An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 7, Issue 2, 1997, Pages 189-195

  Greener, Alan ^a   Callahan, Marie ^b   Jerpseth, Bruce ^b  

^a Stratagene   (United States)

^b NONE

Author keywords

E. coli; Mutator; mutD; mutS; mutT; Random mutagenesis; XL1 Red

Indexed keywords

ARTICLE; ESCHERICHIA COLI; GENETICS; MUTAGENESIS;

ESCHERICHIA COLI; MUTAGENESIS;

EID: 0031113943     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02761755     Document Type: Article

References (10)

1. Miller, J. H. (1992) A Short Course in Bacterial Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Greener, A. and Callahan, M. (1994) XL1-RED: a highly efficient random mutagenesis strain. Strategies 7, 32-34.
3. Radman, M., Wagner, R. E., Glickman, B. W., and Meselson, M. (1980) DNA methylation, mismatch correction, and genetic stability, in Progress in Environmental Mutagenesis (Alevic, M., ed.), Elsevier, Amsterdam, pp. 121-130.
4. Cox, E. C. (1976) Bacterial mutator genes and the control of spontaneous mutation. Ann. Rev. Genet. 10, 135-156.
5. Scheueurmann, R., Tam, S., Burgers, P. M. J., Lu, C., and Echols, H. (1983) Identification of the e-subunit of Escherichia coli DNA polymerase III holo-enzyme as the dnaQ gene product: a fidelity subunit for DNA replication. Proc. Natl. Acad. Sci. USA 80, 7085-7089.
6. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
7. Tomizawa, J. and Som, T. (1984) Control of CoIE1 plasmid replication: enhancement of binding of RNA 1 to the primer transcript by the rom protein. Cell 38, 871-878.
8. Lacatena, R. M., Banner, D. W., Castagnoli, L., and Cesareni, G. (1984) Control of initiation of pMB1 replication: purified rop protein and RNA 1 affect primer formation in vitro. Cell 37, 1009-1014.
9. Uhlin, B. E., Molin, S., Gustafsson, P., and Nordstrom, K. (1979) Plasmids with temperature dependent copy numbers for amplification of cloned genes and their products. Gene 6, 91-103.
10. Harley, C. B. and Reynolds, R. P. (1987) Analysis of E. coli promoter sequences. Nucleic Acids Res. 15, 2343-2361.