Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

Biotechnology Letters

Volumn 34, Issue 4, 2012, Pages 619-626

  Rajendra, Yashas ^a   Kiseljak, Divor ^a   Baldi, Lucia ^a   Hacker, David L ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

Ammonia; CHO DG44; Glutamine; HEK 293E; Stable gene expression; Transient gene expression

Indexed keywords

CELL LINES; CHO CELL; CHO-DG44; CYTOTOXIC; GLUTAMINE; GROWTH ARREST; HEK-293E; INITIAL CONCENTRATION; MAMMALIAN CELLS; MEDIUM COMPONENTS; POLYCLONAL; PROTEIN PRODUCTION; RECOMBINANT PROTEIN PRODUCTIONS; TRANSIENT GENE EXPRESSION;

AMMONIA; ANIMAL CELL CULTURE; GENE EXPRESSION; RECOMBINANT PROTEINS;

AMINO ACIDS;

AMMONIA; GLUTAMINE; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL STRAIN HEK293; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE MEDIUM; CULTURE TECHNIQUE; HAMSTER; HUMAN; METABOLISM; METHODOLOGY;

AMMONIA; ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLUTAMINE; HEK293 CELLS; HUMANS; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 84858008748     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0809-z     Document Type: Article

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