-
1
-
-
0028918401
-
A proficient enzyme
-
Radzicka A, Wolfenden R. A proficient enzyme. Science 1995; 267: 90-93.
-
(1995)
Science
, vol.267
, pp. 90-93
-
-
Radzicka, A1
Wolfenden, R.2
-
2
-
-
0037436563
-
Dispelling the myths-biocatalysis in industrial synthesis
-
Schoemaker HE, Mink D, Wubbolts MG. Dispelling the myths-biocatalysis in industrial synthesis. Science 2003; 299: 1694-1697.
-
(2003)
Science
, vol.299
, pp. 1694-1697
-
-
Schoemaker, HE1
Mink, D2
Wubbolts, MG.3
-
3
-
-
0036900263
-
The production of fine chemicals by biotransformations
-
Straathof AJ, Panke S, Schmid A. The production of fine chemicals by biotransformations. Curr Opin Biotech 2002; 13: 548-556.
-
(2002)
Curr Opin Biotech
, vol.13
, pp. 548-556
-
-
Straathof, AJ1
Panke, S2
Schmid, A.3
-
4
-
-
18044398220
-
Directed evolution of biocatalytic processes
-
Hibbert EG, Baganz F, Hailes HC, et al. Directed evolution of biocatalytic processes. Biomol Eng 2005; 22: 11-19.
-
(2005)
Biomol Eng
, vol.22
, pp. 11-19
-
-
Hibbert, EG1
Baganz, F2
Hailes, HC3
-
6
-
-
0036842594
-
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase
-
Glieder A, Farinas ET, Arnold FH. Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nat Biotechnol 2002; 20: 1135-1139.
-
(2002)
Nat Biotechnol
, vol.20
, pp. 1135-1139
-
-
Glieder, A1
Farinas, ET2
Arnold, FH.3
-
7
-
-
33646178621
-
Designed divergent evolution of enzyme function
-
Yoshikuni Y, Ferrin TE, Keasling JD. Designed divergent evolution of enzyme function. Nature 2006; 440: 1078-1082.
-
(2006)
Nature
, vol.440
, pp. 1078-1082
-
-
Yoshikuni, Y1
Ferrin, TE2
Keasling, JD.3
-
8
-
-
0032491867
-
Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution
-
Reetz MT, Zonta A, Schimossek K, Liebeton K, Jaeger KE. Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution. Angew Chem Int Edit 1997; 36: 2830-2832.
-
(1997)
Angew Chem Int Edit
, vol.36
, pp. 2830-2832
-
-
Reetz, MT1
Zonta, A2
Schimossek, K3
Liebeton, K4
Jaeger, KE.5
-
9
-
-
0034059496
-
Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine
-
May O, Nguyen PT, Arnold FH. Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine. Nat Biotechnol 2000; 18: 317-320.
-
(2000)
Nat Biotechnol
, vol.18
, pp. 317-320
-
-
May, O1
Nguyen, PT2
Arnold, FH.3
-
10
-
-
0142214752
-
Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity
-
Carr R, Alexeeva M, Enright A, Eve TSC, Dawson MJ, Turner NJ. Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity. Angew Chem Int Edit 2003; 42: 4807-4810.
-
(2003)
Angew Chem Int Edit
, vol.42
, pp. 4807-4810
-
-
Carr, R1
Alexeeva, M2
Enright, A3
Eve, TSC4
Dawson, MJ5
Turner, NJ.6
-
11
-
-
0034620512
-
Improving the catalytic activity of a thermophilic enzyme at low temperatures
-
Merz A, Yee MC, Szadkowski H, et al. Improving the catalytic activity of a thermophilic enzyme at low temperatures. Biochemistry 2000; 39: 880-889.
-
(2000)
Biochemistry
, vol.39
, pp. 880-889
-
-
Merz, A1
Yee, MC2
Szadkowski, H3
-
12
-
-
0034615775
-
Directed evolution study of temperature adaptation in a psychrophilic enzyme
-
Miyazaki K, Wintrode PL, Grayling RA, Rubingh DN, Arnold FH. Directed evolution study of temperature adaptation in a psychrophilic enzyme. J Mol Biol 2000; 297: 1015-1026.
-
(2000)
J Mol Biol
, vol.297
, pp. 1015-1026
-
-
Miyazaki, K1
Wintrode, PL2
Grayling, RA3
Rubingh, DN4
Arnold, FH.5
-
13
-
-
0034079648
-
Directed evolution of microbial oxidative enzymes
-
Cherry JR. Directed evolution of microbial oxidative enzymes. Current Opin Biotech 2000; 11: 250-254.
-
(2000)
Current Opin Biotech
, vol.11
, pp. 250-254
-
-
Cherry, JR.1
-
14
-
-
0029670577
-
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents
-
Moore JC, Arnold FH. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat Biotechnol 1996; 14: 458-467.
-
(1996)
Nat Biotechnol
, vol.14
, pp. 458-467
-
-
Moore, JC1
Arnold, FH.2
-
15
-
-
18044366809
-
A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents
-
Hao JJ, Berry A. A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents. Protein Eng Des Sel 2004; 17: 689-697.
-
(2004)
Protein Eng Des Sel
, vol.17
, pp. 689-697
-
-
Hao, JJ1
Berry, A.2
-
16
-
-
0033578095
-
Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation
-
Joo H, Lin Z, Arnold FH. Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation. Nature 1999; 399: 670-673.
-
(1999)
Nature
, vol.399
, pp. 670-673
-
-
Joo, H1
Lin, Z2
Arnold, FH.3
-
17
-
-
0042430535
-
Optimising enzyme function by directed evolution
-
Dalby PA. Optimising enzyme function by directed evolution. Curr Opin Struct Biol 2003; 13: 500-505.
-
(2003)
Curr Opin Struct Biol
, vol.13
, pp. 500-505
-
-
Dalby, PA.1
-
18
-
-
0142248496
-
Directed evolution of enzymes for applied biocatalysis
-
Turner NJ. Directed evolution of enzymes for applied biocatalysis. Trends Biotech 2003; 21: 474-478.
-
(2003)
Trends Biotech
, vol.21
, pp. 474-478
-
-
Turner, NJ.1
-
19
-
-
23144436398
-
The FoldX web server: an online force field
-
Schymkowitz J, Borg J, Stricher F, Nys R, Rousseau F, Serrano L. The FoldX web server: an online force field. Nucleic Acids Res 2005; 33: W382-W388.
-
(2005)
Nucleic Acids Res
, vol.33
, pp. W382-W388
-
-
Schymkowitz, J1
Borg, J2
Stricher, F3
Nys, R4
Rousseau, F5
Serrano, L.6
-
20
-
-
33748105087
-
Improving the stability of an antibody variable fragment by a combination of knowledge-based approaches: validation and mechanisms
-
Monsellier E, Bedouelle H. Improving the stability of an antibody variable fragment by a combination of knowledge-based approaches: validation and mechanisms. J Mol Biol 2006; 362: 580-593.
-
(2006)
J Mol Biol
, vol.362
, pp. 580-593
-
-
Monsellier, E1
Bedouelle, H.2
-
21
-
-
33746592898
-
Knowledge-based potentials in protein design
-
Poole AM, Ranganathan R. Knowledge-based potentials in protein design. Curr Opin Struct Biol 2006; 16: 508-513.
-
(2006)
Curr Opin Struct Biol
, vol.16
, pp. 508-513
-
-
Poole, AM1
Ranganathan, R.2
-
22
-
-
0027205210
-
Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
-
Chen K, Arnold FH. Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide. Proc Natl Acad Sci USA 1993; 90: 5618-5622.
-
(1993)
Proc Natl Acad Sci USA
, vol.90
, pp. 5618-5622
-
-
Chen, K1
Arnold, FH.2
-
23
-
-
0028050350
-
Rapid evolution of a protein in vitro by DNA shuffling
-
Stemmer WPC. Rapid evolution of a protein in vitro by DNA shuffling. Nature 1994; 370: 389-391.
-
(1994)
Nature
, vol.370
, pp. 389-391
-
-
Stemmer, WPC.1
-
24
-
-
0031909113
-
Molecular evolution by staggered extension process (StEP) in vitro recombination
-
Zhao H, Giver L, Shao Z, Affholter JA, Arnold FH. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat Biotechnol 1998; 16: 258-261.
-
(1998)
Nat Biotechnol
, vol.16
, pp. 258-261
-
-
Zhao, H1
Giver, L2
Shao, Z3
Affholter, JA4
Arnold, FH.5
-
25
-
-
0035067250
-
DNA shuffling method for generating highly recombined genes and evolved enzymes
-
Coco WM, Levinson WE, Crist MJ, et al. DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat Biotechnol 2001; 19: 354-359.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 354-359
-
-
Coco, WM1
Levinson, WE2
Crist, MJ3
-
26
-
-
17444363724
-
A combinatorial approach to hybrid enzymes independent of DNA homology
-
Ostermeier M, Shim JH, Benkovic SJ. A combinatorial approach to hybrid enzymes independent of DNA homology. Nat Biotechnol 1999; 17: 1205-1209.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 1205-1209
-
-
Ostermeier, M1
Shim, JH2
Benkovic, SJ.3
-
27
-
-
23044501802
-
Evolving strategies for enzyme engineering
-
Bloom JD, Meyer MM, Meinhold P, Otey CR, MacMillan D, Arnold FH. Evolving strategies for enzyme engineering. Curr Opin Struct Biol 2005; 15: 447-452.
-
(2005)
Curr Opin Struct Biol
, vol.15
, pp. 447-452
-
-
Bloom, JD1
Meyer, MM2
Meinhold, P3
Otey, CR4
MacMillan, D5
Arnold, FH.6
-
28
-
-
0032030138
-
Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli
-
Hale RS, Thompson G. Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli. Protein Expres Purif 1998; 12: 185-188.
-
(1998)
Protein Expres Purif
, vol.12
, pp. 185-188
-
-
Hale, RS1
Thompson, G.2
-
29
-
-
0037168524
-
Stabilizing the integrin alpha M inserted domain in alternative conformations with a range of engineered disulfide bonds
-
Shimaoka M, Lu CF, Salas A, Xiao T, Takagi J, Springer TA. Stabilizing the integrin alpha M inserted domain in alternative conformations with a range of engineered disulfide bonds. Proc Natl Acad Sci USA 2002; 99: 16737-16741.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 16737-16741
-
-
Shimaoka, M1
Lu, CF2
Salas, A3
Xiao, T4
Takagi, J5
Springer, TA.6
-
30
-
-
0027155577
-
Engineered Disulfide Bonds As Probes of the Folding Pathway of Barnase-Increasing the Stability of Proteins Against the Rate of Denaturation
-
Clarke J, Fersht AR. Engineered Disulfide Bonds As Probes of the Folding Pathway of Barnase-Increasing the Stability of Proteins Against the Rate of Denaturation. Biochemistry 1993; 32: 4322-4329.
-
(1993)
Biochemistry
, vol.32
, pp. 4322-4329
-
-
Clarke, J1
Fersht, AR.2
-
31
-
-
22844453376
-
The proline rule-A strategy for protein thermal stabilization
-
Suzuki Y. The proline rule-A strategy for protein thermal stabilization. P Jpn Acad B-Phys 1999; 75: 133-137.
-
(1999)
P Jpn Acad B-Phys
, vol.75
, pp. 133-137
-
-
Suzuki, Y.1
-
32
-
-
33745698181
-
Generation and analysis of proline mutants in protein G
-
Choi EJ, Mayo SL. Generation and analysis of proline mutants in protein G. Protein Eng Des Sel 2006; 19: 285-289.
-
(2006)
Protein Eng Des Sel
, vol.19
, pp. 285-289
-
-
Choi, EJ1
Mayo, SL.2
-
33
-
-
0035424955
-
Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution
-
Lehmann M, Wyss M. Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution. Current Opin Biotech 2001; 12: 371-375.
-
(2001)
Current Opin Biotech
, vol.12
, pp. 371-375
-
-
Lehmann, M1
Wyss, M.2
-
34
-
-
23944434781
-
A modified consensus approach to mutagenesis inverts the cofactor specificity of Bacillus stearothermophilus lactate dehydrogenase
-
Flores H, Ellington AD. A modified consensus approach to mutagenesis inverts the cofactor specificity of Bacillus stearothermophilus lactate dehydrogenase. Protein Eng Des Sel 2005; 18: 369-377.
-
(2005)
Protein Eng Des Sel
, vol.18
, pp. 369-377
-
-
Flores, H1
Ellington, AD.2
-
35
-
-
85187239031
-
-
Slovic, A.M., Summa, C.M., Saven, J.G., DeGrado, W.F.: US2004215400 (2004).
-
(2004)
US2004215400
-
-
Slovic, A.M.1
Summa, C.M.2
Saven, J.G.3
DeGrado, W.F.4
-
36
-
-
0037305504
-
Computational design of a water-soluble analog of phospholamban
-
Slovic AM, Summa CM, Lear JD, DeGrado WF. Computational design of a water-soluble analog of phospholamban. Protein Sci 2003; 12: 337-348.
-
(2003)
Protein Sci
, vol.12
, pp. 337-348
-
-
Slovic, AM1
Summa, CM2
Lear, JD3
DeGrado, WF.4
-
37
-
-
17144362921
-
X-ray structure of a water-soluble analog of the membrane protein phospholamban: Sequence determinants defining the topology of tetrameric and pentameric coiled coils
-
Slovic AM, Stayrook SE, North B, DeGrado WF. X-ray structure of a water-soluble analog of the membrane protein phospholamban: Sequence determinants defining the topology of tetrameric and pentameric coiled coils. J Mol Biol 2005; 348: 777-787.
-
(2005)
J Mol Biol
, vol.348
, pp. 777-787
-
-
Slovic, AM1
Stayrook, SE2
North, B3
DeGrado, WF.4
-
38
-
-
0024515763
-
Protein design, a minimalist approach
-
DeGrado WF, Wasserman ZR, Lear JD. Protein design, a minimalist approach. Science 1989; 243: 622-628.
-
(1989)
Science
, vol.243
, pp. 622-628
-
-
DeGrado, WF1
Wasserman, ZR2
Lear, JD.3
-
39
-
-
0025040232
-
Denovo Design, Expression, and Characterization of Felix-A 4-Helix Bundle Protein of Native-Like Sequence
-
Hecht MH, Richardson JS, Richardson DC, Ogden RC. Denovo Design, Expression, and Characterization of Felix-A 4-Helix Bundle Protein of Native-Like Sequence. Science 1990; 249: 884-891.
-
(1990)
Science
, vol.249
, pp. 884-891
-
-
Hecht, MH1
Richardson, JS2
Richardson, DC3
Ogden, RC.4
-
40
-
-
23444436172
-
Protein Design by Binary Patterning of Polar and Nonpolar Amino-Acids
-
Kamtekar S, Schiffer JM, Xiong HY, Babik JM, Hecht MH. Protein Design by Binary Patterning of Polar and Nonpolar Amino-Acids. Science 1993; 262: 1680-1685.
-
(1993)
Science
, vol.262
, pp. 1680-1685
-
-
Kamtekar, S1
Schiffer, JM2
Xiong, HY3
Babik, JM4
Hecht, MH.5
-
41
-
-
0028587717
-
Betadoublet-De-Novo Design, Synthesis, and Characterization of A Beta-Sandwich Protein
-
Quinn TP, Tweedy NB, Williams RW, Richardson JS, Richardson DC. Betadoublet-De-Novo Design, Synthesis, and Characterization of A Beta-Sandwich Protein. Proc Natl Acad Sci USA 1994; 91: 8747-8751.
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 8747-8751
-
-
Quinn, TP1
Tweedy, NB2
Williams, RW3
Richardson, JS4
Richardson, DC.5
-
42
-
-
0345306764
-
Design of a novel globular protein fold with atomic-level accuracy
-
Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D. Design of a novel globular protein fold with atomic-level accuracy. Science 2003; 302: 1364-1368.
-
(2003)
Science
, vol.302
, pp. 1364-1368
-
-
Kuhlman, B1
Dantas, G2
Ireton, GC3
Varani, G4
Stoddard, BL5
Baker, D.6
-
43
-
-
2642670311
-
Design of a 20-amino acid, three-stranded beta-sheet protein
-
Kortemme T, Ramirez-Alvarado M, Serrano L. Design of a 20-amino acid, three-stranded beta-sheet protein. Science 1998; 281: 253-256.
-
(1998)
Science
, vol.281
, pp. 253-256
-
-
Kortemme, T1
Ramirez-Alvarado, M2
Serrano, L.3
-
44
-
-
0036385840
-
Computational de novo design, and characterization of an A(2)B(2) diiron protein
-
Summa CM, Rosenblatt MM, Hong JK, Lear JD, DeGrado WF. Computational de novo design, and characterization of an A(2)B(2) diiron protein. J Mol Biol 2002; 321: 923-938.
-
(2002)
J Mol Biol
, vol.321
, pp. 923-938
-
-
Summa, CM1
Rosenblatt, MM2
Hong, JK3
Lear, JD4
DeGrado, WF.5
-
46
-
-
0038752617
-
Computational design of receptor and sensor proteins with novel functions
-
Looger LL, Dwyer MA, Smith JJ, Hellinga HW. Computational design of receptor and sensor proteins with novel functions. Nature 2003; 423: 185-190.
-
(2003)
Nature
, vol.423
, pp. 185-190
-
-
Looger, LL1
Dwyer, MA2
Smith, JJ3
Hellinga, HW.4
-
47
-
-
33745278855
-
Computational redesign of endonuclease DNA binding and cleavage specificity
-
Ashworth J, Havranek JJ, Duarte CM, et al. Computational redesign of endonuclease DNA binding and cleavage specificity. Nature 2006; 441: 656-659.
-
(2006)
Nature
, vol.441
, pp. 656-659
-
-
Ashworth, J1
Havranek, JJ2
Duarte, CM3
-
48
-
-
0032516785
-
From synthetic coiled coils to functional proteins: Automated design of a receptor for the calmodulin-binding of calcineurin
-
Ghirlanda G, Lear JD, Lombardi A, DeGrado WF. From synthetic coiled coils to functional proteins: Automated design of a receptor for the calmodulin-binding of calcineurin. J Mol Biol 1998; 281: 379-391.
-
(1998)
J Mol Biol
, vol.281
, pp. 379-391
-
-
Ghirlanda, G1
Lear, JD2
Lombardi, A3
DeGrado, WF.4
-
49
-
-
0035807809
-
Enzyme-like proteins by computational design
-
Bolon DN, Mayo SL. Enzyme-like proteins by computational design. P Natl Acad Sci USA 2001; 98: 14274-14279.
-
(2001)
P Natl Acad Sci USA
, vol.98
, pp. 14274-14279
-
-
Bolon, DN1
Mayo, SL.2
-
50
-
-
3042655537
-
Computational design of a biologically active enzyme
-
Dwyer MA, Looger LL, Hellinga HW. Computational design of a biologically active enzyme. Science 2004; 304: 1967-1971.
-
(2004)
Science
, vol.304
, pp. 1967-1971
-
-
Dwyer, MA1
Looger, LL2
Hellinga, HW.3
-
51
-
-
0035059933
-
Proton and metal ion-dependent assembly of a model diiron protein
-
Pasternak A, Kaplan S, Lear JD, DeGrado WF. Proton and metal ion-dependent assembly of a model diiron protein. Protein Sci 2001; 10: 958-969.
-
(2001)
Protein Sci
, vol.10
, pp. 958-969
-
-
Pasternak, A1
Kaplan, S2
Lear, JD3
DeGrado, WF.4
-
52
-
-
0034283867
-
Enzyme catalysis: over-the-barrier or through-the-barrier?
-
Sutcliffe MJ, Scrutton NS. Enzyme catalysis: over-the-barrier or through-the-barrier? Trends Biochem Sci 2000; 25: 405-408.
-
(2000)
Trends Biochem Sci
, vol.25
, pp. 405-408
-
-
Sutcliffe, MJ1
Scrutton, NS.2
-
53
-
-
0036305396
-
A new conceptual framework for enzyme catalysis. Hydrogen tunnelling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes
-
Sutcliffe MJ, Scrutton NS. A new conceptual framework for enzyme catalysis. Hydrogen tunnelling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes. Eur J Biochem 2002; 269: 3096-3102.
-
(2002)
Eur J Biochem
, vol.269
, pp. 3096-3102
-
-
Sutcliffe, MJ1
Scrutton, NS.2
-
54
-
-
33644559358
-
Enzymes: An integrated view of structure, dynamics and function
-
Agarwal PK. Enzymes: An integrated view of structure, dynamics and function. Microb Cell Fact 2006; 5: 2.
-
(2006)
Microb Cell Fact
, vol.5
, pp. 2
-
-
Agarwal, PK.1
-
55
-
-
18844410540
-
Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity
-
Lassila JK, Keeffe JR, Oelschlaeger P, Mayo SL. Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity. Protein Eng Des Sel 2005; 18: 161-163.
-
(2005)
Protein Eng Des Sel
, vol.18
, pp. 161-163
-
-
Lassila, JK1
Keeffe, JR2
Oelschlaeger, P3
Mayo, SL.4
-
56
-
-
0032738825
-
A microfabricated fluorescence-activated cell sorter
-
Fu AY, Spence C, Scherer A, Arnold FH, Quake SR. A microfabricated fluorescence-activated cell sorter. Nat Biotechnol 1999; 17: 1109-1111.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 1109-1111
-
-
Fu, AY1
Spence, C2
Scherer, A3
Arnold, FH4
Quake, SR.5
-
57
-
-
13844250741
-
High-throughput screens and selections of enzyme-encoding genes
-
Aharoni A, Griffiths AD, Tawfik DS. High-throughput screens and selections of enzyme-encoding genes. Curr Opin Chem Biol 2005; 9: 210-216.
-
(2005)
Curr Opin Chem Biol
, vol.9
, pp. 210-216
-
-
Aharoni, A1
Griffiths, AD2
Tawfik, DS.3
-
58
-
-
28844487473
-
High-throughput screening of enzyme libraries: In vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions
-
Mastrobattista E, Taly V, Chanudet E, Treacy P, Kelly BT, Griffiths AD. High-throughput screening of enzyme libraries: In vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions. Chem Biol 2005; 12: 1291-1300.
-
(2005)
Chem Biol
, vol.12
, pp. 1291-1300
-
-
Mastrobattista, E1
Taly, V2
Chanudet, E3
Treacy, P4
Kelly, BT5
Griffiths, AD.6
-
59
-
-
0033772183
-
Function-based isolation of novel enzymes from a large library
-
Olsen MJ, Stephens D, Griffiths D, Daugherty P, Georgiou G, Iverson BL. Function-based isolation of novel enzymes from a large library. Nat Biotechnol 2000; 18: 1071-1074.
-
(2000)
Nat Biotechnol
, vol.18
, pp. 1071-1074
-
-
Olsen, MJ1
Stephens, D2
Griffiths, D3
Daugherty, P4
Georgiou, G5
Iverson, BL.6
-
60
-
-
85187241059
-
-
[60] US2006078888
-
*[60] Griffiths, A.D., Weitz, D., Link, D., Ahn, K., Bibette, J.: US2006078888 (2006).
-
(2006)
-
-
Griffiths, A.D.1
Weitz, D.2
Link, D.3
Ahn, K.4
Bibette, J.5
-
61
-
-
85187214193
-
-
WO06051552
-
Tawfik, D.S., Bernath, K., Magdassi, S., Peisajovich, S.G.: WO06051552 (2006).
-
(2006)
-
-
Tawfik, D.S.1
Bernath, K.2
Magdassi, S.3
Peisajovich, S.G.4
-
62
-
-
28844466075
-
High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments
-
Aharoni A, Amitai G, Bernath K, Magdassi S, Tawfik DS. High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chem Biol 2005; 12: 1281-1289.
-
(2005)
Chem Biol
, vol.12
, pp. 1281-1289
-
-
Aharoni, A1
Amitai, G2
Bernath, K3
Magdassi, S4
Tawfik, DS.5
-
63
-
-
0025988826
-
Random mutagenesis of gene-sized DNA-molecules by use of PCR with Taq DNA-polymerase
-
Zhou YH, Zhang XP, Ebright RH. Random mutagenesis of gene-sized DNA-molecules by use of PCR with Taq DNA-polymerase. Nucleic Acids Res 1991; 19: 6052-6052.
-
(1991)
Nucleic Acids Res
, vol.19
, pp. 6052-6052
-
-
Zhou, YH1
Zhang, XP2
Ebright, RH.3
-
64
-
-
0031909113
-
Molecular evolution by staggered extension process (StEP) in vitro recombination [see comments]
-
Zhao H, Giver L, Shao Z, Affholter JA, Arnold FH. Molecular evolution by staggered extension process (StEP) in vitro recombination [see comments]. Nat Biotechnol 1998; 16: 258-261.
-
(1998)
Nat Biotechnol
, vol.16
, pp. 258-261
-
-
Zhao, H1
Giver, L2
Shao, Z3
Affholter, JA4
Arnold, FH.5
-
67
-
-
33645781503
-
Directed evolution strategies for improved enzymatic performance
-
Hibbert EG, Dalby PA. Directed evolution strategies for improved enzymatic performance. Microb Cell Fact 2005; 4: 29.
-
(2005)
Microb Cell Fact
, vol.4
, pp. 29
-
-
Hibbert, EG1
Dalby, PA.2
-
68
-
-
0031113943
-
An efficient random mutagenesis technique using an Escherichia coli mutator strain
-
Greener A, Callahan M, Jerpseth B. An efficient random mutagenesis technique using an Escherichia coli mutator strain. Mol Biotechnol 1997; 7: 189-195.
-
(1997)
Mol Biotechnol
, vol.7
, pp. 189-195
-
-
Greener, A1
Callahan, M2
Jerpseth, B.3
-
69
-
-
0043194096
-
Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
-
[69]
-
*[69] Camps M, Naukkarinen J, Johnson BP, Loeb LA. Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. P Natl Acad Sci USA 2003; 100: 9727-9732.
-
(2003)
P Natl Acad Sci USA
, vol.100
, pp. 9727-9732
-
-
Camps, M1
Naukkarinen, J2
Johnson, BP3
Loeb, LA.4
-
71
-
-
20544449855
-
Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins
-
Drummond DA, Iverson BL, Georgiou G, Arnold FH. Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins. J Mol Biol 2005; 350: 806-816.
-
(2005)
J Mol Biol
, vol.350
, pp. 806-816
-
-
Drummond, DA1
Iverson, BL2
Georgiou, G3
Arnold, FH.4
-
72
-
-
0035025314
-
Libraries of hybrid proteins from distantly related sequences
-
Sieber V, Martinez CA, Arnold FH. Libraries of hybrid proteins from distantly related sequences. Nat Biotechnol 2001; 19: 456-460.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 456-460
-
-
Sieber, V1
Martinez, CA2
Arnold, FH.3
-
73
-
-
0035949702
-
Creating multiple-crossover DNA libraries independent of sequence identity
-
Lutz S, Ostermeier M, Moore GL, Maranas CD, Benkovic SJ. Creating multiple-crossover DNA libraries independent of sequence identity. P Natl Acad Sci USA 2001; 98: 11248-11253.
-
(2001)
P Natl Acad Sci USA
, vol.98
, pp. 11248-11253
-
-
Lutz, S1
Ostermeier, M2
Moore, GL3
Maranas, CD4
Benkovic, SJ.5
-
74
-
-
0036788537
-
Nucleic acid evolution and minimization by nonhomologous random recombination
-
Bittker JA, Le BV, Liu DR. Nucleic acid evolution and minimization by nonhomologous random recombination. Nat Biotechnol 2002; 20: 1024-1029.
-
(2002)
Nat Biotechnol
, vol.20
, pp. 1024-1029
-
-
Bittker, JA1
Le, BV2
Liu, DR.3
-
76
-
-
0035014185
-
Directed evolution of proteins by exon shuffling
-
Kolkman JA, Stemmer WP. Directed evolution of proteins by exon shuffling. Nat Biotechnol 2001; 19: 423-428.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 423-428
-
-
Kolkman, JA1
Stemmer, WP.2
-
77
-
-
0036960601
-
Structure-based combinatorial protein engineering (SCOPE)
-
O'Maille PE, Bakhtina M, Tsai MD. Structure-based combinatorial protein engineering (SCOPE). J Mol Biol 2002; 321: 677-691.
-
(2002)
J Mol Biol
, vol.321
, pp. 677-691
-
-
O'Maille, PE1
Bakhtina, M2
Tsai, MD.3
-
79
-
-
0033280672
-
Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function
-
Miyazaki K, Arnold FH. Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function. J Mol Evol 1999; 49: 716-720.
-
(1999)
J Mol Evol
, vol.49
, pp. 716-720
-
-
Miyazaki, K1
Arnold, FH.2
-
83
-
-
85187232560
-
-
Bauer, J.C., Wright, D.A., Braman, J.C., Geha, R.S.: US5932419 (1999).
-
(1999)
US5932419
-
-
Bauer, J.C.1
Wright, D.A.2
Braman, J.C.3
Geha, R.S.4
-
85
-
-
85187219261
-
-
Padgett, H.S., Vaewhongs, A.A., Vojdani, F.S., Smith, M.L., Lindbo, J.A., Fitzmaurice, W.P.: WO03066809 (2003).
-
(2003)
WO03066809
-
-
Padgett, H.S.1
Vaewhongs, A.A.2
Vojdani, F.S.3
Smith, M.L.4
Lindbo, J.A.5
Fitzmaurice, W.P.6
-
86
-
-
0035917812
-
Expanding the Genetic Code of Escherichia coli
-
Wang L, Brock A, Herberich B, Schultz PG. Expanding the Genetic Code of Escherichia coli. Science 2001; 292: 498-500.
-
(2001)
Science
, vol.292
, pp. 498-500
-
-
Wang, L1
Brock, A2
Herberich, B3
Schultz, PG.4
-
87
-
-
85187236270
-
-
[87] US2005250183
-
*[87] Schultz, P.G., Wang, L., Andersen, J.C., Chin, J.W., Liu, D.R., Magliery, T.J., Meggers, E.L., Mehl, R.A., Pastrnak, M., Santoro, S.W., Zhang, Z.: US2005250183 (2005).
-
(2005)
-
-
Schultz, P.G.1
Wang, L.2
Andersen, J.C.3
Chin, J.W.4
Liu, D.R.5
Magliery, T.J.6
Meggers, E.L.7
Mehl, R.A.8
Pastrnak, M.9
Santoro, S.W.10
Zhang, Z.11
-
90
-
-
0035957360
-
Computational method to reduce the search space for directed protein evolution
-
Voigt CA, Mayo SL, Arnold FH, Wang ZG. Computational method to reduce the search space for directed protein evolution. Proc Natl Acad Sci USA 2001; 98: 3778-3783.
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 3778-3783
-
-
Voigt, CA1
Mayo, SL2
Arnold, FH3
Wang, ZG.4
-
91
-
-
0035748334
-
Computationally focusing the directed evolution of proteins
-
Suppl 37
-
Voigt CA, Mayo SL, Arnold FH, Wang ZG. Computationally focusing the directed evolution of proteins. J Cell Biochem Suppl 2001; Suppl 37: 58-63.
-
(2001)
J Cell Biochem Suppl
, pp. 58-63
-
-
Voigt, CA1
Mayo, SL2
Arnold, FH3
Wang, ZG.4
-
92
-
-
85187226709
-
-
Voigt, C.A., Wang, Z.G., Arnold, F.H., Mayo, S.L.: WO03091835 (2003).
-
(2003)
WO03091835
-
-
Voigt, C.A.1
Wang, Z.G.2
Arnold, F.H.3
Mayo, S.L.4
-
93
-
-
0037059061
-
Combining computational and experimental screening for rapid optimization of protein properties
-
Hayes RJ, Bentzien J, Ary ML, Hwang MY, Jacinto JM, Vielmetter J, Kundu A, Dahiyat BI. Combining computational and experimental screening for rapid optimization of protein properties. P Natl Acad Sci USA 2002; 99: 15926-15931.
-
(2002)
P Natl Acad Sci USA
, vol.99
, pp. 15926-15931
-
-
Hayes, RJ1
Bentzien, J2
Ary, ML3
Hwang, MY4
Jacinto, JM5
Vielmetter, J6
Kundu, A7
Dahiyat, BI.8
-
94
-
-
85187218212
-
-
[94]
-
*[94] Mayo, S.L., Dahiyat, B.I., Gordon, D.B., Street, A., Su, Y.: US2006019316 (2006).
-
(2006)
US2006019316
-
-
Mayo, S.L.1
Dahiyat, B.I.2
Gordon, D.B.3
Street, A.4
Su, Y.5
-
99
-
-
85187219208
-
-
Tang, X.-S., Cheng, Q., Shyr, J.Y., Tao, L.: WO06072078 (2006).
-
(2006)
WO06072078
-
-
Tang, X.-S.1
Cheng, Q.2
Shyr, J.Y.3
Tao, L.4
-
100
-
-
85187216284
-
-
WO06065076
-
Pan, J.G., Jung, H.C., Kim, E.J., Lee, H.S., Park, Y.H., Kim, H.S., Han, J.K., Lee, J.N., Oh, K.H., Kim, J.H., Oh, Y.S., Sim, J.I., Hong, K.K., Choi, K.O., Kim, H.S., Baek, M.J., Kang, T.S.: WO06065076 (2006).
-
(2006)
-
-
Pan, J.G.1
Jung, H.C.2
Kim, E.J.3
Lee, H.S.4
Park, Y.H.5
Kim, H.S.6
Han, J.K.7
Lee, J.N.8
Oh, K.H.9
Kim, J.H.10
Oh, Y.S.11
Sim, J.I.12
Hong, K.K.13
Choi, K.O.14
Kim, H.S.15
Baek, M.J.16
Kang, T.S.17
-
103
-
-
85187238817
-
-
Ajinomoto, K.K., Mihara, Y., Utagawa, T., Hideaki, Y., Asano, Y.: WO9637603 (1996).
-
(1996)
WO9637603
-
-
Ajinomoto, K.K.1
Mihara, Y.2
Utagawa, T.3
Hideaki, Y.4
Asano, Y.5
-
106
-
-
85187219634
-
-
Svendsen, A., Kjaerulff, S., Bisgaard-Frantzen, H., Andersen, C.: EP1676913 (2006).
-
(2006)
EP1676913
-
-
Svendsen, A.1
Kjaerulff, S.2
Bisgaard-Frantzen, H.3
Andersen, C.4
-
110
-
-
0037163083
-
Biochemical characterization of the chondroitinase B active site
-
Pojasek K, Raman R, Kiley P, Venkataraman G, Sasisekharan R. Biochemical characterization of the chondroitinase B active site. J Biol Chem 2002; 277: 31179-31186.
-
(2002)
J Biol Chem
, vol.277
, pp. 31179-31186
-
-
Pojasek, K1
Raman, R2
Kiley, P3
Venkataraman, G4
Sasisekharan, R.5
|