A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

Methods

Volumn 55, Issue 1, 2011, Pages 44-51

  Raymond, Céline ^a,c   Tom, Roseanne ^b   Perret, Sylvie ^b   Moussouami, Pascal ^a   L'Abbé, Denis ^b   St Laurent, Gilles ^b   Durocher, Yves ^a,b,c  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c Glycoform Monoclonal Antibodies (MabNet)   (Canada)

Author keywords

EBNA1; HEK293; Polyethylenimine; Serum free; Suspension culture; Transfection

Indexed keywords

ALKALINE PHOSPHATASE PLACENTA ISOENZYME; CHIMERIC ANTIBODY; GREEN FLUORESCENT PROTEIN; PLASMID DNA; POLYETHYLENEIMINE; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; CELL DIFFERENTIATION; CELL STRAIN HEK293; EMBRYO; EXPRESSION VECTOR; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; INCUBATION TIME; INTERMETHOD COMPARISON; LARGE SCALE PRODUCTION; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SUSPENSION CELL CULTURE; TRANSIENT EXPRESSION;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; DNA; GENE EXPRESSION; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MAMMALS; NANOPARTICLES; PLASMIDS; POLYETHYLENEIMINE; RECOMBINANT PROTEINS; TRANSFECTION;

MAMMALIA;

EID: 80053647240     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2011.04.002     Document Type: Article

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