Partial enzymatic deglycosylation preserves the structure of cleaved recombinant HIV-1 envelope glycoprotein trimers

Journal of Biological Chemistry

Volumn 287, Issue 29, 2012, Pages 24239-24254

  Depetris, Rafael S ^a   Julien, Jean Philippe ^b   Khayat, Reza ^b   Lee, Jeong Hyun ^b   Pejchal, Robert ^b   Katpally, Umesh ^c   Cocco, Nicolette ^a   Kachare, Milind ^a   Massi, Evan ^a   David, Kathryn B ^a   Cupo, Albert ^a   Marozsan, Andre J ^a   Olson, William C ^c   Ward, Andrew B ^b   Wilson, Ian A ^b   Sanders, Rogier W ^a,d   Moore, John P ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c Clinical Research   (United States)

^d UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; DISEASES; EPITOPES; GLYCOPROTEINS; POLYSACCHARIDES; VIRUSES;

CONFORMATIONAL EPITOPES; ENVELOPE GLYCOPROTEINS; HUMAN IMMUNODEFICIENCY VIRUS TYPE-1; MICROSCOPIC ANALYSIS; NEUTRALIZING ANTIBODIES; PROTEOLYTIC DIGESTION; STRUCTURAL HETEROGENEITY; VACCINE DEVELOPMENT;

OLIGOMERS;

CD209 ANTIGEN; CD4 ANTIGEN; ENVELOPE PROTEIN; EPITOPE; GLYCAN DERIVATIVE; GLYCOPROTEIN GP 120; GLYCOPROTEIN GP 140; GLYCOPROTEIN GP 41; GLYCOSIDASE; PROTEIN ANTIBODY; RECOMBINANT PROTEIN;

ARTICLE; BINDING SITE; CARBOHYDRATE SYNTHESIS; CELL STRAIN HEK293; CONTROLLED STUDY; DEGLYCOSYLATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; INHIBITION KINETICS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN STRUCTURE; WESTERN BLOTTING;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84863788697     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.371898     Document Type: Article

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