Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells

Journal of Biotechnology

Volumn 128, Issue 2, 2007, Pages 268-280

  Carpentier, Eric ^a   Paris, Sébastien ^a   Kamen, Amine A ^a   Durocher, Yves ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

HEK293 cells; Polyethylenimine; Protein production; pTT vector; Transient gene expression

Indexed keywords

HEK293 CELLS; POLYETHYLENIMINE; PROTEIN PRODUCTION; PTT VECTORS; TRANSIENT GENE EXPRESSION;

CELLS; DNA; GENE TRANSFER; PARAMETER ESTIMATION; TITRATION;

PROTEINS;

CELL NUCLEUS ANTIGEN; EPSTEIN BARR NUCLEAR ANTIGEN 1; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; PLASMID DNA; POLYETHYLENEIMINE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOTECHNOLOGY; BOTTLENECK POPULATION; CELL POPULATION; CELL SELECTION; CELL STRAIN HEK293; DNA TRANSCRIPTION; DNA TRANSFER; EMBRYO; GENE EXPRESSION; GENE TARGETING; GENE TECHNOLOGY; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONVIRAL GENE DELIVERY SYSTEM; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; RNA SYNTHESIS; STATISTICAL SIGNIFICANCE; TRANSIENT EXPRESSION;

BLOTTING, NORTHERN; CELL LINE; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; KIDNEY; PLASMIDS; POLYETHYLENEIMINE; RECOMBINANT PROTEINS; RNA, MESSENGER; TRANSFECTION; TRANSGENES;

MAMMALIA;

EID: 33846049519     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.10.014     Document Type: Article

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