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84920312598
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H2O have to be taken with caution because of the small change in fluorescence between folded and unfolded states and the large error in the estimation of the slopes of the urea dependence of the fluorescence of the folded and unfolded states, arising from the solvent exposure of the Trp side chain.
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HNα coupling constants. With a set of 40 sequential, 48 nonsequential, and 11 dihedral restraints, a family of 45 structures without NOE violations larger than 0.2 Å were generated using DYANA [P. Güntert, C. Mumenthaler, K. Wüthrich, J. Mol. Biol. 273, 283 (1997)]. The rms deviation from the average minimized structure in the well-ordered region of residues 3-18 was 0.96 Å for the backbone atoms and 1.93 Å for all heavy atoms.
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84920312596
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note
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We thank M. J. Macías and H. Oschkinat for communicating results before publication; H. van der Zandt for help with the sedimentation equilibrium measurements; T. Creighton and M. Saraste for critical reading of the manuscript; M. Sattler for discussions concerning NMR experiments; and M. Petukhov, E. Lacroix, and J. Martínez for helpful discussions. This work was partly supported by a European Union Biotechnology grant (BIO4-CT97-2086).
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