SPR-based interaction studies with small molecular weight ligands using hAGT fusion proteins

Analytical Biochemistry

Volumn 333, Issue 2, 2004, Pages 280-288

  Huber, Walter ^a   Perspicace, Samantha ^a   Kohler, Josiane ^a   Müller, Francis ^a   Schlatter, Daniel ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Affinity; Binding; Fusion protein; Site directed immobilization

Indexed keywords

AMIDES; BINDING SITES; LIGANDS; RATE CONSTANTS; SURFACE PLASMON RESONANCE;

AFFINITY; BINDING; DIRECTED IMMOBILIZATION; DNA REPAIR PROTEINS; FLUORESCENCE TITRATIONS; FUSION PROTEINS; KINETIC RATE CONSTANTS; SURFACE PLASMON RESONANCE SENSOR;

PROTEINS;

AMINE; CELL EXTRACT; COUMARIN; CYCLOPHILIN; CYCLOSPORIN A; DIAZEPAM; HYBRID PROTEIN; LIGAND; NAPROXEN; O 6 GUANINE DNA TRANSFERASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; TRUNCATED VARIANT OF CYCLOPHILIN D O 6 GUANINE DNA TRANSFERASE; UNCLASSIFIED DRUG; WARFARIN;

ARTICLE; BINDING AFFINITY; CHEMICAL BINDING; COVALENT BOND; DNA BINDING; MOLECULAR INTERACTION; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN TARGETING; SURFACE PLASMON RESONANCE;

EID: 4644340688     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.05.058     Document Type: Article

References (17)

1. Nagata K., Handa H. Real-Time Analysis of Biomolecular Interactions. 2000;Applications of Biacore, Springer-Verlag, Tokyo
2. Buckle M. Surface plasmon resonance applied to DNA-protein complexes. Methods Mol. Biol. 148:2001;535-546
3. McDonnell J.M. Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition. Curr. Opin. Chem. Biol. 5:2001;572-577
4. Satpaev D.K., Slepak V.Z. Analysis of protein-protein interactions in phototransduction cascade using surface plasmon resonance. Methods Enzymol. 316:2000;20-40
5. Adamczyk M., Moore J.A., Yu Z. Application of surface plasmon resonance toward studies of low-molecular-weight antigen-antibody binding interactions. Methods. 20:2000;319-328
6. Hämäläinen M.D., Markgren P.-O., Schaal W., Karlen A., Classon B., Vrang L., Samuelsson B., Hallberg A., Danielson U.H. Characterization of a set of HIV-1 protease inhibitors using binding kinetics data from a biosensor-based screen. J. Biomol. Screen. 5:2000;353-359
7. Myszka D.G., Rich R.L. Implementing surface plasmon resonance biosensors in drug discovery. Pharm. Sci. Technol. Today. 3:2000;310-317
8. Rich R.L., Myszka D.G. Survey of the 2001 commercial optical biosensor literature. J. Mol. Recognit. 15:2002;352-376
9. Myszka D.G. Improving biosensor analysis. J. Mol. Recognit. 12:1999;279-284
10. Van Regenmortel M.H.V. Improving the quality of BIACORE - based affinity measurements. Dev. Biol. 112:2003;141-151
11. Rich R.L., Day Y.S.N., Yasmina S.N., Morton T.A., Myszka D.G. High-resolution and high-throughput protocols for measuring drug/human serum albumin interactions using BIACORE. Anal. Biochem. 296:2001;197-207
12. Kindermann M., George N., Johnsson N., Johnsson K. Covalent and selective immobilization of fusion proteins. J. Am. Chem. Soc. 125:2003;7810-7811
13. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
14. 6-alkylguanine-DNA- alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem. Biol. 10:2003;313-317
15. Davis T.M., Wilson W.D. Determination of the refractive index increments of small molecules for correction of surface plasmon resonance data. Anal. Biochem. 284:2000;348-353
16. Eftink M. Fluorescence methods for studying equilibrium macromolecule-ligand interactions. Methods Enzymol. 278:1997;221-257
17. Johnsson B., Löfås S., Lindquist G. Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. Anal. Biochem. 198:1991;268-277