Purification and characterization of a recombinant G-protein-coupled receptor, Saccharomyces cerevisiae Ste2p, transiently expressed in HEK293 EBNA1 cells

Biochemistry

Volumn 44, Issue 48, 2005, Pages 15705-15714

  Shi, Chunhua ^a   Shin, Youn Ok ^a,b,c   Hanson, John ^a   Cass, Brian ^b   Loewen, Michele C ^a   Durocher, Yves ^b  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL ORGANS; CELL CULTURE; FLUORESCENCE; HYDROPHOBICITY; MOLECULAR STRUCTURE; PURIFICATION;

EXTRACELLULAR LOOPS; FLUORESCENCE MICROSCOPY; LIGANDS; MILLIGRAMS;

PROTEINS;

5 MALEIMIDOFLUORESCEIN; 7 (DIETHYLAMINO) 3 (4' MALEIMIDYLPHENYL) 4 METHYLCOUMARIN; AMINE; BENZENE DERIVATIVE; COUMARIN DERIVATIVE; CYSTEINE DERIVATIVE; FC RECEPTOR; FLUORESCENT DYE; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; HISTIDINE; HYBRID PROTEIN; LIGAND; MALEIMIDE DERIVATIVE; MATING HORMONE ALPHA FACTOR; MEMBRANE PROTEIN; METAL; PHEROMONE; PROTEIN A; PROTEIN STE2P; RECOMBINANT RECEPTOR; SEPHAROSE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELL STRAIN HEK293; FLUORESCENCE MICROSCOPY; FUNGUS CULTURE; HUMAN; HUMAN CELL; HYDROPHILICITY; HYDROPHOBICITY; LIGAND BINDING; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE;

CELLS, CULTURED; HUMANS; PEPTIDES; PHEROMONES; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, MATING FACTOR; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSFECTION;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 28544450037     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051292p     Document Type: Article

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