Free-solution, label-free molecular interactions studied by back-scattering interferometry

Science

Volumn 317, Issue 5845, 2007, Pages 1732-1736

  Bornhop, Darryl J ^a   Latham, Joey C ^a   Kussrow, Amanda ^a   Markov, Dmitry A ^b   Jones, Richard D ^a   Sørensen, Henrik S ^c  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY   (United States)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BLOCKING AGENT; CALCINEURIN; CALCIUM ION; CALMODULIN; IMMUNOGLOBULIN G; INTERLEUKIN 2; M13 PEPTIDE; MONOCLONAL ANTIBODY; PEPTIDE; PROTEIN; UNCLASSIFIED DRUG;

HELIUM; INTERFEROMETRY; MOLECULAR ANALYSIS; SCATTERING;

ANALYTIC METHOD; ARTICLE; BINDING KINETICS; BIOSENSOR; CONCENTRATION RESPONSE; DISSOCIATION CONSTANT; ENERGY TRANSFER; ENTHALPY; EQUILIBRIUM CONSTANT; EQUIPMENT DESIGN; HELIUM NEON LASER; HIGH THROUGHPUT SCREENING; INTERFEROMETRY; ISOTHERMAL TITRATION CALORIMETRY; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; MOLECULAR INTERACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SOLUTION AND SOLUBILITY; SURFACE PROPERTY;

AMINO ACID SEQUENCE; ANIMALS; CALCINEURIN; CALCIUM; CALMODULIN; DIMETHYLPOLYSILOXANES; HUMANS; IMMUNOGLOBULIN G; INTERFEROMETRY; KINETICS; MOLECULAR SEQUENCE DATA; MYOSIN-LIGHT-CHAIN KINASE; PEPTIDE FRAGMENTS; PROTEIN BINDING; RABBITS; REFRACTOMETRY; SILICONES; SOLUTIONS;

EID: 34648815160     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1146559     Document Type: Article

References (53)

1. A. Velazquez-Campoy, E. Freire, Biophys. Chem. 115, 115 (2005).
2. A. Ababou, J. E. Ladbury, J. Mol. Recognit. 20, 4 (2007).
3. S. Leavitt, E. Freire, Curr. Opin. Struct. Biol. 11, 560 (2001).
4. F. E. Torres et al., Proc. Natl. Acad. Sci. U.S.A. 101, 9517 (2004).
5. J. Hahm, C. M. Lieber, Nano Lett. 4, 51 (2004).
6. G. Shekhawat, S. H. Tark, V. P. Dravid, Science 311, 1592 (2006).
7. M. B. Kerby, R. S. Legge, A. Tripathi, Anal. Chem. 78, 8273 (2006).
8. R. L. Rich, Y. S. N. Day, T. A. Morton, D. G. Myszka, Anal. Biochem. 296, 197 (2001).
9. D. A. Markov, K. Swinney, D. J. Bornhop, J. Am. Chem. Soc. 126, 16659 (2004).
10. J. C. Latham, D. A. Markov, H. S. Sorensen, D. J. Bornhop, Angew. Chem. Int. Ed. 45, 955 (2006).
11. F. Brosinger et al., Sens. Actuators B Chem. 44, 350 (1997).
12. F. Prieto et al., Nanotechnology 14, 907 (2003).
13. F. Prieto et al., Sens. Actuators B Chem. 92, 151 (2003).
14. E. F. Schipper et al., Sens. Actuators B Chem. 40, 147 (1997).
15. A. Ymeti et al., Appl. Opt. 42, 5649 (2003).
16. M. J. Swann, L. L. Peel, S. Carrington, N. J. Freeman, Anal. Biochem. 329, 190 (2004).
17. Y. Y. Li et al., Science 299, 2045 (2003).
18. S. L. Pan, L. J. Rothberg, Nano Lett. 3, 811 (2003).
19. J. B. Goh, R. W. Loo, M. C. Goh, Sens. Actuators B Chem. 106, 243 (2005).
20. M. Zhao et al., Clin. Chem. 52, 2135 (2006).
21. J. J. La Clair, M. D. Burkart, Org. Biomol. Chem. 1, 3244 (2003).
22. H. S. Sorensen, N. B. Larsen, J. C. Latham, D. J. Bornhop, P. E. Andersen, Appl. Phys. Lett. 89, 151108 (2006).
23. K. Swinney, D. Markov, D. J. Bornhop, Anal. Chem. 72, 2690 (2000).
24. D. A. Markov, S. Dotson, S. Wood, D. J. Bornhop, Electrophoresis 25, 3805 (2004).
25. D. C. Duffy, J. C. McDonald, O. J. A. Schueller, G. M. Whitesides, Anal. Chem. 70, 4974 (1998).
26. G. J. Wegner, N. J. Lee, G. Marriott, R. M. Corn, Anal. Chem. 75, 4740 (2003).
27. T. Sakai et al., Anal. Sci. 20, 279 (2004).
28. H. Sota, Y. Hasegawa, M. Iwakura, Anal. Chem. 70, 2019 (1998).
29. H. Elkashef, Opt. Mater. 8, 175 (1997).
30. G. Maroulis, D. Xenides, U. Hohm, A. Loose, J. Chem. Phys. 115, 7957 (2001).
31. K. Swinney, D. Markov, D. J. Bornhop, Rev. Sci. Instrum. 71, 2684 (2000).
32. H. J. Tarigan, P. Neill, C. K. Kenmore, D. J. Bornhop, Anal. Chem. 68, 1762 (1996).
33. H. S. Sorensen, Thesis, Technical University of Denmark (2006).
34. B. J. Burke, F. E. Regnier, Anal. Chem. 75, 1786 (2003).
35. G. M. Whitesides, E. Ostuni, S. Takayama, X. Y. Jiang, D. E. Ingber, Annu. Rev. Biomed. Eng. 3, 335 (2001).
36. 2 plasma for 30 s and then placed on a 1-mm-thick microscope glass slide, creating an irreversible bond between the glass and PDMS. The glass slide was used to seal the microfluidic channels and allowed for the entire chip assembly to be handled and securely mounted onto a thermoelectrically temperaturecontrolled x-y translation stage. A shorter top piece of glass was also used as a faceplate, offering structural stability to minimize channel deformations during the sample introduction step. Nanoliter volumes of samples of each of the two binding pairs were placed in the reservoirs at the top of the "Y," then a slight negative pressure was applied to the chip exit well, drawing the two interacting species through the mixer and into the detection zone.
37. In our stop-flow experiments, the sample introduction pressure was chosen to optimize the flow rate and mixing of the solutes. The constraints of the mixer system made it necessary to balance the need for rapid, complete mixing to produce a homogeneous solution of the binding pair with the requirement that the reaction has not proceeded appreciably before flow is stopped and analysis begins. These parameters change slightly for each binding pair, with flow rates found to be in the range of 75 to 120 μL/min.
38. Materials and methods are available as supporting material on Science online.
39. E. Project, R. Friedman, E. Nachliel, M. Gutman, Biophys. J. 90, 3842 (2006).
40. J. J. Langone, Adv. Immunol. 32, 157 (1982).
41. K. Saha, F. Bender, E. Gizeli, Anal. Chem. 75, 835 (2003).
42. C. C. Lee et al., Science 310, 1793 (2005).
43. H. Tsuruta, T. Sano, Biophys. Chem. 35, 75 (1990).
44. L. Massom, H. Lee, H. W. Jarrett, Biochemistry 29, 671 (1990).
45. C. B. Klee, M. H. Krinks, Biochemistry 17, 120 (1978).
46. M. G. Speaker, S. J. Orlow, T. W. Sturgill, O. M. Rosen, Proc. Natl. Acad.Sci. U.S.A. 80, 329 (1983).
47. M. J. Hubbard, C. B. Klee, J. Biol. Chem. 262, 15062 (1987).
48. S. Montigiani, G. Neri, P. Neri, D. Neri, J. Mol. Biol. 258, 6 (1996).
49. K. Török, Biochem. Soc. Trans. 30, 55 (2002).
50. J. Theze, P. M. Alzari, J. Bertoglio, Immunol. Today 17, 481 (1996).
51. A. K. Abbas, A. H. Lichtman, Cellular and Molecular Immunology (Saunders, Philadelphia, ed. 5, 2003).
52. G. H. Reem, N. H. Yeh, D. L. Urdal, P. L. Kilian, J. J. Farrar, Proc. Natl. Acad. Sci. U.S.A. 82, 8663 (1985).
53. This research was supported by National Institute of Biomedical Imaging and Bioengineering at NIH, R-01 EB0003537-01A2, Vanderbilt Institute for Chemical Biology, Air Force Office of Scientific Research grants FA9550-04-1-0364 and FA9550-05-1-0349, and the Vanderbilt Institute for Integrative Biosystems and Education, as well as by vision sciences training grant T32-EY07135 and BIOP Graduate School grant 643-01-0092. We thank J. B. Haas for editorial assistance and P. E. Andersen, S. Dotson, S. Faley, R. Flowers, H. Mchaourab, and J. Wikswo for helpful discussions.