Proton and metal ion-dependent assembly of a model diiron protein

Protein Science

Volumn 10, Issue 5, 2001, Pages 958-969

  Pasternak, A ^a   Kaplan, J ^a   Lear, J D ^a   DeGrado, W F ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

De novo protein design; Diiron; Four helix bundle; Metalloprotein design

Indexed keywords

IRON DERIVATIVE; METAL ION; PROTEIN DERIVATIVE; PROTON; ZINC DERIVATIVE;

ARTICLE; CRYSTAL STRUCTURE; GEOMETRY; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN SYNTHESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; CATIONS, DIVALENT; DIMERIZATION; ELECTROSTATICS; GUANIDINE; HYDROGEN-ION CONCENTRATION; IRON; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NONHEME IRON PROTEINS; OXIDATION-REDUCTION; OXYGEN; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN SUBUNITS; PROTONS; THERMODYNAMICS; ULTRACENTRIFUGATION;

EID: 0035059933     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.52101     Document Type: Article

References (44)

1. Unusual clustering of carboxyl side chains in the core of iron-free ribonucleotide reductase
2. Diiron-oxygen proteins
3. The crystal structure of an azide complex of the diferrous R2 subunit of ribonucleotide reductase displays a novel carboxylate shift with important mechanistic implications for diiron-catalyzed oxygen activation
4. Construction of a novel redox protein by rational design: Conversion of a disulfide bridge into a mononuclear iron-sulfur center
5. Rational design of nascent metalloenzymes
6. High spin cobalt(II) as a probe for the investigation of metalloproteins
7. Mechanism of assembly of the tyrosyl radical-dinuclear iron cluster cofactor of ribonucleotide reductase
8. Selective recognition and cleavage of RNA loop structures by Ni(II) Xaa-Gly-His metallopeptides
9. Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (Ferrozine)
10. Design of a heme-binding four-helix bundle
11. Use of the polymerase chain reaction for the rapid construction of synthetic genes
12. Reactions of non-heme iron(II) centers with dioxygen in biology and chemistry
13. Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins
14. Structure of a unique twofold symmetric haem-binding site
15. The XL-I analytical ultracentrifuge with Rayleigh interference optics
16. Ferredoxin and ferredoxin-heme maquettes
17. 2+-binding protein
18. Metal ion-dependent modulation of the dynamics of a designed protein
19. Kinetic and thermodynamic studies of the copper (II) and nickel (II) complexes of Glycylglycyl-L-histidine
20. The construction of metal centers in proteins by rational design
21. Spectroscopic studies of cobalt(II) binding to Escherichia coli bacterioferritin
22. Partial volumes and compressibilities of extended polypeptide chains in aqueous solution: Addititivity scheme and implication of protein unfolding at normal and high pressure
23. Oxygen activating nonheme iron enzymes
24. + release associated with cobalt(II) and zinc(II) binding at dinuclear metal sites
25. 9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins
26. Retrostructural analysis of metalloproteins: Application to the design of a minimal model for diiron proteins
27. Di-iron-carboxylate proteins
28. A designed metal-binding protein with a novel fold
29. Construction of a catalytically active iron superoxide dismutase by rational design
30. Toward the synthesis of a photosynthetic reaction center maquette: A cofacial porphyrin pair assembled between two subunits of a synthetic four-helix bundle multiheme protein
31. Protein design: Novel metal-binding sites
32. A tetrahedal zinc(II)-binding site introduced into a designed protein
33. Design and synthesis of multi-haem proteins
34. L-conformation at the ends of helices
35. The Sa1-XH motif for metal-mediated oxidative DNA-peptide cross-linking
36. Models of the reduced forms of polyiron-oxo proteins: An asymmetric, triply carboxylate bridged diiron(II) complex and its reaction with dioxygen
37. Exploiting polypeptide motifs for the design of selective Cu(II) ion chemosensors
38. A molecular modeling and drug design program
39. Dioxygen activation by enzymes containing binuclear non-heme iron clusters
40. Reaction paths of iron oxidation and hydrolysis in horse spleen and recombinant human ferritins
41. The iron oxidation and hydrolysis chemistry of Escherichia coli bacterioferritin