Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells

Biotechnology and Bioengineering

Volumn 75, Issue 2, 2001, Pages 197-203

  Meissner, Petra ^a,c   Pick, Horst ^a   Kulangara, Alexandra ^a   Chatellard, Philippe ^b   Friedrich, Kirstin ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

^c MICROMET AG   (Germany)

Author keywords

Calcium phosphate; HEK293 EBNA; Suspension; Transient transfection

Indexed keywords

BIOREACTORS; CALCIUM COMPOUNDS; CELLS; DNA; ESCHERICHIA COLI; MICROSCOPIC EXAMINATION; PROTEINS;

TRANSIENT GENE EXPRESSION (TGE);

GENES;

CALCIUM PHOSPHATE; DNA BINDING PROTEIN; GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN G ANTIBODY; MEMBRANE RECEPTOR; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; CELL LINE; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PLASMID; PROTEIN SYNTHESIS;

BIOREACTORS; BLOTTING, WESTERN; CELL LINE; CULTURE MEDIA; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; ESCHERICHIA COLI; GENE EXPRESSION; GENES, REPORTER; GENETIC MARKERS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOGLOBULIN G; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; PLASMIDS; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT3; RECOMBINANT PROTEINS; TIME FACTORS; TRANSFECTION;

EID: 0035922883     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1179     Document Type: Article

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