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Volumn 8, Issue 5, 2017, Pages 315-327

Genome engineering of stem cell organoids for disease modeling

Author keywords

genome editing; pluripotent adult stem cell; precision medicine; tissue organoid

Indexed keywords

DISEASE COURSE; DISEASE MODEL; EPIGENETICS; GENE EDITING; GENE EXPRESSION; GENETIC DISORDER; GENETIC ENGINEERING; GENETIC VARIATION; GENOTYPE PHENOTYPE CORRELATION; HUMAN; INDUCED PLURIPOTENT STEM CELL; MEDICAL RESEARCH; NEVUS; NUTRITIONAL STATUS; PERSONALIZED MEDICINE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; REVIEW; SUBMUCOUS PLEXUS; TOXICITY TESTING; ANIMAL; BIOLOGICAL MODEL; CYTOPLASM; METABOLISM; PROCEDURES;

EID: 85009935501     PISSN: 1674800X     EISSN: 16748018     Source Type: Journal    
DOI: 10.1007/s13238-016-0368-0     Document Type: Review
Times cited : (26)

References (106)
  • 2
    • 84988592852 scopus 로고    scopus 로고
    • Inheritable silencing of endogenous genes by hit-and-run targeted epigenetic editing
    • COI: 1:CAS:528:DC%2BC28XhsFKmtbfN, PID: 27662090
    • Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A (2016) Inheritable silencing of endogenous genes by hit-and-run targeted epigenetic editing. Cell 167:219–232
    • (2016) Cell , vol.167 , pp. 219-232
    • Amabile, A.1    Migliara, A.2    Capasso, P.3    Biffi, M.4    Cittaro, D.5    Naldini, L.6    Lombardo, A.7
  • 5
    • 84925858253 scopus 로고    scopus 로고
    • Modeling human nutrition using human embryonic stem cells
    • COI: 1:CAS:528:DC%2BC2MXls1agsrY%3D, PID: 25815980
    • Ben-Zvi D, Melton DA (2015) Modeling human nutrition using human embryonic stem cells. Cell 161:12–17
    • (2015) Cell , vol.161 , pp. 12-17
    • Ben-Zvi, D.1    Melton, D.A.2
  • 13
    • 84923762812 scopus 로고    scopus 로고
    • A new initiative on precision medicine
    • COI: 1:CAS:528:DC%2BC2MXjvFGnsLY%3D, PID: 25635347
    • Collins FS, Varmus H (2015) A new initiative on precision medicine. N Engl J Med 372:793–795
    • (2015) N Engl J Med , vol.372 , pp. 793-795
    • Collins, F.S.1    Varmus, H.2
  • 15
    • 84919623629 scopus 로고    scopus 로고
    • Celluar heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population
    • COI: 1:CAS:528:DC%2BC2cXhslOrtL3E, PID: 25373907
    • DeWard AD, Cramer J, Lagasse E (2014) Celluar heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population. Cell Rep 9:701–711
    • (2014) Cell Rep , vol.9 , pp. 701-711
    • DeWard, A.D.1    Cramer, J.2    Lagasse, E.3
  • 21
    • 84930999403 scopus 로고    scopus 로고
    • Interaction of Salmonella enterica Serovar Typhimurium with intestinal organoids derived from human induced pluripotent stem cells
    • COI: 1:CAS:528:DC%2BC2MXhtFarur7I, PID: 25964470
    • Forbester JL, Goulding D, Vallier L, Hannan N, Hale C, Pickard D, Mukhopadhyay S, Dougan G (2015) Interaction of Salmonella enterica Serovar Typhimurium with intestinal organoids derived from human induced pluripotent stem cells. Infect Immun 83:2926–2934
    • (2015) Infect Immun , vol.83 , pp. 2926-2934
    • Forbester, J.L.1    Goulding, D.2    Vallier, L.3    Hannan, N.4    Hale, C.5    Pickard, D.6    Mukhopadhyay, S.7    Dougan, G.8
  • 27
    • 84889563027 scopus 로고    scopus 로고
    • Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells
    • PID: 24232854
    • Hisha H, Tanaka T, Kanno S, Tokuyama Y, Komai Y, Ohe S, Yanai H, Omachi T, Ueno H (2013) Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells. Sci Rep 3:3224
    • (2013) Sci Rep , vol.3 , pp. 3224
    • Hisha, H.1    Tanaka, T.2    Kanno, S.3    Tokuyama, Y.4    Komai, Y.5    Ohe, S.6    Yanai, H.7    Omachi, T.8    Ueno, H.9
  • 28
    • 84902096048 scopus 로고    scopus 로고
    • Development and applications of CRISPR-Cas9 for genome engineering
    • COI: 1:CAS:528:DC%2BC2cXpslagt7s%3D, PID: 24906146
    • Hsu PD, Lander ES, Zhang F (2014) Development and applications of CRISPR-Cas9 for genome engineering. Cell 157:1262–1278
    • (2014) Cell , vol.157 , pp. 1262-1278
    • Hsu, P.D.1    Lander, E.S.2    Zhang, F.3
  • 35
    • 84928924333 scopus 로고    scopus 로고
    • Functional annotation of native enhancers with a Cas9-histone demethylase fusion
    • COI: 1:CAS:528:DC%2BC2MXkvVynsL8%3D, PID: 25775043
    • Kearns NA, Pham H, Tabak B, Genga RM, Silverstein NJ, Garber M, Maehr R (2015) Functional annotation of native enhancers with a Cas9-histone demethylase fusion. Nat Methods 12:401–403
    • (2015) Nat Methods , vol.12 , pp. 401-403
    • Kearns, N.A.1    Pham, H.2    Tabak, B.3    Genga, R.M.4    Silverstein, N.J.5    Garber, M.6    Maehr, R.7
  • 36
    • 84901849749 scopus 로고    scopus 로고
    • 3D mouse embryonic stem cell culture for generating inner ear organoids
    • COI: 1:CAS:528:DC%2BC2cXnt1emur4%3D, PID: 24784820
    • Koehler KR, Hashino E (2014) 3D mouse embryonic stem cell culture for generating inner ear organoids. Nat Protoc 9:1229–1244
    • (2014) Nat Protoc , vol.9 , pp. 1229-1244
    • Koehler, K.R.1    Hashino, E.2
  • 37
    • 84881474378 scopus 로고    scopus 로고
    • Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture
    • COI: 1:CAS:528:DC%2BC3sXhtFShs77O, PID: 23842490
    • Koehler KR, Mikosz AM, Molosh AI, Patel D, Hashino E (2013) Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture. Nature 500:217–221
    • (2013) Nature , vol.500 , pp. 217-221
    • Koehler, K.R.1    Mikosz, A.M.2    Molosh, A.I.3    Patel, D.4    Hashino, E.5
  • 38
    • 84898665052 scopus 로고    scopus 로고
    • Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
    • COI: 1:CAS:528:DC%2BC3sXhvFylsL%2FF, PID: 24535568
    • Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K (2014) Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol 32:267–273
    • (2014) Nat Biotechnol , vol.32 , pp. 267-273
    • Koike-Yusa, H.1    Li, Y.2    Tan, E.P.3    Velasco-Herrera Mdel, C.4    Yusa, K.5
  • 39
    • 84971006562 scopus 로고    scopus 로고
    • Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
    • COI: 1:CAS:528:DC%2BC28XmsVehsr8%3D, PID: 27096365
    • Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533:420–424
    • (2016) Nature , vol.533 , pp. 420-424
    • Komor, A.C.1    Kim, Y.B.2    Packer, M.S.3    Zuris, J.A.4    Liu, D.R.5
  • 41
    • 84971529257 scopus 로고    scopus 로고
    • Repression of the antioxidant NRF2 pathway in premature aging
    • COI: 1:CAS:528:DC%2BC28Xpslaltrg%3D, PID: 27259148
    • Kubben N, Zhang W, Wang L, Voss TC, Yang J, Qu J, Liu GH, Misteli T (2016) Repression of the antioxidant NRF2 pathway in premature aging. Cell 165:1361–1374
    • (2016) Cell , vol.165 , pp. 1361-1374
    • Kubben, N.1    Zhang, W.2    Wang, L.3    Voss, T.C.4    Yang, J.5    Qu, J.6    Liu, G.H.7    Misteli, T.8
  • 42
    • 84926039213 scopus 로고    scopus 로고
    • Generation of cerebral organoids from human pluripotent stem cells
    • COI: 1:CAS:528:DC%2BC2cXhsVyhu7%2FO, PID: 25188634
    • Lancaster MA, Knoblich JA (2014) Generation of cerebral organoids from human pluripotent stem cells. Nat Protoc 9:2329–2340
    • (2014) Nat Protoc , vol.9 , pp. 2329-2340
    • Lancaster, M.A.1    Knoblich, J.A.2
  • 44
    • 84893436164 scopus 로고    scopus 로고
    • Lung stem cell differentiation in mice directed by endothelial cells via a BMP4-NFATc1-thrombospondin-1 axis
    • COI: 1:CAS:528:DC%2BC2cXhs1Kgs78%3D, PID: 24485453
    • Lee JH, Bhang DH, Beede A, Huang TL, Stripp BR, Bloch KD, Wagers AJ, Tseng YH, Ryeom S, Kim CF (2014) Lung stem cell differentiation in mice directed by endothelial cells via a BMP4-NFATc1-thrombospondin-1 axis. Cell 156:440–455
    • (2014) Cell , vol.156 , pp. 440-455
    • Lee, J.H.1    Bhang, D.H.2    Beede, A.3    Huang, T.L.4    Stripp, B.R.5    Bloch, K.D.6    Wagers, A.J.7    Tseng, Y.H.8    Ryeom, S.9    Kim, C.F.10
  • 45
    • 84919478713 scopus 로고    scopus 로고
    • Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function
    • PID: 25312952
    • Leslie JL, Huang S, Opp JS, Nagy MS, Kobayashi M, Young VB, Spence JR (2015) Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function. Infect Immun 83:138–145
    • (2015) Infect Immun , vol.83 , pp. 138-145
    • Leslie, J.L.1    Huang, S.2    Opp, J.S.3    Nagy, M.S.4    Kobayashi, M.5    Young, V.B.6    Spence, J.R.7
  • 46
    • 84983073946 scopus 로고    scopus 로고
    • Vitamin C alleviates aging defects in a stem cell model for Werner syndrome
    • COI: 1:CAS:528:DC%2BC28XhtVSht7zL, PID: 27271327
    • Li Y, Zhang W, Chang L, Han Y, Sun L, Gong X, Tang H, Liu Z, Deng H, Ye Y (2016) Vitamin C alleviates aging defects in a stem cell model for Werner syndrome. Protein Cell 7:478–488
    • (2016) Protein Cell , vol.7 , pp. 478-488
    • Li, Y.1    Zhang, W.2    Chang, L.3    Han, Y.4    Sun, L.5    Gong, X.6    Tang, H.7    Liu, Z.8    Deng, H.9    Ye, Y.10
  • 48
    • 84966908028 scopus 로고    scopus 로고
    • Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
    • COI: 1:CAS:528:DC%2BC28Xmt1eksbg%3D, PID: 27088723
    • Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T (2016a) Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol 34:528–530
    • (2016) Nat Biotechnol , vol.34 , pp. 528-530
    • Ma, H.1    Tu, L.C.2    Naseri, A.3    Huisman, M.4    Zhang, S.5    Grunwald, D.6    Pederson, T.7
  • 49
    • 84990898361 scopus 로고    scopus 로고
    • Targeted AID-mediated mutagenesis(TAM) enables efficient genomic diversification in mammalian cells
    • PID: 27723754
    • Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X (2016b) Targeted AID-mediated mutagenesis(TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. doi:10.1038/nmeth.4027
    • (2016) Nat Methods
    • Ma, Y.1    Zhang, J.2    Yin, W.3    Zhang, Z.4    Song, Y.5    Chang, X.6
  • 53
    • 84924422753 scopus 로고    scopus 로고
    • Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids
    • COI: 1:CAS:528:DC%2BC2MXjtFemsLg%3D, PID: 25706875
    • Matano M, Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T (2015) Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Nat Med 21:256–262
    • (2015) Nat Med , vol.21 , pp. 256-262
    • Matano, M.1    Date, S.2    Shimokawa, M.3    Takano, A.4    Fujii, M.5    Ohta, Y.6    Watanabe, T.7    Kanai, T.8    Sato, T.9
  • 54
    • 80955137949 scopus 로고    scopus 로고
    • Generating human intestinal tissue from pluripotent stem cells in vitro
    • COI: 1:CAS:528:DC%2BC3MXhsFyltbrM, PID: 22082986
    • McCracken KW, Howell JC, Wells JM, Spence JR (2011) Generating human intestinal tissue from pluripotent stem cells in vitro. Nat Protoc 6:1920–1928
    • (2011) Nat Protoc , vol.6 , pp. 1920-1928
    • McCracken, K.W.1    Howell, J.C.2    Wells, J.M.3    Spence, J.R.4
  • 57
    • 84909957872 scopus 로고    scopus 로고
    • Engineering de novo assembly of fetal pulmonary organoids
    • COI: 1:CAS:528:DC%2BC2cXhvFSntb7N, PID: 24825442
    • Mondrinos MJ, Jones PL, Finck CM, Lelkes PI (2014) Engineering de novo assembly of fetal pulmonary organoids. Tissue Eng Part A 20:2892–2907
    • (2014) Tissue Eng Part A , vol.20 , pp. 2892-2907
    • Mondrinos, M.J.1    Jones, P.L.2    Finck, C.M.3    Lelkes, P.I.4
  • 59
    • 84922791023 scopus 로고    scopus 로고
    • Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells
    • COI: 1:CAS:528:DC%2BC2MXhvVOqtbw%3D, PID: 25640179
    • Muguruma K, Nishiyama A, Kawakami H, Hashimoto K, Sasai Y (2015) Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells. Cell Rep 10:537–550
    • (2015) Cell Rep , vol.10 , pp. 537-550
    • Muguruma, K.1    Nishiyama, A.2    Kawakami, H.3    Hashimoto, K.4    Sasai, Y.5
  • 60
    • 84880026566 scopus 로고    scopus 로고
    • Genome editing of human pluripotent stem cells to generate human cellular disease models
    • COI: 1:CAS:528:DC%2BC3sXht1Chsr3N, PID: 23751357
    • Musunuru K (2013) Genome editing of human pluripotent stem cells to generate human cellular disease models. Dis Model Mech 6:896–904
    • (2013) Dis Model Mech , vol.6 , pp. 896-904
    • Musunuru, K.1
  • 67
    • 84954138949 scopus 로고    scopus 로고
    • Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells
    • COI: 1:CAS:528:DC%2BC28Xos1Kgug%3D%3D, PID: 26762480
    • Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y (2016) Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells. Nat Commun 7:10351
    • (2016) Nat Commun , vol.7 , pp. 10351
    • Ozone, C.1    Suga, H.2    Eiraku, M.3    Kadoshima, T.4    Yonemura, S.5    Takata, N.6    Oiso, Y.7    Tsuji, T.8    Sasai, Y.9
  • 71
    • 84874687019 scopus 로고    scopus 로고
    • Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression
    • COI: 1:CAS:528:DC%2BC3sXjsFChs7s%3D, PID: 23452860
    • Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152:1173–1183
    • (2013) Cell , vol.152 , pp. 1173-1183
    • Qi, L.S.1    Larson, M.H.2    Gilbert, L.A.3    Doudna, J.A.4    Weissman, J.S.5    Arkin, A.P.6    Lim, W.A.7
  • 79
    • 84981714781 scopus 로고    scopus 로고
    • Live cell imaging reveals the dynamics of telomerase recruitment to telomeres
    • COI: 1:CAS:528:DC%2BC28XhtleisrbP, PID: 27523609
    • Schmidt JC, Zaug AJ, Cech TR (2016) Live cell imaging reveals the dynamics of telomerase recruitment to telomeres. Cell 166:1188–1197
    • (2016) Cell , vol.166 , pp. 1188-1197
    • Schmidt, J.C.1    Zaug, A.J.2    Cech, T.R.3
  • 80
    • 84979518893 scopus 로고    scopus 로고
    • Modeling human disease using organotypic cultures
    • COI: 1:CAS:528:DC%2BC28XhtFymsr%2FO, PID: 27474805
    • Schweiger PJ, Jensen KB (2016) Modeling human disease using organotypic cultures. Curr Opin Cell Biol 43:22–29
    • (2016) Curr Opin Cell Biol , vol.43 , pp. 22-29
    • Schweiger, P.J.1    Jensen, K.B.2
  • 82
    • 84942892037 scopus 로고    scopus 로고
    • Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death
    • COI: 1:CAS:528:DC%2BC2MXhsFersL3M, PID: 26375003
    • Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, Zhuang Y, Cai T, Wang F, Shao F (2015) Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526:660–665
    • (2015) Nature , vol.526 , pp. 660-665
    • Shi, J.1    Zhao, Y.2    Wang, K.3    Shi, X.4    Wang, Y.5    Huang, H.6    Zhuang, Y.7    Cai, T.8    Wang, F.9    Shao, F.10
  • 84
    • 84885672388 scopus 로고    scopus 로고
    • Differentiated Troy+ chife cells act as reserve stem cells to generate all lineages of the stomach epithelium
    • COI: 1:CAS:528:DC%2BC3sXhs1SktLnL, PID: 24120136
    • Stange DE, Koo BK, Huch M, Sibbel G, Basak O, Lyubimova A, Kujala P, Bartfeld S, Koster J, Geahlen JH et al (2013) Differentiated Troy+ chife cells act as reserve stem cells to generate all lineages of the stomach epithelium. Cell 155:357–368
    • (2013) Cell , vol.155 , pp. 357-368
    • Stange, D.E.1    Koo, B.K.2    Huch, M.3    Sibbel, G.4    Basak, O.5    Lyubimova, A.6    Kujala, P.7    Bartfeld, S.8    Koster, J.9    Geahlen, J.H.10
  • 86
    • 36248966518 scopus 로고    scopus 로고
    • Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    • COI: 1:CAS:528:DC%2BD2sXhsVCntbbK, PID: 18035408
    • Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
    • (2007) Cell , vol.131 , pp. 861-872
    • Takahashi, K.1    Tanabe, K.2    Ohnuki, M.3    Narita, M.4    Ichisaka, T.5    Tomoda, K.6    Yamanaka, S.7
  • 89
    • 84893223076 scopus 로고    scopus 로고
    • Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant
    • COI: 1:CAS:528:DC%2BC2cXls1KmtLo%3D, PID: 24457331
    • Takebe T, Zhang RR, Koike H, Kimura M, Yoshizawa E, Enomura M, Koike N, Sekine K, Taniguchi H (2014) Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. Nat Protoc 9:396–409
    • (2014) Nat Protoc , vol.9 , pp. 396-409
    • Takebe, T.1    Zhang, R.R.2    Koike, H.3    Kimura, M.4    Yoshizawa, E.5    Enomura, M.6    Koike, N.7    Sekine, K.8    Taniguchi, H.9
  • 93
    • 84994504451 scopus 로고    scopus 로고
    • Applications of CRISPR genome engineering in cell biology
    • COI: 1:CAS:528:DC%2BC28XhsVams7bI, PID: 27599850
    • Wang F, Qi LS (2016) Applications of CRISPR genome engineering in cell biology. Trends Cell Biol 26:875–888
    • (2016) Trends Cell Biol , vol.26 , pp. 875-888
    • Wang, F.1    Qi, L.S.2
  • 94
    • 84892749369 scopus 로고    scopus 로고
    • Genetic screens in human cells using the CRISPR-Cas9 system
    • COI: 1:CAS:528:DC%2BC2cXkslOq, PID: 24336569
    • Wang T, Wei JJ, Sabatini DM, Lander ES (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Science 343:80–84
    • (2014) Science , vol.343 , pp. 80-84
    • Wang, T.1    Wei, J.J.2    Sabatini, D.M.3    Lander, E.S.4
  • 97
    • 84922753974 scopus 로고    scopus 로고
    • A small intestinal organoid model of non-invasive enteric pathogen-epithelial cell interactions
    • COI: 1:CAS:528:DC%2BC2cXhtlCit77O, PID: 25118165
    • Wilson SS, Tocchi A, Holly MK, Parks WC, Smith JG (2015) A small intestinal organoid model of non-invasive enteric pathogen-epithelial cell interactions. Mucosal Immunol 8:352–361
    • (2015) Mucosal Immunol , vol.8 , pp. 352-361
    • Wilson, S.S.1    Tocchi, A.2    Holly, M.K.3    Parks, W.C.4    Smith, J.G.5
  • 100
    • 84894623720 scopus 로고    scopus 로고
    • Niche-independent high-purify cultures of Lgr5+ intestinal stem cells and their progeny
    • COI: 1:CAS:528:DC%2BC3sXhvVGru7nM, PID: 24292484
    • Yin X, Farin HF, van Es JH, Clevers H, Langer R, Karp JM (2014) Niche-independent high-purify cultures of Lgr5+ intestinal stem cells and their progeny. Nat Methods 11:106–112
    • (2014) Nat Methods , vol.11 , pp. 106-112
    • Yin, X.1    Farin, H.F.2    van Es, J.H.3    Clevers, H.4    Langer, R.5    Karp, J.M.6
  • 103
    • 85009424076 scopus 로고    scopus 로고
    • Salmonella-infected crypt-derived intestinal organoid culture system for host-bacterial interactions
    • Zhang YG, Wu S, Xia Y, Sun J (2014) Salmonella-infected crypt-derived intestinal organoid culture system for host-bacterial interactions. Physiol Rep 2(9):e12147
    • (2014) Physiol Rep , vol.2 , Issue.9 , pp. e12147
    • Zhang, Y.G.1    Wu, S.2    Xia, Y.3    Sun, J.4
  • 104
    • 84929435164 scopus 로고    scopus 로고
    • A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging
    • COI: 1:CAS:528:DC%2BC2MXpt1KlsLY%3D, PID: 25931448
    • Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A et al (2015) A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science 348:1160–1163
    • (2015) Science , vol.348 , pp. 1160-1163
    • Zhang, W.1    Li, J.2    Suzuki, K.3    Qu, J.4    Wang, P.5    Zhou, J.6    Liu, X.7    Ren, R.8    Xu, X.9    Ocampo, A.10
  • 105
    • 84900861730 scopus 로고    scopus 로고
    • High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells
    • COI: 1:CAS:528:DC%2BC2cXotlymtrg%3D, PID: 24717434
    • Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W (2014) High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature 509:487–491
    • (2014) Nature , vol.509 , pp. 487-491
    • Zhou, Y.1    Zhu, S.2    Cai, C.3    Yuan, P.4    Li, C.5    Huang, Y.6    Wei, W.7
  • 106
    • 85003712738 scopus 로고    scopus 로고
    • Genome-scale deletion screening of human long non-coding RNAs using a paired-guided RNA CRISPR-Cas9 library
    • Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J et al (2016) Genome-scale deletion screening of human long non-coding RNAs using a paired-guided RNA CRISPR-Cas9 library. Nat Biotechnol. doi: 10.1038/nbt.3715
    • (2016) Nat Biotechnol
    • Zhu, S.1    Li, W.2    Liu, J.3    Chen, C.H.4    Liao, Q.5    Xu, P.6    Xu, H.7    Xiao, T.8    Cao, Z.9    Peng, J.10


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