under acidic and alkaline conditions. Biosci. Biotech. Biochem. 1992, 56, 741-745.
19. Boudra, H.; Le Bars, P.; Le Bars, J. Thermostability of ochratoxin A in wheat under two moisture conditions. Appl. Environ. Microbiol. 1995, 61, 1156-1158.
20. Castegnaro, M.; Barek, J.; Frémy, J.M.; Lafontaine, M.; Miraglia, M.; Sansone, E.B.; Telling, G.M. Laboratory Decontamination and Destruction of Carcinogens in Laboratory Wastes: Some Mycotoxins; International Agency for Research on Cancer: Lyon, France, 1991.
21. IARC. Some naturally occurring substances, food items and constituents, heterocyclic aromatic amines and mycotoxins. In Monograph on evaluation of carcinogenic risks to humans; IARC: Lyon, France, 1993.
22. Zimmerli, B.; Dick R. Ochratoxin A in table wine and grape-juice: Occurrence and risk assessment. Food Addit. Contam. 1996, 13, 655-668.
23. Delage, N.; d'Harlingue, A.; Colonna, B.; Ceccaldi, B.C.; Bompeix, G. Occurrence of mycotoxins in fruit juices and wines. Food Control 2003, 14, 225-227.
24. Da Rocha Rosa, C.A.; Palacios, V.; Combina, M.; Fraga, M.E.; De Oliveira Rekson, A.; Magnoli, C.E.; Dalcero, A.M. Potential ochratoxin A producers from wine grapes in Argentina and Brazil. Food Addit. Contam. 2002, 19, 408-414.
25. Shephard, G.S.; Fabiani, A.; Stockenstrom, S.; Mshicileli, N.; Sewram, V. Quantitation of ochratoxin A in South African wines. J. Agric. Food. Chem. 2003, 51, 1102-1106.
26. Visconti, A.; Pascale, M.; Centoze, G. Determination of ocratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography. J. Chromatogr. A 1999, 864, 89-101.
27. Ospital, M.; Cazabeil, J.M.; Betbede, A.M.; Tricard, C.; Creppy, E.; Medina, B. L'Ochratoxine A dans les vins. OEnologie 1998, 169, 16-19.
28. Otteneder, H.; Majerus, P. Occurence of ochratoin A (OTA) in wines: Influence of the type of wine and its geographical origin. Food Addit. Contam. 2000, 17, 793-798.
29. O'Brien, E.; Heussner, A.H.; Dietrich, D.R. Species-, sex-, and cell type-specific effects of ochratoxin A and B. Toxicol Sci. 2001, 63, 256-264
30. Faucet, V.; Pfohl-Leszkowicz, A.; Dai, J.; Castegnaro, M.; Manderville, R.A. Evidence for covalent DNA adduction by Ochratoxin A following chronic exposure to rat and subacute exposure to pig. Chem. Res. Toxicol. 2004, 17, 1289-1296.
31. Ribelin, W.E.; Fukushima, K.; Still, P.E. The toxicity of ochratoxin A to ruminants. Can. J. Comp. Med. 1978, 42, 172-176.
32. Elling, F.; Nelsen, J.P.; Lillehoj, E.B.; Thomassen, M.S.; Stormer, F.C. Ochratoxin A induced porcine nephropathy: Enzyme and ultrastructural changes after short-term exposure. Toxicon 1985, 23, 247-254.
33. Lopez de Cerain, A.; Gonzalez-Penas, E.; Jimenez, A.M.; Bello, J. Contribution to the study of Ochratoxin A in Spanish wines. Food Addit. Contam. 2002, 19, 1058-1064.
34. Bejaoui, H. Champignons Ochratoxinogènes et Ochratoxine A (OTA) dans des Vignobles Français et procEdés Biologiques de décontamination de l'OTA dans les moûts de raisin; Ph.D. Thesis, INP: Toulouse, France, 2005.
35. Pfohl-Leszkowicz, A. Ochratoxin A and aristolochic acid involvement in nephropathies and associated urothelial tract tumours. Arh. Hig. Rada. Toksikol. 2009, 60, 465-483.
36. Markaki, P.; Delpont, C.; Delpont-Binet, C.; Grosso, F.; Dragacci, S. Determination of ochratoxin A in red wine and vinegar bu immunoaffinity high-pressure liquid chromatography. J. Food Prot. 2001, 64, 533-537.
37. Hassen, W.; Abid-Essafi, S.; Achour, A.; Guezzah, N.; Zakhama, A.; Ellouz, F.; Creppy, E.E.; Bacha, H. Karyomegaly of tubular kidney cells in human chronic interstitial nephropathy in Tunisia: Respective role of Ochratoxin A and possible genetic predisposition. Hum. Exp. Toxicol. 2004, 23, 339-346
38. Pfohl-Leszkowicz, A.; Castegnaro, M. L'Ochratoxine A dans: Mycotoxines: Evaluation et gestion du risqué, Lavoisier; Tec & Doc: Paris, France, 1999; pp. 249-278.
39. Soleas, G.J.; Yan, J.; Goldberg, D.M. Assay of ochratoxin A in wine and beer by high-pressure liquid chromatography photodiode array and gas chromatography mass selective detection. J. Agric. Food Chem. 2001, 49, 2733-2740.
40. Brown, M.H.; Szczech, G.M.; Purmalis, B.P. Teratogenic and toxic effects of ochratoxin A in rats. Toxicol. Appl. Pharmacol. 1976, 37, 331-338
41. Mayura, K.; Reddy, R.V.; Hayes, A.W.; Berndt, W.O. Embryocidal, fetotoxic and teratogenic effects of ochratoxin A in rats. Toxicology 1982, 25, 175-185
42. Mayura, K.; Parker, R.; Berndt, W.O.; Philips, T.D. Effect of simultaneous prenatal exposure to ochratoxin A and citrinin in the rat. Toxicol. Environ. Health 1984, 13, 553-561
43. Mayura, K.; Stein, A.E.; Berndt, W.O.; Philips, T.D. Teratogenic effects of ochratoxin A in rats with impaired renal function. Toxicology 1984, 32, 277-285
44. Mayura, K.; Parker, R.; Berndt, W.O.; Philips, T.D. Ochratoxin A-induced teratogenesis in rats: Partial protection by phenylalanine. Appl. Environ. Microbiol. 1984, 48, 1186-1188
45. Hayes, A.W.; Melton, R.; Smith, S.J. Effect of aflatoxin B, ochratoxin A and rubratoxin B on a protozoan, Tetrahymena pyriformis HSM. Bull. Environ. Contamin. Toxicol. 1974, 11, 321-325.
46. Hood, R.D., Naughton, M.J.; Hayes, A.W. Prenatal effects of ochratoxin A in hamsters. Teratology 1976, 13, 11-14.
47. Gilani, S.H.; Bancroft J.; Reily M. Teratogenicity of ochratoxin A in chick embryos. Toxicol. Appl. Pharmacol. 1978, 46, 543-546.
48. More, J.; Galtier, P.; Alvinerie, M. Toxicite de l'ochratoxine A. III. Effets pendant les stades initiaux de la gestation chez le rat. Ann. Rech. Vet. 1978, 9, 169-173.
49. Petzinger, E.; Weidenbach, A. Mycotoxins in the food chain: The role of ochratoxins. Livest.Prod. Sci. 2002, 76, 245-250.
50. Creppy, E.E.; Betbeder, A.M.; Gharbi, A.; Counord, J.; Castegnaro, M.; Bartsch, H.; Moncharmont, P.; Fouillet, B.; Chambon, P.; Dirheimer, G. Human ochratoxicosis in France. In Mycotoxins, Endemic Nephropathy and Urinary Tract Tumours; Castegnaro, M., Plestina, R., Dirheimer, G., Chernozemsky, I.N., Bartsch, H., Eds.; IARC Scientific Publ.: Lyon, France, 1991.
51. Holmberg, T.; Thuvander, A.; Hult, K. Ochratoxin A as a suppressor of mitogen-induced blastogenesis of porcine blood lymphocytes. Acta Vet. Scand. 1988, 29, 219-223.
52. Lea, T.; Steien K.; Stormer C. Mechanism of ochratoxin A induced immunosuppresion. Mycopathologia 1989, 107, 153-159.
53. Huff, W.E.; Kubena, L.F.; Harvey, R.B.; Phillips, T.D. Efficacy of hydrated sodium calcium aluminosilicate to reduce the individual and combined toxicity of aflatoxin and ochratoxin A. Poult Sci. 1992, 71, 64-69
54. Pfohl-Leszkowicz, A.; Pinelli, E.; Bartsch, H.; Mohr, U.; Castegnaro, M. Sex- and strain-specific expression of cytochrome P450s in ochratoxin A-induced genotoxicity and carcinogenicity in rats. Mol. Carcinog. 1998, 23, 76-85.
55. NTP. Toxicology and Carcinogenesis Studies of Ochratoxin A (CAS No. 303-47-9) in F344/N Rats (Gavage Studies). Technical Report Series No 358; NTIS Publication No. PB90-219478/AS; National Toxicology Program: Research Triangle Park, NC and Bethesda, MD, USA, 1989.
56. Feier, D.; Tofana, M. Ochratoxin A-Toxicological Aspects. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture 2009, 66, 308-312.
57. Van der Merve, K.J.; Steyn, P.S.; Fourie, L.; Scott, D.B.; Theron, J.J. Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus. Nature 1965, 205, 1112-1113.
58. Varga, J.; Rigó, K.; Téren, J.; Mesterházy, Á. Recent advances in ochratoxin research I. Production, detection and occurrence of ochratoxins. Cereal Res. Commun. 2001, 29, 85-92.
59. Frisvad, J.C.J.; Frank, M.; Houbraken, J.A.M.P.; Kuijpers, A.F.A.; Samson, R.A. New ochratoxin A producing species of Aspergillus section Circumdati. Stud. Mycol. 2004, 50, 23-43.
60. Horie, Y. Productivity of ochratoxin A of Aspergillus carbonarius in Aspergillus section Nigri. Nippon Kingakukai Kaiho 1995, 36, 73-76.
61. IARC. IARC Handbooks of Cancer Prevention. Weight Control and Physical Activity; International Agency for Research on Cancer: Lyon, France, 2002.
62. Leong, S.; Hocking, A.D.; Pitt, J.I. Occurrence of fruit rot fungi (Aspergillus section Nigri) on some drying varieties of irrigated grapes. Aust. J. Grape Wine Res. 2004, 10, 83-88.
63. Taniwaki, M.H.; Pitt, J.I.; Teixeira, A.A.; Iamanaka, B.T. The source of ochratoxin A in Brazilian coffee and its formation in relation to processing methods. Int. J. Food Microbiol. 2003, 82, 173-179.
64. Abarca, L.M.; Accensi, F.; Cano, J.; Cabanes, J.F. Taxonomy of black aspergilli. Ant. Van Leeuwen. 2004, 86, 33-49.
65. Dalcero, A.; Magnoli, C.; Hallak, C.; Chiacchiera, S.M.; Palacio, G.; Rosa, C.A. Detection of ochratoxin A in animal feeds and capacity to produce this mycotoxin by Aspergillus section Nigri in Argentina. Food Addit. Contam. 2002, 19, 1065-1072.
66. Magnoli, C.; Violante, M.; Combina, M.; Palacio G.; Dalcero, A. Mycoflora and ochratoxinproducing strains of Aspergillus section Nigri in wine grapes in Argentina. Lett. Appl. Microbiol. 2003, 37, 179-184.
67. Battilani, P.; Giorni, P.; Pietri, A. Epidemiology of toxin producing fungi and ochratoxin A occurrence in grape. Eur. J. Plant Pathol. 2003, 109, 715-722.
68. Samson, R.A.; Hoekstra, E.S.; Frisvad, J.C. Introduction to Food and Airborne Fungi, 7th ed.; Centraalbureau voor Schimmelcultures: Utrecht, The Netherlands, 2004.
69. Abarca, M.L.; Accensi, F.; Bragulat, M.R.; Cabanes, F.J. Current importance of ochratoxin A producing Aspergillus spp. J. Food Protec. 2001, 903-906.
70. Ciegler, A. Bioproduction of ochratoxin A and penicillc acid by members of the Aspergillus ochraceus group. Can. J. Microbiol. 1972, 18,631-636
71. Hesseltine, C.W.; Vandegraft, E.E.; Fennell, D.I.; Smith, M.L.; Shotwell, O.L. Aspergilli as ochratoxin producers. Mycologia 1972, 64, 539-550.
72. Frisvad, J.C. Creatine sucrose agar, a differential medium for mycotoxin producing terverticillate Penicillium species. Lett. Appl. Microbiol. 1985, 1, 109-113.
73. Geisen, R.; Mayer, Z.; Karolewiez, A.; Farber, P. Development of a real time PCR system for detection of Penicillium nordicum and for monitoring ochratoxin A production in foods by targeting the ochratoxin polyketide synthase gene. Syst. Appl. Microbiol. 2004, 27, 501-504.
74. Pitt, J.I. Penicillium viridicatum, Penicillium verrucosum, and production of ochratoxin A. Appl. Environ. Microbiol. 1987, 53, 266-269.
75. Lund, F.; Frisvad, J.C. Penicillium verrucosum in cereals indicates production of ochratoxin A. J. Appl. Microbiol. 2003, 95, 1117-1123.
76. Larsen, T.O.; Svendsen, A.; Smedsgaard, J. Biochemical characterization of ochratoxin Aproducing strains of the genus Penicillium. Appl. Environ. Microbiol. 2001, 67, 3630-3635.
77. Eskola, M. Study on Trichothecenes, Zearalenone and Ochratoxin A in finnish Cereals: Occurrence and Analytical Techniques; Academic dissertation: Skillman, NJ, USA, 2002.
78. FAO. Agro-ecological assessment for national planning: The example of Kenya. FAO soils bulletin No 67. Roma, Italy, 1993.
79. FAO/WHO. Ochratoxin A. In Safety evaluation of certain mycotoxins in food, Prepared by the 56th Meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA). WHO Food Additives Series 47; World Health Organisation: Geneva, Switzerland, 2001; pp. 281-387.
80. Pittet, A. Natural occurrence of mycotoxins in foods and feeds an updated review. Rev. Med. Vet. 1998, 149, 479-492.
81. Nguyen, M.T.; Tozlovanu, M.; Tran, T.L.; Pfohl-Leszkowicz, A. Occurrence of afl atoxin B1, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam. Food Chem. 2007, 105, 42-47.
82. Vega, M.; Muñoz, K.; Sepúlveda, C.; Aranda, M.; Campos, V.; Villegas, R., Villarroel, O. Solidphase extraction and HPLC determination of ochratoxin A in cereals products on Chilean market. Food Control 2009, 20, 631-634
83. Otteneder, H.; Majerus, P. Occurrence of ochratoxin A in wines: Influence of the type of wine and its geographical origin. Food Addit. Contam. 2000, 17, 793-798.
84. Cabañes, F.J.; Accensi, F.; Bragulat, M.R.; Abarca, M.L.; Castella, G.; Minguez, S.; Pons, A. What is the source of Ochratoxin A in wine? Int. J. Food Microbiol. 2002, 79, 213-215.
85. Stefanaki, I.; Foufa, E.; Tsatsou-Dritsa, A.; Dais, P. Ochratoxin A concentrations in Greek domestic wines and dried vine fruits. Food Addit. Contam. 2003, 20, 74-83.
86. Shephard, G.S.; Fabiani, A.; Stockenstrom, S.; Mshicileli, N.; Sewram, V. Quantitation of ochratoxin A in south African wines. J. Agri. Food Chem. 2003, 51, 1102-1106.
87. Castellari, M.; Fabbr, S.; Fabiani, A.; Amati, A.; Galassi, S. Comparison of different immunoaffinity clean-up procedures for high-performance liquid chromatographic analysis of ochratoxin A in wines. J. Chromatogr. A 2000, 888, 129-136.
88. Serra, R.; Abrunhosa, L.; Kozakiewicz, Z.; Venâncio, A. Black Aspergillus species as ochratoxin A producers in Portuguese wine grapes. Int. J. Food Microbiol. 2003, 88, 63-68.
89. Bejaoui, H.; Mathieu, F.; Taillandier, P.; Lebrihi A. Biodegradation of ochratoxin A by Aspergillus section Nigri species isolated from French grapes: A potential means of ochratoxin A decontamination in grape juices and musts. FEMS Microbiol. Lett. 2006, 255,203-208.
90. EL Khoury, A.; Rizk, T.; Lteif, R.; Azouri, H.; Delia, M.L.; Lebrihi, A. Occurrence of ochratoxin A and aflatoxin B1-producing fungi in Lebanese grapes and OTA content in musts and finished wines during year 2004. J. Agri. Food Chem. 2006, 54, 8977-8982.
91. Wolff, J.; Bresch, H.; Cholmakov-Bodechtel, C.; Engel, G.; Garais, M.; Majerus, P.; Rosner, H.; Scheuer, R. Ochratoxin A: Contamination of foods and consumer exposure. Arch Lebensm hyg. 2000, 51, 181-128.
92. FAO. Aflatoxin Contamination in Foods and Feeds in the Philippines. Document 13. FAO/WHO Regional Conference on Food Safety for Asia and Pacific Seremban, Malaysia, 2004.
93. Hussein, H.S.; Brasel, J.M. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology 2001, 167, 101-134.
94. Van Egmond, H.P.; Svensson, U.K.; Fremy, J.M. Mycotoxins. Int. Dairy Fed. S.I. 1997, 79-88.
95. Commission Regulation (EC) No 466/2001 of 8 March 2001 setting maximum levels for certain contaminants in foodstuffs. Off. J. Eur. Commun. 2001, L77-L113.
96. European Commission. Commission regulation (EC) No 2003/100/EC of 31 October 2003 amending Annex I to Directive 2002/32/EC of the European Parliament and of the Council on undesirable substances in animal feed. Off. J. Eur. Commun. 2003, 285, 33-37.
97. European Commission. Commission Regulation (EC) No 2004/683/EC of 13 April 2004 amending Regulation (EC) No 466/2001 as regards aflatoxins and ochratoxin A in foods for infants and young children (Text with EEA relevance). Off. J. Eur. Commun. 2004, 106, 3-5.
98. European Commission. 2003. Commission Regulation (EC) No 2003/2174/EC of 12 december 2003 amending Regulation 466/2001/EC as regards aflatoxins. Off. J. Eur. Commun. 2003, 326, 12.
99. Van Egmond, H.P.; Schothorst, R.C.; Jonker, M.A. Regulations relating to mycotoxins in food: Perspectives in a global and European context. J. Anal. Bioanal. Chem. 2007, 389, 147-157
100. WHO. Evaluation of certain food additives and contaminants. Thirty-seventh report of the joint FAO/WHO Expert Committee on Food Additives. WHO technical report series 806; World Health Organization: Geneva, Switzerland, 1991; pp. 29-31.
101. FAO. Biotechnology and food safety. Report of a Joint FAO/WHO consultation. Food and Agricultural Organization, Food and Nutrition, 1996.
102. FAO. Joint FAO/WHO Expert Committee on Food Additives, Fifty-sixth meeting, Geneva, Switzerland, 2001; pp. 6-15.
103. EC European Community. Opinion on of the Scientific Committee on Food (SCF) on Ochratoxin A, Luxembourg, 1998. Expressed on 17 September.
104. European Commission. Commission Regulation (EC) No 472/2002 of 12 March 2002. Off. J. Eur. Commun. 2002, 75, 18-20.
105. European Commission. Commission Regulation (EC) No 466/2001 of 8 March 2001. Off. J. Eur. Commun. No. 2001, 77, 1-13.
106. European Commission. Commission Regulation (EC) No 123/2005 of 26 January 2005 amending Regulation (EC) No 466/2001 as regards ochratoxin A. Off. J. Eur. Commun. 2005, 25, 3-5.
107. Senyuva, H.Z.; Gilbert, J.; Ozcan, S.; Ulken, U. Survey of ochratoxin A and afaltoxin B1 in dried figs in Turkey using a single laboratory validated alkaline extraction method for ochratoxin A. J. Food Protect. 2005, 68, 1512-1515.
108. Levi, C.; Trenk, H.L.; Mohr, H.K. Study of the occurrence of ochratoxin A in green coffee beans. J. Assoc. Offic. Anal. Chem. 1974, 57, 866-870.
109. Nesheim, S.; Hardin, N.F.; Francis, O.I.; Langham, W.S. Analysis of ochratoxin A and B and their esters in barley: Using partitions and thin-layer chromatography. I Development of the method. J. AOAC Int. 1973, 56, 817-821.
110. El Adlouni, C.; Tozlovanu, M.; Naman, F.; Faid, M.; Pfohl-Leszkowicz, A. Preliminary data on the presence of mycotoxins (Ochratoxin A, citrinin and aflatoxin B1) in black table olives" Greek style" of Moroccan origin. Mol. Nutr. Food Res. 2006, 50, 507-512.
111. Mac Donald, S.; Wilson, P.; Barnes, K.; Damant, A.; Massey, R.; Mortby, E.; Shephered, M.J. Ochratoxin A in dried vine fruit: method development and survey. Food Addit Contam. 1999, 16, 253-260.
112. Legarda, T.M., Burdaspal, P.A. Ochratoxin A in beers brewed in Spain and other European countries. Alimentaria 1998, 291, 115-122.
113. NF EN 14133:2003. Food products. Determination of ochratoxin A in wine and beer - HPLC method with immunoaffinity column clean-up. Dansk Standard; Classification index V 03-128. IHS: Englewood, CO, USA, 2003.
114. Leoni, L.A.B.; Valente Soares, L.M.; Oliveira, P.L.C. Ochratoxin A in Brazilian roasted and instant coffees. Food Addit. Contam. 2000, 17, 10, 867-870.
115. Stegen, V.D.G.; Jorissen, U.; Pittet, A.; Saccon, M.; Steiner, W.; Vincenzi, M.; Winkler, M.; Zapp, J.; Schlatter, C. Screening of European coffee final products for occurrence of ochratoxin A (OTA). Food Addit. Contam. 1997, 14, 211-216.
116. Valenta, H.; Goll M. Determination of ochratoxin A in regional samples of cow's milk from Germany. Food Addit. Contam. 1996, 13, 669-676.
117. Entwistle, C.A.; Williams, A.C.; Mann, P.J.; Russell, J.; Slack, P.; Gilbert, J. Combined phenyl silane and immunoaffinity column cleanup with liquid chromatography for determination of ochratoxin A in roasted coffee; Collaborative study. J. Assoc. Offic. Anal. Chem. Int. 2001, 84, 444-450.
118. Gilbert, J.; Brereton, P.; MacDonald, S. Assessment of dietary exposure to ochratoxin A in the UK using a duplicate diet approach and analysis of urine and plasma samples. Food Addit. Contam. 2001, 18, 1088-1093.
119. Entwistle, A.C.; Williams A.C.; Mann P.J.; Stack P.T.; Gilbert J. Liquid chromatographic method with immunoaffinity column clean up for determination of ochratoxin A in barley: Collaborative study. J. Assoc. Anal. Comm. 2000, 83, 1377-1383
120. Entwistle, A.C.; Williams, A.C.; Mann, P.J.; Russell, J.; Stack, P.T.; Gilbert, J. Combined phenyl silane and immunoaffinity column clean up with liquid chromatography for determination of ochratoxin A in roasted coffee: Collaborative study. J. Assoc. Anal. Comm. 2001, 84, 444-450.
121. Burdaspal, P.; Legarda, T.M.; Gilbert, J. Determination of ochratoxin A in baby food by immunoaffinity column cleanup with liquid chromatography: Interlaboratory study. J. Assoc. Offic. Anal. Chem. Int. 2001, 84, 1445-1452.
122. Dragacci, S.; Frémy, J.M. Dosage des mycotoxines. In Moisissures des aliments peuhydratés; Cahagnier, B., Ed.; Tec et Doc: Paris, France, 1998; pp. 187-207.
123. Wilson, D.M.; Sydenham, E.W.; Lombaert, G.A.; Trucksess, M.W.; Abramson, D.; Bennett, G.A. Mycotoxin analytical techniques. In Mycotoxins in Agriculture and Food Safety; Sinha, K.K., Bhatnagar, D., Eds.; Marcel Dekker, Inc.: New York, NY, USA, 1998.
124. Fukal, L.; Reisnerova, H. Monitoring of aflatoxins and ochratoxin A in Czechoslovak human sera by immunoassay. Bull. Environ. Contam. Toxicol. 1990, 44, 345-349.
125. Fukal, L.; Marek, M. A screening method for simultaneous immunochemical detection of aflatoxins and ochratoxin A in cereals and feed. Vet. Med. 1991, 36, 251-254
126. Rousseau, D.M.; Candlish, A.G.; Slegers, G.A.; Van Peteghem, C.H.; Stimson, W.H.; Smith, J.E. Detection of ochratoxin A in porcine kidneys by a monoclonal antibody-based radioimmunoassay. Appl. Environ. Microbiol. 1987, 53, 514-518.
127. Aizawa, M. Immunosensors. Philos. T. R. Soc. B 1987, 316, 121-134.
128. Niessen, L.; Schmidt, H.; Muhlencoert, E.; Farber, P.; Karolewiez, A., Geisen, P. Advances in the molecular diagnosis of ochratoxin A-producing fungi. Food Addit. Contam. 2005, 22, 324-334
129. Edwards, S.G.; O'Callaghan, J.; Dobson, A.D.W. PCR-based detection and quantification of mycotoxigenic fungi. Mycol. Res. 2002, 106, 1005-1025.
130. Moss, M.O. Recent studies of mycotoxins. Symp. Ser. Soc. Appl. Microbiol. 1998, 27, 62S-76S.
131. Proctor, R.H.; Desjardins, A.E.; Plattner, R.D.; Hohn, T.M. A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal Genet. Biol. 1999, 27, 100-112.
132. Bhatnagar, D.; Ehrlich, K.C.; Cleveland, T.E. Molecular genetic analysis and regulation of aflatoxin biosynthesis. Appl. Microbiol. Biotechnol. 2003, 61, 83-93.
133. Varga, J.; Kevei, E.; Rinyu, E.; Teren, J.; Kozakiewicz, Z. Ochratoxin production by Aspergillus species. Appl. Environ. Microbiol. 1996, 62, 4461-4464.
134. Huff, W.E.; Hamilton, P.B. Mycotoxins-their biosynthesis in fungi: Ochratoxins-metabolites of combined pathways. J. Food Prot. 1979, 42, 815-820.
135. Schulz, B.; Sucker, J.; Aust, H.J.; Krohn, K.; Ludewig, K.; Jones, P.G. and Doring, D. Biologically active secondary metabolites of endophytic Pezicula species. Mycol. Res. 1995, 99, 1007-1015.
136. Venkatasubbaiah, P.; Sutton, T.B.; and Chilton, W.S. Effect of phytotoxins produced by Botryosphaeria obtusa, the cause of black rot of apple fruit and frogeye leaf spot. Physiol. Biochem. 1991, 81, 243-247.
137. Keller, B.; Winzeler, H.; Winzeler, M. and Fried, P.M. Differential sensitivity of wheat embryos against extracts containing toxins of Septoria nodorum: First Steps towards in vitro selection. J. Phytopathol. 1994, 141, 233-240.
138. Parisi, A.; Piattelli, M.; Tringali, C.; San Lio G.M.D.; Di San Lio, G.M. Identification of the phytotoxin mellein in culture fluids of Phoma tracheiphila; Phytochemistry: Oxford, UK, 1993; pp. 865-867.
139. Turner, W.B., Albridge, D.C. Fungal Metabolites; Academic Press Inc.: London, UK, 1983.
140. Camarda, L.; Merlini, L.; Nasini, G. Metabolites of Cercospora. Taiwapyrone, an α-pyrone of unusual structure from Cercospora Taiwanensis. Phytochemistry 1976, 15, 537-539.
141. Assante, G.; Locci, R.; Camarda, L.; Merlini, L. and Nasini, G. Screening of the genus Cercospora for secondary metabolites. Phytochemistry 1977, 16, 243-247.
142. Claydon, N.; Grove, J.F.; Pople, M. Insecticidal secondary metabolic products from the entomogenous fungus Fusarium larvarum. J. Invert. Pathol. 1979, 33,364-367
143. Krohn, K.; Bahramsari, R.; Florke, U.; Ludewig, K.; Kliche-Spory, C.; Michel, A.; Aust, H.J.; Draeger, S.; Schulz, B.; Antus, S. Dihydroisocoumarins from fungi: Isolation, structure elucidation, circular dichroism and biological activity. Phytochemistry 1997, 45, 313-320.
144. Harris, J.P.; Mantle, P.G. Biosynthesis of diaporthin and orthosporin by Aspergillus ochraceus. Phytochemistry 2001, 57, 165-169.
145. Bingle, L.E.; Simpson, T.J.; Lazarus, C.M. Ketosynthase domain probes identify two subclasses of fungal polyketide synthase genes. Fungal Genet. Biol. 1999, 26, 209-223.
146. Liou, G.F.; Khosla, C. Building-block selectivity of polyketide synthases. Curr. Opin. Chem. Biol. 2003, 7, 1-6.
147. Nicholson, T.P.; Rudd, B.A.M.; Dawson, M.; Lazarus, C.M.; Simpson, T.J.; Cox, R.J. Design and utility of oligonucleotide gene probes for fungal polyketide synthases. Chem. Biol. 2001, 8, 157-178.
148. O'Callaghan, J.; Caddick, M.X.; Dobson, A.D.W. A polyketide synthase gene required for ochratoxin A biosynthesis in Aspergillus ochraceus. Microbiology 2003, 149, 3485-3491.
149. Varga, J.; Rigó, K.; Kocsubé, S.; Farkas, B.; Pál, K. Diversity of polyketide synthase gene sequences in Aspergillus species. Res. Microbiol. 2003, 154, 593-600.
150. Atoui, A.; Dao, P.; Mathieu, F.; Lebrihi, A. Amplification and diversity analysis of Ketosynthase domains of putative polyketide synthase gene in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A. Mol. Nutr. Food Res. 2006, 50, 448-493.
151. Karolewiez, A.; Geisen, R. Cloning a part of the ochratoxin A biosynthetic gene cluster of Penicillium nordicum and characterisation of the ochratoxin polyketide synthase gene. Syst. Appl. Microbiol. 2005, 28, 588-595.
152. Bacha, N.; Atoui, A.; Mathieu, F.; Liboz, T.; Lebrihi A. Aspergillus westerdijkiae polyketide synthase gene aoks1 is involved in the biosynthesis of ochratoxin A. Fungal Genet. Biol. 2009, 46, 77-84.
153. Sidhu, G.S. Mycotoxin genetics and gene clusters. Eur. J. Plant Pathol. 2002, 108, 705-711.
154. Yu, J.; Keller, N. Regulation of secondary metabolism in filamentous fungi. Annu. Rev. Phytopathol. 2005, 43, 437-458.
155. Brown, D.W.; Yu, J.H.; Kelkar, H.S.; Fernandes, M.; Nesbitt, T.C.; Keller, N.P.; Adams, T.H.; Leonard, T.J. Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. Proc. Natl. Acad. Sci. USA 1996, 93, 1418-1422.
156. Kato, N.; Brooks, W.; Calvo, A.M. The expression of sterigmatocystin and penicillin genes in Aspergillus nidulans is controlled by veA, a gene required for sexual development. Eukaryot Cell 2003, 2, 1178-1186.
157. Yu, J.; Chang, P.K.; Ehrlich, K.C.; Cary, J.W.; Bhatnagar, D.; Cleveland, T.E.; Payne, G.A.; Linz, J.E.; Woloshuk, C.P.; Bennett, J.W. Clustered pathway genes in aflatoxin biosynthesis. Appl. Environ. Microbiol. 2004, 70, 1253-1262
158. O'Callaghan, J.; Stapleton, P.C; Dobson, A.D.W. Ochratoxin A biosynthetic genes in Aspergillus ochraceus are differentially regulated by pH and nutritional stimuli. Fungal Genet. Biol. 2006, 43, 213-221.
159. Yu, J.; Chang, P.-K.; Cary, J.W.; Bhatnagar, D.; Cleveland, T.E. AvnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus. Appl. Environ. Microbiol. 1997, 63, 1349-1356.
160. Ward, T.J.; Bielawski, J.P.; Kistler, H.C.; Sullivan, E.; O'Donnell, K. Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic Fusarium. Proc. Natl. Acad. Sci. USA 2002, 99, 9278-9283.
161. Ehrlich, K.C.; Chang, P.-K.; Yu, J.; Cotty, P.J. Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation. Appl. Environ. Microbiol. 2004, 70, 6518-6524.
162. Dao, H.P.; Mathieu, F.; Lebrihi, A. Two primer pairs to detect OTA producers by PCR method. Int. J. Food Microbiol. 2005, 104, 61-67.
163. Pel, H.J.W.; de Winde, J.H.; Archer, D.B.; Dyer, P.S.; Hofmann, G.; Schaap, P.J.; Turner, G.; de Vries, R.P.; Albang, R.; Albermann, K.; et al. Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nat. Biotechnol. 2007, 25, 221-231.
164. Hendrickson, L.; Davis, C.R.; Roach, C.; Nguyen, D.K.; Aldrich, T.; Mcada, P.C.; Reeves, C.D. Lovastatin biosynthesis in Aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene. J. Chem. Biol. 1999, 6, 429-439.
165. Kennedy, J.; Auclair, K.; Kendrew, S.G.; Park, C.; Vederas, J.C.; Hutchinson, C.R. Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis. Science 1999, 284, 1368-1372.
166. Abe, Y.; Suzuki, T.; Ono, C.; Iwamoto, K.; Hosobuchi, M.; Yoshikawa, H. Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum. Mol. Genet. Genom. 2002, 267, 636-646.
167. Kim, Y.T.; Lee, Y.R.; Jin, J.; Han, K.H.; Kim, H.; Kim, J.C.; Lee, T.; Yun, S.H.; Lee, Y.W. Two different polyketide synthase genes are required for synthesis of zearalenone in Gibberella zea. Mol. Microbiol. 2005, 58, 1102-1113.
168. Baker, S.E.; Kroken, S.; Asvarak, T.; Inderbitzin, P.; Li, B.Y.; Shi, L.; Yoder, O.C.; Turgeon, B.G. Two polyketide synthase-encoding genes are required for biosynthesis of the polyketide virulence factor, T-toxin, by Cochliobolus heterostrophus. Mol. Plant Microbe Interact. 2006, 19, 139-149.
169. Botton, A.; Ferrigo, D.; Scopel, C.; Causin, R.; Bonghi, C.; Ramina, A. A cDNA-AFLP approach to study ochratoxin A production in Aspergillus carbonarius. Int. J. Food Microbiol. 2008, 127, 105-115
170. Farber, P.; Geisen, R. Analysis of differentially expressed ochratoxin A biosynthesis genes of Penicillium nordicum. Eur. J. Plant Pathol. 2004, 110, 661-669.
171. Karolewiez, A.; Geisen R. Cloning a part of the OTA biosynthetic gene cluster of Penicillium nordicum and characterization of the ochratoxin polyketide synthase gene. Syst. Appl. Microbiol. 2005, 28, 588-595.
172. Farber, P.; Geisen, R.; Holzapfel, W.H. Detection of aflatoxigenic fungi in figs by a PCR reaction. Int. J. Food Microbiol. 1997, 36, 215-220.
173. Saiki, R.K.; Gelfand, D.H.; Stoffel, S.; Sharf, S.J.; Higuchi, R.; Horn, G.T.; Mullis, K.B.; Ehrlich, H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988, 239, 487-491.
174. Pelegrinelli-Fungaro M.H.; Vissoto P.C., Sartori, D., Vioas-Boas, L.A.; Furlaneto, M.C.; Taniwaki, M.H. A molecular method for detection of Aspergillus carbonarius in coffee beans. Curr. Microbiol. 2004, 49,123-127.
175. Schmidt H.; Ehrmann, M.; Vogel, R.F.; Taniwaki, M.H.; Niessen, L. Molecular typing of Aspergillus ochraceus and construction of species specific SCAR-primers based on AFLP. Syst. Appl. Microbiol. 2003, 26, 434-438.
176. Schmidt, H.; Bannier, M.; Vogel, R.F.; Niessen L. Detection and quantification of Aspergillus ochraceus in green coffee by PCR. Lett. Appl. Microbiol. 2004, 38, 464-469.
177. Schmidt, H.; Taniwaki, M.H.; Vogel, R.F.; Niessen, L. Utilization of AFLP markers for PCRbased identification of Aspergillus carbonarius and indication of its presence in green coffee samples. J. Appl. Microbiol. 2004, 97, 899-909.
178. Vos, P.; Hogers, R.; Bleeker, M.; Reijans, M.; Van de Lee, T.; Hornes, M.; Frijters, A.; Pot, J.; Peleman, J.; Kuiper, M.; Zabeau, M. AFLP: A new technique for DNA fingerprinting. Nucl. Acids Res. 1995, 23, 4407-4414.
179. Castella, G.; Larsen, T.O.; Cabanes, F.J.; Schmidt, H.; Alboresi, A.; Niessen, L.; Farber, P.; Geisen, R. Molecular characterization of ochratoxin A producing strains of the genus Penicillium. Syst. Appl. Microbiol. 2002, 25, 74-83.
180. Perrone, G.; Susca, A.; Stea, G.; Mule, G. PCR assay for identification of Aspergillus carbonarius and Aspergillus japonicus. Eur. J. Plant Pathol. 2004, 110, 641-649.
181. Susca, A.; Stea, G.; Mule, G.; Perrone, G. Polymerase chain reaction (PCR) identification of Aspergillus niger and Aspergillus carbonarius based on the calmodulin gene. Food Addit. Contam. 2007, 24, 1154-1160
182. Mulè, G.; Susca, A.; Logrieco, A.; Stea, G.; Visconti, A. Development of a quantitative real-time PCR assay for the detection of Aspergillus carbonarius in grapes. Int. J. Food Microbiol. 2006, 111, 28-34.
183. Morello, L.G.; Sartori, D.; Martinez, A.L.O.; Carneiro Vieira, M.L.; Taniwaki, M.H.; Pelegrinelli Fungaro, M.H. Detection and quantification of Aspergillus westerdijkiae in coffee beans based on selective amplification of β-tubulin gene by using real-time PCR. Int. J. Food Microbiol. 2007, 119, 270-276
184. White, T.J.; Bruns, T.; Lee, S.; Taylor, J. Amplification and direct sequencing of fungi ribosomal RNA genes for phylogenetics. In PCR Protocols. A Guide to Methods and Applications; Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J., Eds.; Academic Press: San Diego, CA, USA, 1990; pp. 315-322.
185. Martínez-Culebras, P.V.; Ramón, D. An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine. Int. J. Food Microbiol. 2007, 113, 147-153.
186. Kappe, R.; Fauser, C.; Okeke, C.N.; Maiwald, M. Universal fungus-specific primer systems and group-specific hybridization oligonucleotides for 18 rDNA. Mycoses 1996, 39, 25-30.
187. Zhou, G.; Whong, W.Z.; Ong, T.; Chen, B. Development of a fungus-specific PCR assay for detecting low-level fungi in an indoor environment. Mol. Cell Probes 2000, 14, 339-348.
188. Vanittanakom, N.; Vanittanakom, P.; Hay, R.J. Rapid identification of Penicillium marneffei by PCR-based detection of specific sequences on the rRNA gene. J. Clin. Microbiol. 2002, 40, 1739-1742.
189. Hinukson, H.P.; Hurst, S.F.; Lott, T.J.; Warnock, D.N.; Morrison, C.J. Assessment of ribosomal large subunit D1-D2, ITS 1 and ITS 2 regions as targets for molecular identification of medically important Aspergillus species. J. Clin. Microbiol. 2005, 43, 2092-2103.
190. Gonzalez-Salgado, A.; Patno, B.; Vazquez, C.; Gonzalez-Jaen, M.T. Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays. FEMS Microbiol. Lett. 2005, 245, 353-361
191. Patino, B.; Gonzalez-Salgado, A.; Gonzalez-Jaen, M.T.; Vazquez, C. PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species. Int. J Food Microbiol. 2005, 104, 207-214.
192. Accensi, F.; Cano, J.; Figuera, L.; Abarca, M.L.; Cabanes, F.J. New PCR method to differentiate species in the Aspergillus niger aggregate. FEMS Microbiol. Lett. 1999, 180, 191-196.
193. Zanzotto, A.; Burruano, S.; Marciano, P. Digestion of DNA regions to discriminate ochratoxigenic and non-ochratoxigenic strains in the Aspergillus niger aggregate. Int. J. Food Microbiol. 2006, 110, 155-159
194. Niessen, L. Current trends in molecular diagnosis of ochratoxin A producing fungi. In Mycotechnology-Present Status and Future Prospects; Rai, M.; Ed.; I.K. International Publishing House: New Delhi, India, 2007; p. 320.
195. Shapira, R.; Paster, N.; Eyal, O.; Menasherov, M.; Mett, A.; Salomon, R. Detection of aflatoxingenic molds in grains by PCR. Appl. Environ. Microbiol. 1996, 62, 3270-3273.
196. Geisen, R. Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi. Syst. Appl. Microbiol. 1996, 19, 388-392.
197. Niessen, M.L.; Vogel, R.F. Specific identification of Fusarium graminearum by PCR with gaoA targeted primers. Syst. Appl. Microbiol. 1997, 20, 111-123.
198. Paterson, R.R.M. Identification and quantification of mycotoxigenic fungi by PCR. Process Biochem. 2006, 41, 1467-1474
199. Bluhm, B.H.; Flaherty, J.E.; Cousin, M.A.; Woloshuk, C.P. Multiplex polymerase chain reaction assay for the differential detection of trichothecene- and fumonisin-producing species of Fusarium in cornmeal. J. Food Prot. 2002, 65, 1955-1961.
200. Gonzalez-Jaen, M.T.; Mirete, S.; Patino, B.; Lopez-Errasquin, E.; Covadonga, V. Genetic markers for the analysis of variability and for production of specific diagnostic sequences in fumonisin-producing strains of Fusarium verticillioides. Eur. J. Plant Pathol. 2004, 110, 525-532.
201. Paterson, R.R.M. Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples. Food Control 2006, 17, 741-744.
202. Selma, M.V.; Martínez-Culebras, P.V.; Aznar, R. Real-time PCR based procedures for detection and quantification of Aspergillus carbonarius in wine grapes. Int. J. Food Microbiol. 2008, 122, 126-134
203. Atoui, A.; Mathieu, F.; Lebrihi, A. Targeting a polyketide synthase gene for Aspergillus carbonarius quantification and ochratoxin A assessment in grapes using real-time PCR. Int. J. Food Microbiol. 2007, 115, 313-318
204. Witter, C.T.; Herrmann, M.G.; Moss, A.A.; Rasmussen, A.P. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997, 22, 130-138.
205. González-Salgado, A.; Patiño, B.; Gil-Serna, J.; Vázquez, C.; González-Jaén, M.T. Specific detection of Aspergillus carbonarius by SYBR® Green and TaqMan® quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene. FEMS Microbiol. Lett. 2009, 295, 57-66.
206. Livak, K.J.; Flood, S.P.A.; Marmejo, J.; Giusti, W.; Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR products and nucleic acid hybridization. PCR Met. Appl. 1995, 4, 357-362.