A simple high-yielding process for transient gene expression in CHO cells

Journal of Biotechnology

Volumn 153, Issue 1-2, 2011, Pages 22-26

  Rajendra, Yashas ^a   Kiseljak, Divor ^a   Baldi, Lucia ^a   Hacker, David L ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

Antibody; Chinese hamster ovary cells; Hypothermia; Orbital shaking; Polyethylenimine; Transient gene expression

Indexed keywords

A-DENSITY; BATCH CULTURE; BIOPROCESSES; CELL VIABILITY; CHINESE HAMSTER OVARY CELLS; CHO CELL; DNA DELIVERY; ORBITAL SHAKING; PLASMID DNA; POLYETHYLENIMINES; PRODUCTION PHASIS; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN; SIMPLIFIED METHOD; SMALL SCALE; TRANSIENT GENE EXPRESSION; TRANSIENT GENE EXPRESSIONS; YIELDING PROCESS;

ANTIBODIES; BATCH CELL CULTURE; CELL CULTURE; CELLS; DRUG THERAPY; HYPOTHERMIA; NUCLEIC ACIDS;

GENE EXPRESSION;

PLASMID DNA; POLYETHYLENEIMINE; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BIOPROCESS; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; DNA TRANSFECTION; FED BATCH CULTURE; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; TRANSIENT EXPRESSION;

ANIMALS; BIOTECHNOLOGY; CELL COUNT; CHO CELLS; CRICETINAE; CRICETULUS; DNA; GENE EXPRESSION; PLASMIDS; POLYETHYLENEIMINE; RECOMBINANT PROTEINS; TRANSFECTION;

CRICETULUS GRISEUS;

EID: 79955536348     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.03.001     Document Type: Article

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