Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements

Nature Methods

Volumn 12, Issue 12, 2015, Pages 1143-1149

  Thakore, Pratiksha I ^a,b   D'Ippolito, Anthony M ^b   Song, Lingyun ^b,c   Safi, Alexias ^b,c   Shivakumar, Nishkala K ^a   Kabadi, Ami M ^a,b   Reddy, Timothy E ^b,c   Crawford, Gregory E ^b,c   Gersbach, Charles A ^a,b,c  

^a Duke University   (United States)

^b DUKE UNIVERSITY   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUIDE RNA; TRANSCRIPTION FACTOR; CRISPR ASSOCIATED PROTEIN; GLOBIN; REPRESSOR PROTEIN; VIRAL PROTEIN;

ARTICLE; CHROMATIN STRUCTURE; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; EMBRYO; ENHANCER REGION; EPIGENETICS; GENE CONTROL; GENE FUSION; GENE SILENCING; GLOBIN GENE; HETEROCHROMATIN; HISTONE METHYLATION; HUMAN; HUMAN CELL; K562 CELL LINE; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; REPRESSOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; TRANSIENT TRANSFECTION; WESTERN BLOTTING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE EXPRESSION REGULATION; GENETIC EPIGENESIS; GENETICS; HEK293 CELL LINE; K-562 CELL LINE; LENTIVIRINAE; PROCEDURES; REGULATORY SEQUENCE;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; ENHANCER ELEMENTS, GENETIC; EPIGENESIS, GENETIC; EPIGENOMICS; GENE EXPRESSION REGULATION, VIRAL; GLOBINS; HEK293 CELLS; HUMANS; K562 CELLS; LENTIVIRUS; REGULATORY ELEMENTS, TRANSCRIPTIONAL; REPRESSOR PROTEINS; RNA, GUIDE; VIRAL PROTEINS;

EID: 84949100864     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3630     Document Type: Article

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