Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery

Analytical Biochemistry

Volumn 332, Issue 1, 2004, Pages 153-159

  Lo, Mei Chu ^a   Aulabaugh, Ann ^a   Jin, Guixian ^a   Cowling, Rebecca ^a   Bard, Jonathan ^b   Malamas, Michael ^b   Ellestad, George ^a  

^a WYETH RESEARCH   (United States)

^b PFIZER INC   (United States)

Author keywords

BACE1; ITC; Ligand binding affinity; Thermal shift assay

Indexed keywords

BINDING ENERGY; FLUORESCENCE; PROTEINS;

BACE1; BINDING AFFINITIES; ENVIRONMENTALLY SENSITIVE; FLUORESCENCE DETECTION; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING AFFINITY; THERMAL SHIFT; UNFOLDING TEMPERATURE;

LIGANDS;

ENZYME;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CALORIMETRY; COMMERCIAL PHENOMENA; COST; DEVICE; DRUG IDENTIFICATION; ENZYME ASSAY; EVALUATION; FLUORESCENCE; IC 50; ISOTHERM; PRIORITY JOURNAL; TEMPERATURE; TITRIMETRY;

EID: 4143121255     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.04.031     Document Type: Article

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