Serum-free large-scale transient transfection of CHO cells

Biotechnology and Bioengineering

Volumn 87, Issue 4, 2004, Pages 537-545

  Derouazi, Madiha ^a   Girard, Philippe ^a   Van Tilborgh, Frédéric ^a   Iglesias, Keyvan ^a   Muller, Natalie ^a   Bertschinger, Martin ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

CHO; Polyethylenimine; Transient gene expression

Indexed keywords

CELL DENSITY; OVARIES; SERUM; TRANSIENT GENE EXPRESSION (TGE);

BIOREACTORS; DNA; GENES; OPTIMIZATION; PARAMETER ESTIMATION; PROTEINS; PURIFICATION; THERMAL EFFECTS;

CELLS;

CALCIUM PHOSPHATE; DNA; GREEN FLUORESCENT PROTEIN; POLYETHYLENEIMINE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BIOREACTOR; CHO CELL; CULTURE MEDIUM; DNA CONTENT; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENETIC TRANSFECTION; NONHUMAN; PLASMID; PROTEIN ANALYSIS; SPECTROPHOTOMETRY; TRANSIENT EXPRESSION;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, SERUM-FREE; DNA; DRUG DELIVERY SYSTEMS; POLYETHYLENEIMINE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION; VEHICLES;

CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 4344632530     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20161     Document Type: Article

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