Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells

Nature Communications

Volumn 7, Issue , 2016, Pages

  Ozone, Chikafumi ^a,b   Suga, Hidetaka ^b   Eiraku, Mototsugu ^a   Kadoshima, Taisuke ^a   Yonemura, Shigenobu ^a,c   Takata, Nozomu ^a   Oiso, Yutaka ^b   Tsuji, Takashi ^a   Sasai, Yoshiki ^a  

^a RIKEN Center for Biosystems Dynamics Research   (Japan)

^b NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HYPOPHYSIS HORMONE; CORTICOTROPIN; GROWTH HORMONE;

CELL ORGANELLE; EMBRYO; ENDOCRINE SYSTEM; HOMEOSTASIS; HORMONE;

ACTH SECRETING CELL; ADENOHYPOPHYSIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ECTODERM; HOST; HUMAN; HUMAN CELL; HUMAN EMBRYONIC STEM CELL; HYPOPHYSIS; HYPOPHYSIS TISSUE; HYPOPITUITARISM; IN VITRO STUDY; MOUSE; NONHUMAN; PHYSICAL ACTIVITY; SURVIVAL; ANIMAL; CELL CULTURE TECHNIQUE; EMBRYO DEVELOPMENT; EMBRYOLOGY; EMBRYONIC STEM CELL; PHYSIOLOGY; PROCEDURES; SECRETION (PROCESS); TISSUE ENGINEERING; TRANSPLANTATION;

MUS;

ADRENOCORTICOTROPIC HORMONE; ANIMALS; CELL CULTURE TECHNIQUES; CORTICOTROPHS; EMBRYONIC DEVELOPMENT; EMBRYONIC STEM CELLS; GROWTH HORMONE; HUMANS; HYPOPITUITARISM; MICE; PITUITARY GLAND, ANTERIOR; TISSUE ENGINEERING;

EID: 84954138949     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms10351     Document Type: Article

References (37)

1. Kelberman, D., Rizzoti, K., Lovell-Badge, R., Robinson, I. C. & Dattani, M. T. Genetic regulation of pituitary gland development in human and mouse. Endocr. Rev. 30, 790-829 (2009).
2. Romero, C. J., Nesi-França, S. & Radovick, S. The molecular basis of hypopituitarism. Trends Endocrinol. Metab. 20, 506-516 (2009).
3. Dincer, Z. et al. Specification of functional cranial placode derivatives from human pluripotent stem cells. Cell Rep. 5, 1387-1402 (2013).
4. Takuma, N. et al. Formation of Rathke's pouch requires dual induction from the diencephalon. Development 125, 4835-4840 (1998).
5. Suh, H., Gage, P. J., Drouin, J. & Camper, S. A. Pitx2 is required at multiple stages of pituitary organogenesis: pituitary primordium formation and cell specification. Development 129, 329-337 (2002).
6. Zhu, X., Gleiberman, A. S. & Rosenfeld, M. G. Molecular physiology of pituitary development: signaling and transcriptional networks. Physiol. Rev. 87, 933-963 (2007).
7. Rizzoti, K. & Lovell-Badge, R. Early development of the pituitary gland: induction and shaping of Rathke's pouch. Rev. Endocr. Metab. Disord. 6, 161-172 (2005).
8. Ericson, J., Norlin, S., Jessell, T. M. & Edlund, T. Integrated FGF and BMP signaling controls the progression of progenitor cell differentiation and the emergence of pattern in the embryonic anterior pituitary. Development 125, 1005-1015 (1998).
9. Vankelecom, H. Pituitary stem/progenitor cells: embryonic players in the adult gland? Eur. J. Neurosci. 32, 2063-2081 (2010).
10. Eiraku, M. et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 472, 51-56 (2011).
11. Suga, H. et al. Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480, 57-62 (2011).
12. Wataya, T. et al. Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation. Proc. Natl Acad. Sci. USA 105, 11796-11801 (2008).
13. Watanabe, K. et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681-686 (2007).
14. Nakano, T. et al. Self-formation of optic cups and storable stratified neural retina from human ES cells. Cell Stem Cell 10, 771-785 (2012).
15. Treier, M. et al. Hedgehog signaling is required for pituitary gland development. Development 128, 377-386 (2001).
16. Wilson, P. A. & Hemmati-Brivanlou, A. Induction of epidermis and inhibition of neural fate by Bmp-4. Nature 376, 331-333 (1995).
17. Watanabe, K. et al. Directed differentiation of telencephalic precursors from embryonic stem cells. Nat. Neurosci. 8, 288-296 (2005).
18. Basch, M. L. & Bronner-Fraser, M. Neural crest inducing signals. Adv. Exp. Med. Biol. 589, 24-31 (2006).
19. Ellsworth, B. S., Butts, D. L. & Camper, S. A. Mechanisms underlying pituitary hypoplasia and failed cell specification in Lhx3-deficient mice. Dev. Biol. 313, 118-129 (2008).
20. Sheng, H. Z. et al. Specification of pituitary cell lineages by the LIM homeobox gene Lhx3. Science 272, 1004-1007 (1996).
21. Bharti, K., Gasper, M., Bertuzzi, S. & Arnheiter, H. Lack of the ventral anterior homeodomain transcription factor VAX1 leads to induction of a second pituitary. Development 138, 873-878 (2011).
22. Zhang, X. et al. Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family. J. Biol. Chem. 281, 15694-15700 (2006).
23. Ornitz, D. M. et al. Receptor specificity of the fibroblast growth factor family. J. Biol. Chem. 271, 15292-15297 (1996).
24. Lanctôt, C., Lamolet, B. & Drouin, J. The bicoid-related homeoprotein Ptx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm. Development 124, 2807-2817 (1997).
25. Lamolet, B. et al. A pituitary cell-restricted T box factor, Tpit, activates POMC transcription in cooperation with Pitx homeoproteins. Cell 104, 849-859 (2001).
26. Hemming, F. J., Bégeot, M., Dubois, M. P. & Dubois, P. M Fetal rat somatotropes in vitro: effects of insulin, cortisol, and growth hormone-releasing factor on their differentiation: a light and electron microscopic study. Endocrinology 114, 2107-2113 (1984).
27. Davis, S. W. et al. Molecular mechanisms of pituitary organogenesis: in search of novel regulatory genes. Mol. Cell. Endocrinol. 323, 4-19 (2010).
28. Pelletier, G., Leclerc, R. & Labrie, F. Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique. Mol. Cell Endocrinol. 6, 123-128 (1976).
29. Ingle, D. J. The effects of administering large amounts of cortin on the adrenal cortices of normal and hypophysectomized rats. Am. J. Physiol. 124, 369-371 (1938).
30. Giustina, A. & Veldhuis, J. D. Pathophysiology of the neuroregulation of growth hormone secretion in experimental animals and the human. Endocr. Rev. 19, 717-797 (1998).
31. Porter, T. E. & Dean, K. J. Regulation of chicken embryonic growth hormone secretion by corticosterone and triiodothyronine: evidence for a negative synergistic response. Endocrine 14, 363-368 (2001).
32. Lin, W. J., Singer, G. & Irby, D. Effect of hypophysectomy on schedule-induced wheelrunning. Pharmacol. Biochem. Behav. 35, 739-742 (1990).
33. Schwartz, S. D. et al. Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1/2 studies. Lancet 385, 509-516 (2015).
34. Reardon, S. & Cyranoski, D. Japan stem-cell trial stirs envy. Nature 513, 287-288 (2014).
35. Watanabe, K. et al. Directed differentiation of telencephalic precursors from embryonic stem cells. Nat. Neurosci. 8, 288-296 (2005).
36. Obata, Y. et al. Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis. J. Immunol. 188, 2427-2436 (2012).
37. Falconi, G. & Rossi, G. L. Transauricular hypophysectomy in rats and mice. Endocrinology 74, 301-303 (1964).