Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells

Methods

Volumn 55, Issue 4, 2011, Pages 273-280

  Chaudhary, Sarika ^a,b   Pak, John E ^a,b   Pedersen, Bjørn P ^a,b   Bang, Lois J ^a,b   Zhang, Liye B ^a,b   Ngaw, Samantha M M ^a,b   Green, Raissa G ^a,b   Sharma, Vinay ^c   Stroud, Robert M ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c BANASTHALI UNIVERSITY   (India)

Author keywords

Detergent screening; FSEC; HEK293; Human membrane proteins; Overexpression; Transient transfection

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN;

ARTICLE; CELL STRAIN HEK293; EMBRYO; FLUORESCENCE; FLUORESCENCE DETECTION SIZE EXCLUSION CHROMATOGRAPHY; GEL PERMEATION CHROMATOGRAPHY; GENE OVEREXPRESSION; GENETIC TRANSFECTION; GLYCOSYLATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SCREENING; TRANSIENT TRANSFECTION; WESTERN BLOTTING;

BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; MEMBRANE PROTEINS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TRANSFECTION;

MAMMALIA;

EID: 84855985970     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2011.08.018     Document Type: Article

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