Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli

Protein Expression and Purification

Volumn 12, Issue 2, 1998, Pages 185-188

  Hale, Richard S ^a   Thompson, Gladstone ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CLONED GENE; CODON; GLUTATHIONE TRANSFERASE; PROTEIN EXPRESSION; TYPE 1 NEUROFIBROMIN;

ESCHERICHIA COLI; TRIXIS;

EID: 0032030138     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0825     Document Type: Article

References (8)

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3. Wang B. Q., Lei L., Burton Z. F. Importance of codon preference for production of human RAP74 and reconstitution of the RAP30/74 complex. Prot. Express. Purif. 5:1994;476-485.
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5. Eccleston J. F., Moore K. J. M., Morgan L., Skinner R. H., Lowe P. N. Kinetics of interaction between normal and proline 12 Ras and the GTPase activating proteins p120-GAP and neurofibromin. J. Biol. Chem. 268:1993;27012-27019.
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7. Stemmer W. P., Crameri A., Ha K. D., Brennan T. M., Heyneker H. L. Single step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene. 164:1995;49-53.
8. Keohavong P., Thilly W. G. Fidelity of DNA polymerases in DNA amplification. Proc. Natl. Acad. Sci. USA. 86:1989;9253-9257.