Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors

Biochemical Engineering Journal

Volumn 52, Issue 2-3, 2010, Pages 181-186

  Tissot, Stéphanie ^a   Farhat, Mohamed ^a   Hacker, David L ^a   Anderlei, Tibor ^b   Kühner, Markus ^b   Comninellis, Christos ^a   Wurm, Florian ^a  

^a EPFL   (Switzerland)

^b ADOLF KÜHNER AG   (Switzerland)

Author keywords

Free surface; Mixing; Mixing time; Orbital shaking; Scale up; Shaken bioreactor

Indexed keywords

FREE SURFACES; MIXING TIME; ORBITAL SHAKING; SCALE-UP; SHAKEN BIOREACTOR;

BIOCONVERSION; BIOREACTORS; CELL CULTURE; CONTAINERS; LIQUIDS; MAMMALS;

MIXING;

AGITATION; ARTICLE; BIOREACTOR; COLORIMETRY; EQUIPMENT; GEOMETRY; IMAGE ANALYSIS; PH; PRIORITY JOURNAL; REACTION TIME; SCALE UP;

MAMMALIA;

EID: 77957969022     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2010.08.005     Document Type: Article

References (26)

1. Liu C.-M., Hong L.-N. Development of a shaking bioreactor system for animal cell cultures. Biochem. Eng. J. 2001, 7(2):121-125.
2. Kumar S., Wittmann C., Heinzle E. Minibioreactors. Biotechnol. Lett. 2004, 26(1):1-10.
3. Muller N., Girard P., Hacker D.L., Jordan M., Wurm F.M. Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnol. Bioeng. 2005, 89(4):400-406.
4. De Jesus M.J., Girard P., Bourgeois M., Baumgartner G., Jacko B., Amstutz H., Wurm F.M. Tubespin satellites: a fast track approach for process development with animal cells using shaking technology. Biochem. Eng. J. 2004, 17(3):217-223.
5. Büchs J. Introduction to advantages and problems of shaken cultures. Biochem. Eng. J. 2001, 7(2):91-98.
6. M. Stettler, Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells, Ph.D. thesis, EPFL, 2007.
7. Zhang X., Stettler M., Sanctis D., Perrone M., Parolini N., Discacciati M., Jesus M.D., Hacker D., Quarteroni A., Wurm F. Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1000-liter scale. Adv. Biochem. Eng. Biotechnol. 2009, 1-21.
8. Micheletti M., Barrett T., Doig S.D., Baganz F., Levy M.S., Woodley J.M., Lye G.J. Fluid mixing in shaken bioreactors: implications for scale-up predictions from microlitre-scale microbial and mammalian cell cultures. Chem. Eng. Sci. 2006, 61(9):2939-2949.
9. Zlokarnik M. Stirring. Ullmann's Encyclopedia of Industrial Chemistry, Electronic Release 2003, Wiley-VCH, Weinheim, Germany. 7th edition.
10. Kaeppel M. Development and application of a method for measuring the mixture quality of miscible liquids. 1. State of research and theoretical principles. Int. Chem. Eng. 1979, 19(2):196-215.
11. Hiby J.W. Definition and measurement of the degree of mixing in liquid mixtures. Int. Chem. Eng. 1981, 21(2):197-204.
12. Kasat G.R., Pandit A.B. Mixing time studies in multiple impeller agitated reactors. Can. J. Chem. Eng. 2004, 82(5):892-904.
13. Melton L.A., Lipp C.W., Spradling R.W., Paulson K.A. DISMT-determination of mixing time through color changes. Chem. Eng. Commun. 2002, 189(3):322-338.
14. Koynov A., Tryggvason G., Khinast J.G. Characterization of the localized hydrodynamic shear forces and dissolved oxygen distribution in sparged bioreactors. Biotechnol. Bioeng. 2007, 97(2):317-331.
15. Cabaret F., Bonnot S., Fradette L., Tanguy P.A. Mixing time analysis using colorimetric methods and image processing. Ind. Eng. Chem. Res. 2007, 46(14):5032-5042.
16. Ju L.-K., Chase G. Improved scale-up strategies of bioreactors. Bioprocess. Eng. 1992, 8:49-53.
17. Junker B.H. Scale-up methodologies for Escherichia coli and yeast fermentation processes. J. Biosci. Bioeng. 2004, 97(6):347-364.
18. Xing Z., Kenty B.M., Li Z.J., Lee S.S. Scale-up analysis for a CHO cell culture process in large-scale bioreactors. Biotechnol. Bioeng. 2009, 103(4):733-746.
19. Lara A.R., Galindo E., Ramrez O.T., Palomares L.A. Living with heterogeneities in bioreactors: understanding the effects of environmental gradients on cells. Mol. Biotechnol. 2006, 34(3):355-381.
20. Mithani R., Shenoi S., Fan L.T., Walawender W.P. Ranking dimensionless groups in fluidized-bed reactor scale-up. Int. J. Approx. Reason. 1990, 4(1):69-85.
21. Verma M., Brar S.K., Sreekrishnan T.R., Tyagi R.D., Surampalli R.Y. Dimensionless groups as scale-up parameter for wastewater and wastewater sludge treatment in a stirred tank reactor. J. Residuals Sci. Technol. 2007, 4(1):35-43.
22. Yamaguchi H. Engineering Fluid Mechanics 2008, Springer, Dordrecht, The Netherlands.
23. Rushton J. Applications of fluid mechanics and similitude to scale-up problems. Part 1. Chem. Eng. Prog. 1952, 48:33-38.
24. Fisher R.A. Statistical Methods for Research Workers 1925, Oliver & Boyd, Edinburgh.
25. Bonvillani P., Ferrari M.P., Ducrós E.M., Orejas J.A. Theoretical and experimental study of the effects of scale-up on mixing time for a stirred-tank bioreactor. Braz. J. Chem. Eng. 2006, 23(1):1-7.
26. Langheinrich C., Nienow A.W. Control of pH in large-scale, free suspension animal cell bioreactors: alkali addition and pH excursions. Biotechnol. Bioeng. 1999, 66(3):171-179.