NMR-based screening of proteins containing 13C-labeled methyl groups

Journal of the American Chemical Society

Volumn 122, Issue 33, 2000, Pages 7898-7904

  Hajduk, Philip J ^a   Augeri, David J ^a   Mack, Jamey ^a   Mendoza, Renaldo ^a   Yang, Jianguo ^a   Betz, Stephen F ^a   Fesik, Stephen W ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BUTYRIC ACID DERIVATIVE; CARBON 13; DEUTERIUM; FK 506 BINDING PROTEIN; FRUCTOSE BISPHOSPHATE ALDOLASE; ISOLEUCINE; ISOVALERIC ACID; LEUCINE; MALTOSE BINDING PROTEIN; METHYL GROUP; PROTEIN BCL X; VALINE;

ANALYTIC METHOD; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL LABELING; CONFORMATIONAL TRANSITION; MOLECULAR WEIGHT; PROTEIN ANALYSIS; PROTEIN TARGETING; PROTON NUCLEAR MAGNETIC RESONANCE;

EID: 0034706029     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja000350l     Document Type: Article

References (17)

1. Hajduk, P. J.; Sheppard, G.; Nettesheim, D. G.; Olejniczak, E. T.; Shuker, S. B.; Meadows, R. P.; Steinman, D. H.; Carrera, G. M.; Marcotte, P. A.; Severin, J.; Walter, K.; Smith, H.; Gubbins, E.; Simmer, R.; Holzman, T. F.; Morgan, D. W.; Davidsen, S. K.; Fesik, S. W. J. Am. Chem. Soc. 1997, 119, 5818-5827.
2. Hajduk, P. J.; Dinges, J.; Miknis, G. R.; Merlock, M.; Middleton, T.; Kempf, D. J.; Egan, D. A.; Walter, K. A.; Robins, T. S.; Shuker, S. B.; Holzman, T. F.; Fesik, S. W. J. Med. Chem. 1997, 40, 3144-3150.
3. Hajduk, P. J.; Dinges, J.; Schkeryantz, J. M.; Janowick, D.; Kaminski, M.; Tufano, M.; Augeri, D. J.; Petros, A.; Nienaber, V.; Zhong, P.; Hammond, R.; Coen, M.; Beutel, B.; Katz, L.; Fesik, S. W. J. Med. Chem. 1999, 42, 3852-3859.
4. Hajduk, P. J.; Gerfin, T.; Boehlen, J.-M.; Häberli, M.; Marek, D.; Fesik, S. W. J. Med. Chem. 1999, 42, 2315-2317.
5. Gardner, K. H.; Kay, L. E. J. Am. Chem. Soc. 1997, 119, 7599-7600.
6. Gardner, K. H.; Zhang, X. C.; Gehring, K.; Kay, L. E. J. Am. Chem. Soc.. 1998, 120, 11738-11748.
7. Goto, N. K.; Gardner, K. H.; Mueller, G. A.; Willis, R. C.; Kay, L. E. J. Biomol. NMR 1999, 13, 369-374.
8. Gardner, K. H.; Kay, L. E. Annu. Rev. Biophys. Biomol. Struct. 1998, 27, 357-406.
9. Pervushin, K.; Riek, R.; Wider, G.; Wüthrich, K. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 12336-12371.
10. 3l (Aldrich) and the reported synthetic yields for the reaction shown in Scheme 1) were used.
11. Medek, A.; Hajduk, P. J.; Mack, J.; Fesik, S. W. J. Am. Chem. Soc. 1999, 122, 1241-1242.
12. Sattler, M.; Liang, H.; Nettesheim, D.; Meadows, R. P.; Harlan, J. E.; Eberstadt, M.; Yoon, H. S.; Shuker, S. B.; Chang, B. S.; Minn, A. J.; Thompson, C. B.; Fesik, S. W. Science (Washington, D.C.) 1997, 275, 983-986.
13. Hennig, M.; D'Arcy, A.; Hampele, I. C.; Page, M. G. P.; Oefner, C.; Dale, G. E. Nat. Struct. Biol. 1998, 5, 357-362.
14. Stonehouse, J.; Shaw, G. L.; Keeler, J.; Laue, E. D. J. Magn. Reson. Ser. A 1994, 107, 178-184.
15. Rance, M.; Loria, J. P.; Palmer, A. G. J. Magn. Reson. 1999, 136, 92-101.
16. 15N ratio ∼3.0) in all cases.
17. Fukushima, E.; Roeder, S. B. Experimental Pulse NMR: A Nuts and Bolts Approach; Addison-Wesley Publishing Company, Inc.: Reading, MA, 1981.