Protein digestion: An overview of the available techniques and recent developments

Journal of Proteome Research

Volumn 12, Issue 3, 2013, Pages 1067-1077

  Switzar, Linda ^a   Giera, Martin ^a,b   Niessen, Wilfried M A ^a,c  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c Hyphen MassSpec   (Netherlands)

Author keywords

accelerated digestion; bottom up proteomics; chemical digestion; enzymatic digestion; IMER; membrane proteins; protein digestion; PTM

Indexed keywords

ACETIC ACID; AMMONIA; ARGININE; CHYMOTRYPSIN; CYANOGEN BROMIDE; CYSTEINE; DISULFIDE; DITHIOTHREITOL; FORMIC ACID; GUANIDINE; HYDROCHLORIC ACID; HYDROXYLAMINE; IMMOBILIZED ENZYME; IMMUNOGLOBULIN G; LYSINE; MEMBRANE PROTEIN; METALLOPROTEINASE; METHANOL; PAPAIN; PEPSIN A; PRONASE; PROTEIN; PROTEINASE; THERMOLYSIN; TRYPSIN;

ALKYLATION; CATALYSIS; COLLISIONALLY ACTIVATED DISSOCIATION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME DEGRADATION; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; HUMAN; LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROWAVE IRRADIATION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEOMICS; REDUCTION; REVIEW; SAMPLING; SEQUENCE ANALYSIS; STREPTOCOCCUS PYOGENES; TANDEM MASS SPECTROMETRY; TEMPERATURE; THERMOSTABILITY; ULTRASOUND;

EID: 84874616206     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr301201x     Document Type: Review

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