High-throughput screening of enzyme libraries: In vitro evolution of a β-galactosidase by fluorescence-activated sorting of double emulsions

Chemistry and Biology

Volumn 12, Issue 12, 2005, Pages 1291-1300

  Mastrobattista, Enrico ^a,b   Taly, Valerie ^a,c   Chanudet, Estelle ^a   Treacy, Patrick ^a,c   Kelly, Bernard T ^a   Griffiths, Andrew D ^a,c  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UTRECHT UNIVERSITY   (Netherlands)

^c UNIVERSITÉ LOUIS PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; EBGR PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; REPRESSOR PROTEIN;

ARTICLE; CHEMICAL STRUCTURE; DIRECTED MOLECULAR EVOLUTION; EMULSION; ENZYMOLOGY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE LIBRARY; GENETICS; METABOLISM; METHODOLOGY; MUTATION; PROTEIN ENGINEERING; TIME;

BETA-GALACTOSIDASE; DIRECTED MOLECULAR EVOLUTION; EMULSIONS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLOW CYTOMETRY; GENE LIBRARY; MODELS, MOLECULAR; MUTATION; PROTEIN ENGINEERING; REPRESSOR PROTEINS; TIME FACTORS;

ESCHERICHIA COLI;

EID: 28844487473     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2005.09.016     Document Type: Article

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