메뉴 건너뛰기




Volumn 899, Issue , 2012, Pages 203-219

Transient expression technologies: Past, present, and future

Author keywords

CHO cells; HEK293 cells; Lipofection; Polyethylenimine; Recombinant protein expression; Transient transfection

Indexed keywords

LIPOPEPTIDE; RECOMBINANT PROTEIN;

EID: 84864123068     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-921-1_13     Document Type: Article
Times cited : (42)

References (94)
  • 1
    • 33845935849 scopus 로고    scopus 로고
    • Largescale transfection of mammalian cells for the fast production of recombinant protein
    • Pham PL, Kamen A, Durocher Y (2006) Largescale transfection of mammalian cells for the fast production of recombinant protein. Mol Biotechnol 34:225-237
    • (2006) Mol. Biotechnol. , vol.34 , pp. 225-237
    • Pham, P.L.1    Kamen, A.2    Durocher, Y.3
  • 2
    • 34147125994 scopus 로고    scopus 로고
    • Recombinant protein production by large-scale transient gene expression in mammalian cells: State of the art and future perspectives
    • Baldi L, Hacker DL, Adam M, Wurm FM (2007) Recombinant protein production by large-scale transient gene expression in mammalian cells: State of the art and future perspectives. Biotechnol Lett 29:677-684
    • (2007) Biotechnol. Lett. , vol.29 , pp. 677-684
    • Baldi, L.1    Hacker, D.L.2    Adam, M.3    Wurm, F.M.4
  • 3
    • 70449710875 scopus 로고    scopus 로고
    • Expression systems for therapeutic glycoprotein production
    • Durocher Y, Butler M (2009) Expression systems for therapeutic glycoprotein production. Curr Opin Biotechnol 20:700-707
    • (2009) Curr. Opin. Biotechnol. , vol.20 , pp. 700-707
    • Durocher, Y.1    Butler, M.2
  • 4
    • 58749101153 scopus 로고    scopus 로고
    • Reflections on more than 10 years of TGE approaches
    • Geisse S (2009) Reflections on more than 10 years of TGE approaches. Protein Expr Purif 64:99-107
    • (2009) Protein Expr. Purif. , vol.64 , pp. 99-107
    • Geisse, S.1
  • 5
    • 80053647240 scopus 로고    scopus 로고
    • A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications
    • Raymond C, Tom R, Perret S, Moussouami P, L'Abbe D, St-Laurent G, Durocher Y (2011) A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications. Methods 55(1):44-51
    • (2011) Methods , vol.55 , Issue.1 , pp. 44-51
    • Raymond, C.1    Tom, R.2    Perret, S.3    Moussouami, P.4    L'Abbe, D.5    St-Laurent, G.6    Durocher, Y.7
  • 6
    • 24344436812 scopus 로고    scopus 로고
    • Largescale transient expression of therapeutic proteins in mammalian cells
    • Smales C, James D (eds Humana Press, Totowa NJ
    • Geisse S, Jordan M, Wurm F (2005) Largescale transient expression of therapeutic proteins in mammalian cells. In: Smales C, James D (eds) Therapeutic proteins. Humana Press, Totowa NJ, pp 87-98
    • (2005) Therapeutic Proteins , pp. 87-98
    • Geisse, S.1    Jordan, M.2    Wurm, F.3
  • 7
    • 71549154680 scopus 로고    scopus 로고
    • Recombinant protein production by transient gene transfer into mammalian cells
    • Geisse S, Fux C (2009) Recombinant protein production by transient gene transfer into mammalian cells. Methods Enzymol 463:223-238
    • (2009) Methods Enzymol , vol.463 , pp. 223-238
    • Geisse, S.1    Fux, C.2
  • 8
    • 0017710978 scopus 로고
    • Characteristics of a human cell line transformed by DNA from human adenovirus type 5
    • Graham FL, Smiley J, Russell WC, Nairn R (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 36:59-72
    • (1977) J. Gen. Virol. , vol.36 , pp. 59-72
    • Graham, F.L.1    Smiley, J.2    Russell, W.C.3    Nairn, R.4
  • 9
    • 37249084096 scopus 로고    scopus 로고
    • Pleiotrophic functions of Epstein-Barr virus nuclear antigen-1 (EBNA-1) and oriP differentially contribute to the ef fi cacy of transfection/ expression of exogenous gene in mammalian cells
    • Kishida T, Asada H, Kubo K, Sato YT, Shin-Ya M, Imanishi J, Yoshikawa K, Mazda O (2008) Pleiotrophic functions of Epstein-Barr virus nuclear antigen-1 (EBNA-1) and oriP differentially contribute to the ef fi cacy of transfection/ expression of exogenous gene in mammalian cells. J Biotechnol 133:201-207
    • (2008) J. Biotechnol. , vol.133 , pp. 201-207
    • Kishida, T.1    Asada, H.2    Kubo, K.3    Sato, Y.T.4    Shin-Ya, M.5    Imanishi, J.6    Yoshikawa, K.7    Mazda, O.8
  • 11
    • 22344434392 scopus 로고    scopus 로고
    • The role of plasmid constructs containing the SV40 DNA nuclear-targeting sequence in cationic lipidmediated DNA delivery
    • Prasad TK, Rao NM (2005) The role of plasmid constructs containing the SV40 DNA nuclear-targeting sequence in cationic lipidmediated DNA delivery. Cell Mol Biol Lett 10:203-215
    • (2005) Cell Mol. Biol. Lett. , vol.10 , pp. 203-215
    • Prasad, T.K.1    Rao, N.M.2
  • 12
    • 70350277265 scopus 로고    scopus 로고
    • Nucleosome assembly proteins bind to Epstein-Barr virus nuclear antigen 1 and affect its functions in DNA replication and transcriptional activation
    • Wang S, Frappier L (2009) Nucleosome assembly proteins bind to Epstein-Barr virus nuclear antigen 1 and affect its functions in DNA replication and transcriptional activation. J Virol 83:11704-11714
    • (2009) J. Virol. , vol.83 , pp. 11704-11714
    • Wang, S.1    Frappier, L.2
  • 13
    • 0013261473 scopus 로고    scopus 로고
    • Establishment of a human somatic hybrid cell line for recombinant protein production
    • Cho MS, Yee H, Chan S (2002) Establishment of a human somatic hybrid cell line for recombinant protein production. J Biomed Sci 9:631-638
    • (2002) J. Biomed. Sci. , vol.9 , pp. 631-638
    • Cho, M.S.1    Yee, H.2    Chan, S.3
  • 14
    • 0035745388 scopus 로고    scopus 로고
    • An oriP expression vector containing the HIV Tat/TAR transactivation axis produces high levels of protein expression in mammalian cells
    • Cho M-S, Yee H, Brown C, Jeang K-T, Cahn S (2001) An oriP expression vector containing the HIV Tat/TAR transactivation axis produces high levels of protein expression in mammalian cells. Cytotechnology 37:23-30
    • (2001) Cytotechnology , vol.37 , pp. 23-30
    • Cho, M.-S.1    Yee, H.2    Brown, C.3    Jeang, K.-T.4    Cahn, S.5
  • 15
    • 46349100443 scopus 로고    scopus 로고
    • Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells
    • Liu C, Dalby B, Chen W, Kilzer JM, Chiou HC (2008) Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells. Mol Biotechnol 39:141-153
    • (2008) Mol. Biotechnol. , vol.39 , pp. 141-153
    • Liu, C.1    Dalby, B.2    Chen, W.3    Kilzer, J.M.4    Chiou, H.C.5
  • 16
    • 0031746208 scopus 로고    scopus 로고
    • Up to 100-fold increase of apparent gene expression in the presence of Epstein-Barr virus oriP sequences and EBNA1: Implications of the nuclear import of plasmids
    • Laengle-Rouault F, Patzel V, Benavente A, Taillez M, Silvestre N, Bompard A, Sczakiel G, Jacobs E, Rittner K (1998) Up to 100-fold increase of apparent gene expression in the presence of Epstein-Barr virus oriP sequences and EBNA1: Implications of the nuclear import of plasmids. J Virol 72:6181-6185
    • (1998) J. Virol. , vol.72 , pp. 6181-6185
    • Laengle-Rouault, F.1    Patzel, V.2    Benavente, A.3    Taillez, M.4    Silvestre, N.5    Bompard, A.6    Sczakiel, G.7    Jacobs, E.8    Rittner, K.9
  • 17
    • 46349094993 scopus 로고    scopus 로고
    • Rational vector design for Efficient non-viral gene delivery: Challenges facing the use of plasmid DNA
    • Mairhofer J, Grabherr R (2008) Rational vector design for Efficient non-viral gene delivery: Challenges facing the use of plasmid DNA. Mol Biotechnol 39:97-104
    • (2008) Mol. Biotechnol. , vol.39 , pp. 97-104
    • Mairhofer, J.1    Grabherr, R.2
  • 18
    • 0037082158 scopus 로고    scopus 로고
    • Highlevel and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
    • Durocher Y, Perret S, Kamen A (2002) Highlevel and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res 30:E9
    • (2002) Nucleic. Acids Res. , vol.30
    • Durocher, Y.1    Perret, S.2    Kamen, A.3
  • 19
    • 0035689186 scopus 로고    scopus 로고
    • Effects of pCIneo and pCEP4 expression vectors on transient and stable protein production in human and simian cell lines
    • Parham J, Kost T, Hutchins J (2001) Effects of pCIneo and pCEP4 expression vectors on transient and stable protein production in human and simian cell lines. Cytotechnology 35:181-187
    • (2001) Cytotechnology , vol.35 , pp. 181-187
    • Parham, J.1    Kost, T.2    Hutchins, J.3
  • 20
    • 17444398577 scopus 로고    scopus 로고
    • Prolonged and increased expression of soluble Fc receptors, IgG and a TCR-Ig fusion protein by transiently transfected adherent 293E cells
    • Berntzen G, Lunde E, Flobakk M, Andersen JT, Lauvrak V, Sandlie I (2005) Prolonged and increased expression of soluble Fc receptors, IgG and a TCR-Ig fusion protein by transiently transfected adherent 293E cells. J Immunol Methods 298:93-104
    • (2005) J. Immunol. Methods , vol.298 , pp. 93-104
    • Berntzen, G.1    Lunde, E.2    Flobakk, M.3    Andersen, J.T.4    Lauvrak, V.5    Sandlie, I.6
  • 21
    • 84864150199 scopus 로고    scopus 로고
    • Patent Application Publication US2011/0039339 A1
    • Durocher Y, Loignon M (2011) US Patent Application Publication US2011/0039339 A1
    • (2011) US
    • Durocher, Y.1    Loignon, M.2
  • 23
    • 79955919279 scopus 로고    scopus 로고
    • Enhanced CHO cell-based transient gene expression with the Epi-CHO expression system
    • Codamo J, Munro TP, Hughes BS, Song M, Gray PP (2011) Enhanced CHO cell-based transient gene expression with the Epi-CHO expression system. Mol Biotechnol 48:109-115
    • (2011) Mol. Biotechnol. , vol.48 , pp. 109-115
    • Codamo, J.1    Munro, T.P.2    Hughes, B.S.3    Song, M.4    Gray, P.P.5
  • 24
    • 50049130165 scopus 로고    scopus 로고
    • Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures
    • Backliwal G, Hildinger M, Kuettel I, Delegrange F, Hacker DL, Wurm FM (2008) Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures. Biotechnol Bioeng 101:182-189
    • (2008) Biotechnol. Bioeng , vol.101 , pp. 182-189
    • Backliwal, G.1    Hildinger, M.2    Kuettel, I.3    Delegrange, F.4    Hacker, D.L.5    Wurm, F.M.6
  • 25
  • 26
    • 56649099058 scopus 로고    scopus 로고
    • Coexpression of acidic fi broblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells
    • Backliwal G, Hildinger M, Chenuet S, de Jesus M, Wurm FM (2008) Coexpression of acidic fi broblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells. N Biotechnol 25: 162-166
    • (2008) N. Biotechnol. , vol.25 , pp. 162-166
    • Backliwal, G.1    Hildinger, M.2    Chenuet, S.3    De Jesus, M.4    Wurm, F.M.5
  • 28
    • 78650204558 scopus 로고    scopus 로고
    • Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting
    • Pichler J, Galosy S, Mott J, Borth N (2011) Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting. Biotechnol Bioeng 108:386-394
    • (2011) Biotechnol. Bioeng. , vol.108 , pp. 386-394
    • Pichler, J.1    Galosy, S.2    Mott, J.3    Borth, N.4
  • 31
    • 33745038944 scopus 로고    scopus 로고
    • Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells
    • Galbraith DJ, Tait AS, Racher AJ, Birch JR, James DC (2006) Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells. Biotechnol Prog 22:753-762
    • (2006) Biotechnol. Prog. , vol.22 , pp. 753-762
    • Galbraith, D.J.1    Tait, A.S.2    Racher, A.J.3    Birch, J.R.4    James, D.C.5
  • 33
    • 37549052183 scopus 로고    scopus 로고
    • High-density transient gene expression in suspension- adapted 293 EBNA1 cells
    • Sun X, Hia HC, Goh PE, Yap MG (2008) High-density transient gene expression in suspension- adapted 293 EBNA1 cells. Biotechnol Bioeng 99:108-116
    • (2008) Biotechnol. Bioeng , vol.99 , pp. 108-116
    • Sun, X.1    Hia, H.C.2    Goh, P.E.3    Yap, M.G.4
  • 35
    • 27944503506 scopus 로고    scopus 로고
    • Dilution of reporter gene with stuffer DNA does not alter the transfection ef fi ciency of polyethylenimines
    • Kichler A, Leborgne C, Danos O (2005) Dilution of reporter gene with stuffer DNA does not alter the transfection ef fi ciency of polyethylenimines. J Gene Med 7:1459-1467
    • (2005) J. Gene Med. , vol.7 , pp. 1459-1467
    • Kichler, A.1    Leborgne, C.2    Danos, O.3
  • 36
    • 38449121649 scopus 로고    scopus 로고
    • High-density transfection with HEK- 293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI
    • Backliwal G, Hildinger M, Hasija V, Wurm FM (2008) High-density transfection with HEK- 293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol Bioeng 99:721-727
    • (2008) Biotechnol. Bioeng , vol.99 , pp. 721-727
    • Backliwal, G.1    Hildinger, M.2    Hasija, V.3    Wurm, F.M.4
  • 37
    • 79955521240 scopus 로고    scopus 로고
    • Transient gene expression in HEK293 and vero cells immobilised on microcarriers
    • Fliedl L, Kaisermayer C (2011) Transient gene expression in HEK293 and vero cells immobilised on microcarriers. J Biotechnol 153:15-21
    • (2011) J. Biotechnol. , vol.153 , pp. 15-21
    • Fliedl, L.1    Kaisermayer, C.2
  • 38
    • 33751242770 scopus 로고    scopus 로고
    • Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethyleniminemediated DNA delivery
    • Bertschinger M, Backliwal G, Schertenleib A, Jordan M, Hacker DL, Wurm FM (2006) Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethyleniminemediated DNA delivery. J Control Release 116:96-104
    • (2006) J. Control Release , vol.116 , pp. 96-104
    • Bertschinger, M.1    Backliwal, G.2    Schertenleib, A.3    Jordan, M.4    Hacker, D.L.5    Wurm, F.M.6
  • 39
    • 56749131191 scopus 로고    scopus 로고
    • The kinetics of polyethylenimine-mediated transfection in suspension culture of Chinese hamster ovary cells
    • Bertschinger M, Schertenleib A, Cevey J, Hacker D, Wurm F (2008) The kinetics of polyethylenimine-mediated transfection in suspension culture of Chinese hamster ovary cells. Mol Biotechnol 40:136-143
    • (2008) Mol. Biotechnol. , vol.40 , pp. 136-143
    • Bertschinger, M.1    Schertenleib, A.2    Cevey, J.3    Hacker, D.4    Wurm, F.5
  • 41
    • 67649100143 scopus 로고    scopus 로고
    • Iron (III) citrate inhibits polyethylenimine-mediated transient transfection of Chinese hamster ovary cells in serum-free medium
    • Eberhardy SR, Radzniak L, Liu Z (2009) Iron (III) citrate inhibits polyethylenimine-mediated transient transfection of Chinese hamster ovary cells in serum-free medium. Cytotechnology 60:1-9
    • (2009) Cytotechnology , vol.60 , pp. 1-9
    • Eberhardy, S.R.1    Radzniak, L.2    Liu, Z.3
  • 42
    • 40149107402 scopus 로고    scopus 로고
    • Development of a generic transient transfection process at 100 L scale
    • Tuvesson O, Uhe C, Rozkov A, Lullau E (2008) Development of a generic transient transfection process at 100 L scale. Cytotechnology 56:123-136
    • (2008) Cytotechnology , vol.56 , pp. 123-136
    • Tuvesson, O.1    Uhe, C.2    Rozkov, A.3    Lullau, E.4
  • 43
    • 14244252530 scopus 로고    scopus 로고
    • Orbital shaker technology for the cultivation of mammalian cells in suspension
    • Muller N, Girard P, Hacker DL, Jordan M, Wurm FM (2005) Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnol Bioeng 89:400-406
    • (2005) Biotechnol. Bioeng , vol.89 , pp. 400-406
    • Muller, N.1    Girard, P.2    Hacker, D.L.3    Jordan, M.4    Wurm, F.M.5
  • 44
    • 79955597234 scopus 로고    scopus 로고
    • Design of experiment in CHO and HEK transient transfection condition optimization
    • Bollin F, Dechavanne V, Chevalet L (2011) Design of experiment in CHO and HEK transient transfection condition optimization. Protein Expr Purif 78:61-68
    • (2011) Protein Expr. Purif. , vol.78 , pp. 61-68
    • Bollin, F.1    Dechavanne, V.2    Chevalet, L.3
  • 45
    • 33845936631 scopus 로고    scopus 로고
    • Serum-free suspension large-scale transient transfection of CHO cells in wave bioreactors
    • Haldankar R, Danqing L, Saremi Z, Baikalov C, Deshpande R (2006) Serum-free suspension large-scale transient transfection of CHO cells in wave bioreactors. Mol Biotechnol 34:191-199
    • (2006) Mol. Biotechnol. , vol.34 , pp. 191-199
    • Haldankar, R.1    Danqing, L.2    Saremi, Z.3    Baikalov, C.4    Deshpande, R.5
  • 46
    • 33745284997 scopus 로고    scopus 로고
    • Natural polymers for gene delivery and tissue engineering
    • Dang JM, Leong KW (2006) Natural polymers for gene delivery and tissue engineering. Adv Drug Deliv Rev 58:487-499
    • (2006) Adv. Drug Deliv. Rev. , vol.58 , pp. 487-499
    • Dang, J.M.1    Leong, K.W.2
  • 48
    • 33751103699 scopus 로고    scopus 로고
    • Gene transfer effects on various cationic amphiphiles in CHO cells
    • Kusumoto K, Akao T, Mizuki E, Nakamura O (2006) Gene transfer effects on various cationic amphiphiles in CHO cells. Cytotechnology 51:57-66
    • (2006) Cytotechnology , vol.51 , pp. 57-66
    • Kusumoto, K.1    Akao, T.2    Mizuki, E.3    Nakamura, O.4
  • 49
    • 37148999397 scopus 로고    scopus 로고
    • Novel orbital shake bioreactors for transient production of CHO derived IgGs
    • Stettler M, Zhang X, Hacker DL, Md J, Wurm FM (2007) Novel orbital shake bioreactors for transient production of CHO derived IgGs. Biotechnol Prog 23:1340-1346
    • (2007) Biotechnol. Prog. , vol.23 , pp. 1340-1346
    • Stettler, M.1    Zhang, X.2    Hacker, D.L.3    Md, J.4    Wurm, F.M.5
  • 53
    • 77951837717 scopus 로고    scopus 로고
    • Evaluating regulatory elements of human cytomegalovirus major immediate early gene for enhancing transgene expression levels in CHO K1 and HEK293 cells
    • Mariati Ng YK, Chao SH, Yap MG, Yang Y (2010) Evaluating regulatory elements of human cytomegalovirus major immediate early gene for enhancing transgene expression levels in CHO K1 and HEK293 cells. J Biotechnol 147:160-163
    • (2010) J. Biotechnol. , vol.147 , pp. 160-163
    • Mariati Ng, Y.K.1    Chao, S.H.2    Yap, M.G.3    Yang, Y.4
  • 54
    • 70350574634 scopus 로고    scopus 로고
    • Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells
    • Mariati Ho SC, Yap MG, Yang Y (2010) Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells. Protein Expr Purif 69:9-15
    • (2010) Protein Expr. Purif. , vol.69 , pp. 9-15
    • Mariati Ho, S.C.1    Yap, M.G.2    Yang, Y.3
  • 55
    • 33646051851 scopus 로고    scopus 로고
    • WPREmediated enhancement of gene expression is promoter and cell line specific
    • Klein R, Ruttkowski B, Knapp E, Salmons B, Gunzburg WH, Hohenadl C (2006) WPREmediated enhancement of gene expression is promoter and cell line specific. Gene 372: 153-161
    • (2006) Gene , vol.372 , pp. 153-161
    • Klein, R.1    Ruttkowski, B.2    Knapp, E.3    Salmons, B.4    Gunzburg, W.H.5    Hohenadl, C.6
  • 57
    • 50849109798 scopus 로고    scopus 로고
    • Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
    • Backliwal G, Hildinger M, Chenuet S, Wulhfard S, De Jesus M, Wurm FM (2008) Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Res 36:e96
    • (2008) Nucleic. Acids Res. , vol.36
    • Backliwal, G.1    Hildinger, M.2    Chenuet, S.3    Wulhfard, S.4    De Jesus, M.5    Wurm, F.M.6
  • 58
    • 33644500399 scopus 로고    scopus 로고
    • Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells
    • Kwaks TH, Otte AP (2006) Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells. Trends Biotechnol 24:137-142
    • (2006) Trends Biotechnol. , vol.24 , pp. 137-142
    • Kwaks, T.H.1    Otte, A.P.2
  • 59
    • 79957471146 scopus 로고    scopus 로고
    • Identi fi cation of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections
    • Harraghy N, Regamey A, Girod PA, Mermod N (2011) Identi fi cation of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections. J Biotechnol 154:11-20
    • (2011) J. Biotechnol. , vol.154 , pp. 11-20
    • Harraghy, N.1    Regamey, A.2    Girod, P.A.3    Mermod, N.4
  • 61
    • 77954458920 scopus 로고    scopus 로고
    • Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
    • Wulhfard S, Baldi L, Hacker DL, Wurm F (2010) Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. J Biotechnol 148:128-132
    • (2010) J. Biotechnol. , vol.148 , pp. 128-132
    • Wulhfard, S.1    Baldi, L.2    Hacker, D.L.3    Wurm, F.4
  • 62
    • 42549114735 scopus 로고    scopus 로고
    • Sodium butyrate stimulates monoclonal antibody over-Expression in CHO cells by improving gene accessibility
    • Jiang Z, Sharfstein ST (2008) Sodium butyrate stimulates monoclonal antibody over-Expression in CHO cells by improving gene accessibility. Biotechnol Bioeng 100:189-194
    • (2008) Biotechnol. Bioeng , vol.100 , pp. 189-194
    • Jiang, Z.1    Sharfstein, S.T.2
  • 63
    • 41249093488 scopus 로고    scopus 로고
    • Genomic and proteomic exploration of CHO and hybridoma cells under sodium butyrate treatment
    • Yee JC, de Leon Gatti M, Philp RJ, Yap M, Hu WS (2008) Genomic and proteomic exploration of CHO and hybridoma cells under sodium butyrate treatment. Biotechnol Bioeng 99:1186-1204
    • (2008) Biotechnol. Bioeng , vol.99 , pp. 1186-1204
    • Yee, J.C.1    De Leon Gatti, M.2    Philp, R.J.3    Yap, M.4    Hu, W.S.5
  • 65
    • 57349087717 scopus 로고    scopus 로고
    • Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells
    • Hwang SO, Lee GM (2009) Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells. J Biotechnol 139:89-94
    • (2009) J. Biotechnol. , vol.139 , pp. 89-94
    • Hwang, S.O.1    Lee, G.M.2
  • 66
    • 33846707942 scopus 로고    scopus 로고
    • Valproic acid and butyrate induce apoptosis in human cancer cells through inhibition of gene expression of Akt/protein kinase B
    • Chen J, Ghazawi FM, Bakkar W, Li Q (2006) Valproic acid and butyrate induce apoptosis in human cancer cells through inhibition of gene expression of Akt/protein kinase B. Mol Cancer 5:71
    • (2006) Mol. Cancer , vol.5 , pp. 71
    • Chen, J.1    Ghazawi, F.M.2    Bakkar, W.3    Li, Q.4
  • 68
    • 27144492957 scopus 로고    scopus 로고
    • Direct regulation of rRNA transcription by fi broblast growth factor 2
    • Sheng Z, Liang Y, Lin CY, Comai L, Chirico WJ (2005) Direct regulation of rRNA transcription by fi broblast growth factor 2. Mol Cell Biol 25:9419-9426
    • (2005) Mol. Cell Biol. , vol.25 , pp. 9419-9426
    • Sheng, Z.1    Liang, Y.2    Lin, C.Y.3    Comai, L.4    Chirico, W.J.5
  • 69
    • 20544460820 scopus 로고    scopus 로고
    • Active hypothermic growth: A novel means for increasing total interferon-gamma production by Chinese-hamster ovary cells
    • Fox SR, Yap MX, Yap MG, Wang DI (2005) Active hypothermic growth: A novel means for increasing total interferon-gamma production by Chinese-hamster ovary cells. Biotechnol Appl Biochem 41:265-272
    • (2005) Biotechnol. Appl. Biochem. , vol.41 , pp. 265-272
    • Fox, S.R.1    Yap, M.X.2    Yap, M.G.3    Wang, D.I.4
  • 71
    • 70350141643 scopus 로고    scopus 로고
    • Enhanced interferon-beta production by CHO cells through elevated osmolality and reduced culture temperature
    • Han YK, Koo TY, Lee GM (2009) Enhanced interferon-beta production by CHO cells through elevated osmolality and reduced culture temperature. Biotechnol Prog 25:1440-1447
    • (2009) Biotechnol. Prog. , vol.25 , pp. 1440-1447
    • Han, Y.K.1    Koo, T.Y.2    Lee, G.M.3
  • 73
    • 33745259524 scopus 로고    scopus 로고
    • Enhancement of transient gene expression by fed-batch culture of HEK 293 EBNA1 cells in suspension
    • Sun X, Goh PE, Wong KT, Mori T, Yap MG (2006) Enhancement of transient gene expression by fed-batch culture of HEK 293 EBNA1 cells in suspension. Biotechnol Lett 28:843-848
    • (2006) Biotechnol. Lett. , vol.28 , pp. 843-848
    • Sun, X.1    Goh, P.E.2    Wong, K.T.3    Mori, T.4    Yap, M.G.5
  • 75
    • 70350491149 scopus 로고    scopus 로고
    • Degradation of an Fc-fusion recombinant protein by host cell proteases: Identi fi cation of a CHO cathepsin D protease
    • Robert F, Bierau H, Rossi M, Agugiaro D, Soranzo T, Broly H, Mitchell-Logean C (2009) Degradation of an Fc-fusion recombinant protein by host cell proteases: Identi fi cation of a CHO cathepsin D protease. Biotechnol Bioeng 104:1132-1141
    • (2009) Biotechnol. Bioeng , vol.104 , pp. 1132-1141
    • Robert, F.1    Bierau, H.2    Rossi, M.3    Agugiaro, D.4    Soranzo, T.5    Broly, H.6    Mitchell-Logean, C.7
  • 77
    • 13544259722 scopus 로고    scopus 로고
    • On the optimal ratio of heavy to light chain genes for Efficient recombinant antibody production by CHO cells
    • Schlatter S, Stans fi eld SH, Dinnis DM, Racher AJ, Birch JR, James DC (2005) On the optimal ratio of heavy to light chain genes for Efficient recombinant antibody production by CHO cells. Biotechnol Prog 21:122-133
    • (2005) Biotechnol. Prog. , vol.21 , pp. 122-133
    • Schlatter, S.1    Stansfield, S.H.2    Dinnis, D.M.3    Racher, A.J.4    Birch, J.R.5    James, D.C.6
  • 78
    • 0032412131 scopus 로고    scopus 로고
    • Differential ef fi ciency of expression of humanized antibodies in transient transfected mammalian cells
    • Bentley KJ, Gewert R, Harris WJ (1998) Differential ef fi ciency of expression of humanized antibodies in transient transfected mammalian cells. Hybridoma 17:559-567
    • (1998) Hybridoma , vol.17 , pp. 559-567
    • Bentley, K.J.1    Gewert, R.2    Harris, W.J.3
  • 80
    • 67650167709 scopus 로고    scopus 로고
    • Two routes for production and puri fi cation of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells
    • Zhao Y, Gutshall L, Jiang H, Baker A, Beil E, Obmolova G, Carton J, Taudte S, Amegadzie B (2009) Two routes for production and puri fi cation of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells. Protein Expr Purif 67:182-189
    • (2009) Protein Expr. Purif. , vol.67 , pp. 182-189
    • Zhao, Y.1    Gutshall, L.2    Jiang, H.3    Baker, A.4    Beil, E.5    Obmolova, G.6    Carton, J.7    Taudte, S.8    Amegadzie, B.9
  • 82
    • 0032970591 scopus 로고    scopus 로고
    • Analysis of expression kinetics and activity of a new B-domain truncated and fulllength FVIII protein in three different cell lines
    • Haack A, Schmitt C, Poller W, Oldenburg J, Han fl and P, Brackmann HH, Schwaab R (1999) Analysis of expression kinetics and activity of a new B-domain truncated and fulllength FVIII protein in three different cell lines. Ann Hematol 78:111-116
    • (1999) Ann. Hematol. , vol.78 , pp. 111-116
    • Haack, A.1    Schmitt, C.2    Poller, W.3    Oldenburg, J.4    Hanfland, P.5    Brackmann, H.H.6    Schwaab, R.7
  • 83
    • 38349095577 scopus 로고    scopus 로고
    • Puri fi cation of the extracellular domain of the membrane protein glialcam expressed in hek and cho cells and comparison of the glycosylation
    • Gaudry JP, Arod C, Sauvage C, Busso S, Dupraz P, Pankiewicz R, Antonsson B (2008) Puri fi cation of the extracellular domain of the membrane protein GlialCAM expressed in HEK and CHO cells and comparison of the glycosylation. Protein Expr Purif 58:94-102
    • (2008) Protein Expr. Purif. , vol.58 , pp. 94-102
    • Gaudry, J.P.1    Arod, C.2    Sauvage, C.3    Busso, S.4    Dupraz, P.5    Pankiewicz, R.6    Antonsson, B.7
  • 88
    • 79956205237 scopus 로고    scopus 로고
    • Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic fl ux analysis
    • Niklas J, Schrader E, Sandig V, Noll T, Heinzle E (2011) Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic fl ux analysis. Bioprocess Biosyst Eng 34: 533-545
    • (2011) Bioprocess Biosyst Eng. , vol.34 , pp. 533-545
    • Niklas, J.1    Schrader, E.2    Sandig, V.3    Noll, T.4    Heinzle, E.5
  • 89
    • 40449122158 scopus 로고    scopus 로고
    • Efficient and reproducible generation of high-Expressing, stable human cell lines without need for antibiotic selection
    • Schiedner G, Hertel S, Bialek C, Kewes H, Waschutza G, Volpers C (2008) Efficient and reproducible generation of high-Expressing, stable human cell lines without need for antibiotic selection. BMC Biotechnol 8:13
    • (2008) BMC Biotechnol. , vol.8 , pp. 13
    • Schiedner, G.1    Hertel, S.2    Bialek, C.3    Kewes, H.4    Waschutza, G.5    Volpers, C.6
  • 90
    • 78149319046 scopus 로고    scopus 로고
    • The avian EB66(R) cell line, application to vaccines, and therapeutic protein production
    • Brown SW, Mehtali M (2010) The avian EB66(R) cell line, application to vaccines, and therapeutic protein production. PDA J Pharm Sci Technol 64:419-425
    • (2010) PDA J. Pharm. Sci. Technol. , vol.64 , pp. 419-425
    • Brown, S.W.1    Mehtali, M.2
  • 91
    • 77956230926 scopus 로고    scopus 로고
    • Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches
    • Kramer O, Klausing S, Noll T (2010) Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches. Appl Microbiol Biotechnol 88:425-436
    • (2010) Appl. Microbiol. Biotechnol. , vol.88 , pp. 425-436
    • Kramer, O.1    Klausing, S.2    Noll, T.3
  • 94
    • 78650509694 scopus 로고    scopus 로고
    • MicroRNAs: Tiny targets for engineering CHO cell phenotypes
    • Barron N, Sanchez N, Kelly P, Clynes M (2011) MicroRNAs: Tiny targets for engineering CHO cell phenotypes? Biotechnol Lett 33:11-21
    • (2011) Biotechnol. Lett. , vol.33 , pp. 11-21
    • Barron, N.1    Sanchez, N.2    Kelly, P.3    Clynes, M.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.