Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 28, 2011, Pages 11440-11445

  Harris, Audray ^a   Borgnia, Mario J ^a   Shi, Dan ^a   Bartesaghi, Alberto ^a   He, Haifeng ^b   Pejchal, Robert ^c   Kang, Yun ^d   Depetris, Rafael ^e   Marozsan, Andre J ^d   Sanders, Rogier W ^e   Klasse, Per Johan ^e   Milne, Jacqueline L S ^a   Wilson, Ian A ^c   Olson, William C ^d   Moore, John P ^e   Subramaniam, Sriram ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b Thermo Fisher Scientific   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

^d A Subsidiary of Lantheus Holdings   (United States)

^e WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

AIDS vaccine; Cryoelectron microscopy; HIV spike; HIV vaccine; Viral entry

Indexed keywords

CD4 ANTIGEN; GLYCOPROTEIN GP 120; GLYCOPROTEIN GP 140; HIV 1 ENVELOPE GLYCOPROTEIN; TRIMERIC HIV 1 GLYCOPROTEIN GP140; UNCLASSIFIED DRUG; VIRUS ENVELOPE PROTEIN;

ARTICLE; AVERAGING; BINDING SITE; CONTROLLED STUDY; ELECTRON TOMOGRAPHY; HUMAN IMMUNODEFICIENCY VIRUS 1; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN CONFORMATION; PROTEIN QUATERNARY STRUCTURE; SOLUBILIZATION; VIRION;

AIDS VACCINES; ANTIGENS, CD4; BIOPHYSICAL PHENOMENA; CRYOELECTRON MICROSCOPY; ENV GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; HIV ANTIBODIES; HIV ANTIGENS; HIV ENVELOPE PROTEIN GP120; HIV-1; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; LIGANDS; MODELS, MOLECULAR; PROTEIN STRUCTURE, QUATERNARY;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 79960974710     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1101414108     Document Type: Article

References (30)

1. Gallo SA, et al. (2003) The HIV Env-mediated fusion reaction. Biochim Biophys Acta 1614:36-50. (Pubitemid 36857094)
2. Liu J, Bartesaghi A, Borgnia MJ, Sapiro G, Subramaniam S (2008) Molecular architecture of native HIV-1 gp120 trimers. Nature 455:109-113.
3. White TA, et al. (2010) Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: Strain-dependent variation in quaternary structure. PLoS Pathog 6:e1001249.
4. Huang CC, et al. (2005) Structure of a V3-containing HIV-1 gp120 core. Science 310:1025-1028.
5. Kwong PD, et al. (1998) Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. Nature 393:648-659. (Pubitemid 28289647)
6. Zhou T, et al. (2007) Structural definition of a conserved neutralization epitope on HIV-1 gp120. Nature 445:732-737. (Pubitemid 46279718)
7. Pancera M, et al. (2010) Structure of HIV-1 gp120 with gp41-interactive region reveals layered envelope architecture and basis of conformational mobility. Proc Natl Acad Sci USA 107:1166-1171.
8. Walker LM, Burton DR Rational antibody-based HIV-1 vaccine design: Current approaches and future directions. Curr Opin Immunol 22:358-366.
9. Mascola JR, Montefiori DC The role of antibodies in HIV vaccines. Annu Rev Immunol 28:413-444.
10. Pantophlet R, Burton DR (2006) GP120: Target for neutralizing HIV-1 antibodies. Annu Rev Immunol 24:739-769.
11. Beddows S, et al. (2006) Construction and characterization of soluble, cleaved, and stabilized trimeric Env proteins based on HIV type 1 Env subtype A. AIDS Res Hum Retroviruses 22:569-579.
12. Kang YK, et al. (2009) Structural and immunogenicity studies of a cleaved, stabilized envelope trimer derived from subtype A HIV-1. Vaccine 27:5120-5132.
13. Lee JE, et al. (2008) Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor. Nature 454:177-182. (Pubitemid 351969896)
14. Binley JM, et al. (2000) A recombinant human immunodeficiency virus type 1 envelope glycoprotein complex stabilized by an intermolecular disulfide bond between the gp120 and gp41 subunits is an antigenic mimic of the trimeric virion-associated structure. J Virol 74:627-643. (Pubitemid 30036198)
15. Moore JP, Trkola A, Sattentau QJ, Cao Y, Ho DD (1994) Studies on the interactions of monoclonal antibodies with HIV-1 isolates from clades A-F. Neuvieme Colloque Des Cent Gardes 151-155.
16. Wu SR, et al. (2010) Single-particle cryoelectron microscopy analysis reveals the HIV-1 spike as a tripod structure. Proc Natl Acad Sci USA 107:18844-18849.
17. Moscoso CG, et al. (2011) Quaternary structures of HIV Env immunogen exhibit conformational vicissitudes and interface diminution elicited by ligand binding. Proc Natl Acad Sci USA 108:6091-6096.
18. Hoffman TL, et al. (1999) Stable exposure of the coreceptor-binding site in a CD4-independent HIV-1 envelope protein. Proc Natl Acad Sci USA 96:6359-6364. (Pubitemid 29256670)
19. Beddows S, et al. (2007) A comparative immunogenicity study in rabbits of disulfidestabilized, proteolytically cleaved, soluble trimeric human immunodeficiency virus type 1 gp140, trimeric cleavage-defective gp140 and monomeric gp120. Virology 360:329-340. (Pubitemid 46482615)
20. Yang X, Wyatt R, Sodroski J (2001) Improved elicitation of neutralizing antibodies against primary human immunodeficiency viruses by soluble stabilized envelope glycoprotein trimers. J Virol 75:1165-1171. (Pubitemid 32094675)
21. Bower JF, Yang X, Sodroski J, Ross TM (2004) Elicitation of neutralizing antibodies with DNA vaccines expressing soluble stabilized human immunodeficiency virus type 1 envelope glycoprotein trimers conjugated to C3d. J Virol 78:4710-4719. (Pubitemid 38501092)
22. Klasse P, Sanders R, Cerutti A, Moore J (2011) How can HIV-type-1 Env immunogenicity be improved to facilitate antibody-based vaccine development? AIDS Res Hum Retroviruses, 10.1089/aid.2011.0053.
23. Burton DR, et al. (1994) Efficient neutralization of primary isolates of HIV-1 by a recombinant human monoclonal antibody. Science 266:1024-1027. (Pubitemid 24373715)
24. Walker LM, et al. (2009) Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target. Science 326:285-289.
25. Wu X, et al. (2010) Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1. Science 329:856-861.
26. Corti D, et al. (2010) Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PLoS One 5:e8805.
27. Bartesaghi A, Subramaniam S (2009) Membrane protein structure determination using cryo-electron tomography and 3D image averaging. Curr Opin Struct Biol 19:402-407.
28. Bartesaghi A, et al. (2008) Classification and 3D averaging with missing wedge correction in biological electron tomography. J Struct Biol 162:436-450.
29. Sanders RW, et al. (2002) Stabilization of the soluble, cleaved, trimeric form of the envelope glycoprotein complex of human immunodeficiency virus type 1. J Virol 76:8875-8889. (Pubitemid 34864082)
30. Beddows S, et al. (2005) Evaluating the immunogenicity of a disulfide-stabilized, cleaved, trimeric form of the envelope glycoprotein complex of human immunodeficiency virus type 1. J Virol 79:8812-8827. (Pubitemid 40934792)