High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI

Biotechnology and Bioengineering

Volumn 99, Issue 3, 2008, Pages 721-727

  Backliwal, Gaurav ^a   Hildinger, Markus ^b   Hasija, Vivek ^a   Wurm, Florian M ^a,b  

^a EPFL   (Switzerland)

^b EXCELLGENE SA   (Switzerland)

Author keywords

HEK 293; High density; PEI; Recombinant protein; Transient transfection

Indexed keywords

CELLS; DNA SEQUENCES; GENE EXPRESSION; MAMMALS; POLYETHYLENES;

CELL DENSITIES; POLYETHYLENEIMINE (PEI); RECOMBINANT PROTEIN; TRANSFECTION PROTOCOL; TRANSIENT TRANSFECTION;

PROTEINS;

POLYETHYLENEIMINE; RECOMBINANT PROTEIN; DNA; DRUG CARRIER;

ARTICLE; CELL STRAIN HEK293; CELL SUSPENSION; COMPLEX FORMATION; GENE EXPRESSION; GENETIC TRANSFECTION; HIGH DENSITY TRANSFECTION; HUMAN; HUMAN CELL; PRIORI DNA COMPLEX FORMATION; CELL LINE; CHEMISTRY; GENETICS; KIDNEY; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN ENGINEERING;

MAMMALIA;

CELL LINE; DNA; DRUG CARRIERS; HUMANS; KIDNEY; POLYETHYLENEIMINE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 38449121649     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21596     Document Type: Article

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