Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Protein Expression and Purification

Volumn 92, Issue 1, 2013, Pages 67-76

  Hacker, David L ^a   Kiseljak, Divor ^a   Rajendra, Yashas ^a   Thurnheer, Sarah ^a   Baldi, Lucia ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

CHO DG44; HEK 293; Mammalian cells; Orbital shaking; Polyethylenimine; Recombinant protein; Suspension culture; Transfection; Transient gene expression

Indexed keywords

DRUG CARRIER; POLYETHYLENEIMINE; RECOMBINANT PROTEIN;

ANIMAL; CELL STRAIN HEK293; CHO CELL; CHO-DG44; CRICETULUS; CULTURE TECHNIQUE; EQUIPMENT; EQUIPMENT DESIGN; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HAMSTER; HEK-293; HUMAN; MAMMALIAN CELLS; METABOLISM; METHODOLOGY; ORBITAL SHAKING; REVIEW; SUSPENSION CELL CULTURE; TRANSIENT EXPRESSION; VIRUS;

CHO-DG44; HEK-293; MAMMALIAN CELLS; ORBITAL SHAKING; POLYETHYLENIMINE; RECOMBINANT PROTEIN; SUSPENSION CULTURE; TRANSFECTION; TRANSIENT GENE EXPRESSION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; DRUG CARRIERS; EQUIPMENT DESIGN; GENETIC VECTORS; HEK293 CELLS; HUMANS; POLYETHYLENEIMINE; RECOMBINANT PROTEINS; TRANSFECTION; VIRUSES;

EID: 84884736571     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.09.001     Document Type: Review

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