Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency

Biotechnology and Bioengineering

Volumn 84, Issue 3, 2003, Pages 332-342

  Pham, Phuong Lan ^a   Perret, Sylvie ^a   Doan, Huyen Chau ^a   Cass, Brian ^a   St Laurent, Gilles ^a   Kamen, Amine ^a   Durocher, Yves ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Bioreactor; Human embryonic kidney cells; Immobilized metal affinity chromatography; Polyethyleneimine; Secreted proteins

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOREACTORS; CELLS; FLUORESCENCE; PROTEINS;

TRANSIENT TRANSFECTION;

BIOTECHNOLOGY;

ALKALINE PHOSPHATASE; GREEN FLUORESCENT PROTEIN; NEUROPILIN 1; PEPTONE; PROTEIN HYDROLYSATE; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; CELL GROWTH; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SECRETION; REPORTER GENE; VOLUMETRY;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL LINE; CELL SURVIVAL; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; HUMANS; KIDNEY; NEUROPILIN-1; PEPTONES; PROTEIN ENGINEERING; RECEPTOR, TIE-2; RECOMBINANT PROTEINS; TRANSFECTION;

MAMMALIA; VECTORS;

EID: 0141867826     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10774     Document Type: Article

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