Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium

Gastroenterology

Volumn 141, Issue 5, 2011, Pages 1762-1772

  Sato, Toshiro ^a   Stange, Daniel E ^a   Ferrante, Marc ^a,b   Vries, Robert G J ^a   Van Es, Johan H ^a   Van Den Brink, Stieneke ^a   Van Houdt, Winan J ^a,c   Pronk, Apollo ^c   Van Gorp, Joost ^c   Siersema, Peter D ^a   Clevers, Hans ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b UNIVERSITY HOSPITALS LEUVEN   (Belgium)

^c DEPARTMENT OF SURGERY   (Netherlands)

Author keywords

Dysplasia; Ex Vivo Culture Technology; Hayflick Model; Neoplasia; Signaling

Indexed keywords

NICOTINAMIDE; WNT3A PROTEIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BARRETT ESOPHAGUS; CANCER CELL; CELL EXPANSION; COLON ADENOCARCINOMA; COLON ADENOMA; CONTROLLED STUDY; CYTOPLASM; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 80054857419     PISSN: 00165085     EISSN: 15280012     Source Type: Journal    
DOI: 10.1053/j.gastro.2011.07.050     Document Type: Article

References (41)

1. Evans GS, Flint N, Somers AS, et al. The development of a method for the preparation of rat intestinal epithelial cell primary cultures. J Cell Sci 1992;101:219-231.
2. Fukamachi H. Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture. J Cell Sci 1992;103:511-519.
3. Perreault N, Jean-Francois B. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Exp Cell Res 1996;224:354-364. (Pubitemid 26140569)
4. Whitehead RH, Demmler K, Rockman SP, et al. Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans. Gastroenterology 1999;117:858-865. (Pubitemid 29468228)
5. Ootani A, Li X, Sangiorgi E, et al. Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med 2009;15:701-706.
6. Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 2009;459:262-265.
7. Korinek V, Barker N, Moerer P, et al. Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nat Genet 1998;19:379-383. (Pubitemid 28357912)
8. Pinto D, Gregorieff A, Begthel H, et al. Canonical Wnt signals are essential for homeostasis of the intestinal epithelium. Genes Dev 2003;17:1709-1713. (Pubitemid 36870334)
9. Kuhnert F, Davis CR, Wang HT, et al. Essential requirement for Wnt signaling in proliferation of adult small intestine and colon revealed by adenoviral expression of Dickkopf-1. Proc Natl Acad Sci U S A 2004;101:266-271. (Pubitemid 38084239)
10. Kim KA, Kakitani M, Zhao J, et al. Mitogenic influence of human R-spondin1 on the intestinal epithelium. Science 2005;309:1256-1259. (Pubitemid 41170922)
11. Barker N, van Es JH, Kuipers J, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 2007;449:1003-1007. (Pubitemid 350014593)
12. de Lau W, Barker N, Low TY, et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 2011;476:293-297.
13. Dignass AU, Sturm A. Peptide growth factors in the intestine. Eur J Gastroenterol Hepatol 2001;13:763-770. (Pubitemid 32656588)
14. Haramis AP, Begthel H, van den Born M, et al. De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine. Science 2004;303:1684-1686. (Pubitemid 38338329)
15. Hofmann C, Obermeier F, Artinger M, et al. Cell-cell contacts prevent anoikis in primary human colonic epithelial cells. Gastroenterology 2007;132:587-600. (Pubitemid 46274657)
16. Sasaki T, Giltay R, Talts U, et al. Expression and distribution of laminin alpha1 and alpha2 chains in embryonic and adult mouse tissues: an immunochemical approach. Exp Cell Res 2002;275:185-199. (Pubitemid 34948361)
17. Rogler G, Gelbmann CM, Vogl D, et al. Differential activation of cytokine secretion in primary human colonic fibroblast/myofibroblast cultures. Scand J Gastroenterol 2001;36:389-398. (Pubitemid 32250735)
18. Buset M, Winawer S, Friedman E. Defining conditions to promote the attachment of adult human colonic epithelial cells. In Vitro Cell Dev Biol 1987;23:403-412.
19. Whitehead RH, Brown A, Bhathal PS. A method for the isolation and culture of human colonic crypts in collagen gels. In Vitro Cell Dev Biol 1987;23:436-442.
20. Deveney CW, Rand-Luby L, Rutten MJ, et al. Establishment of human colonic epithelial cells in long-term culture. J Surg Res 1996;64:161-169. (Pubitemid 26304887)
21. Pang G, Buret A, O'Loughlin E, et al. Immunologic, functional, and morphological characterization of three new human small intestinal epithelial cell lines. Gastroenterology 1996;111:8-18. (Pubitemid 26226878)
22. Latella G, Fonti R, Caprilli R, et al. Characterization of the mucins produced by normal human colonocytes in primary culture. Int J Colorectal Dis 1996;11:76-83. (Pubitemid 26119240)
23. Panja A. A novel method for the establishment of a pure population of nontransformed human intestinal primary epithelial cell (HIPEC) lines in long term culture. Lab Invest 2000;80:1473-1475.
24. Grossmann J, Walther K, Artinger M, et al. Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC). Eur J Cell Biol 2003;82:262-270. (Pubitemid 36656572)
25. Sansom OJ, Reed KR, Hayes AJ, et al. Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration. Genes Dev 2004;18:1385-1390. (Pubitemid 38780625)
26. Lustig B, Jerchow B, Sachs M, et al. Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors. Mol Cell Biol 2002;22:1184-1193. (Pubitemid 34094854)
27. Booth C, O'Shea JA. Isolation and culture of intestinal epithelial cells. In: Freshney RI and Freshney MG, ed. Culture of epithelial cells. 2nd ed. New York, NY:Wiley-Liss 2002:303-335.
28. Gregorieff A, Pinto D, Begthel H, et al. Expression pattern of Wnt signaling components in the adult intestine. Gastroenterology 2005;129:626-638. (Pubitemid 41096648)
29. Sato T, van Es JH, Snippert HJ, et al. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 2011;469:415-418.
30. van de Wetering M, Sancho E, Verweij C, et al. The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 2002;111:241-250. (Pubitemid 35292444)
31. Denu JM. Vitamin B3 and sirtuin function. Trends Biochem Sci 2005;30:479-483.
32. Shay JW, Wright WE. Telomerase therapeutics for cancer: challenges and new directions. Nat Rev Drug Discov 2006;5:577-584. (Pubitemid 43971192)
33. Otsuka M, Kang YJ, Ren J, et al. Distinct effects of p38alpha deletion in myeloid lineage and gut epithelia in mouse models of inflammatory bowel disease. Gastroenterology 2010;138:1255-1265, 1265 e1-9.
34. van Es JH, van Gijn ME, Riccio O, et al. Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells. Nature 2005;435:959-963. (Pubitemid 40896322)
35. Barker N, Ridgway RA, van Es JH, et al. Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 2009;457:608-611.
36. Odze RD. Barrett esophagus: histology and pathology for the clinician. Nat Rev Gastroenterol Hepatol 2009;6:478-490.
37. Menke V, van Es JH, de Lau W, et al. Conversion of metaplastic Barrett's epithelium into post-mitotic goblet cells by gamma-secretase inhibition. Dis Model Mech 2010;3:104-110.
38. Dey D, Saxena M, Paranjape AN, et al. Phenotypic and functional characterization of human mammary stem/progenitor cells in long term culture. PloS One 2009;4:e5329.
39. Garraway IP, Sun W, Tran CP, et al. Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo. Prostate 2010;70:491-501.
40. Walen KH. Spontaneous cell transformation: karyoplasts derived from multinucleated cells produce new cell growth in senescent human epithelial cell cultures. In Vitro Cell Dev Biol Anim 2004;40:150-158. (Pubitemid 39383084)
41. Hayflick L. The cell biology of aging. J Invest Dermatol 1979;73:8-14. (Pubitemid 10136102)