Affinity-based, biophysical methods to detect and analyze ligand binding to recombinant proteins: Matching high information content with high throughput

Journal of Structural Biology

Volumn 172, Issue 1, 2010, Pages 142-157

  Holdgate, Geoff A ^a   Anderson, Malcolm ^a   Edfeldt, Fredrik ^b   Geschwindner, Stefan ^b  

^a ASTRAZENECA   (United Kingdom)

^b ASTRAZENECA R AND D   (Sweden)

Author keywords

Affinity; Fragment; Interaction; Ligand; Screening; Thermodynamic

Indexed keywords

BETA SECRETASE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING ASSAY; CENTRIFUGATION; DEUTERIUM HYDROGEN EXCHANGE; DIRECT BINDING ASSAY; ELECTROSPRAY MASS SPECTROMETRY; ENZYMATIC ASSAY; EQUILIBRIUM DIALYSIS; GRATING; INHIBITION IN SOLUTION ASSAY; ION MOBILITY SPECTROMETRY; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OPTICAL BIOSENSOR; OPTICAL WAVEGUIDE GRATING; PRIORITY JOURNAL; SURFACE COMPETITION ASSAY; SURFACE PLASMON RESONANCE; ULTRAFILTRATION;

EID: 77956185674     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2010.06.024     Document Type: Article

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