Multidigestion in continuous flow tandem protease-immobilized microreactors for proteomic analysis

Analytical Biochemistry

Volumn 407, Issue 1, 2010, Pages 12-18

  Yamaguchi, Hiroshi ^a   Miyazaki, Masaya ^a,b   Kawazumi, Hirofumi ^c   Maeda, Hideaki ^a,b,d  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c KINKI UNIVERSITY   (Japan)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Enzyme immobilization; Microreactor; Multidigestion; Posttranslational modification; Protease; Proteolysis

Indexed keywords

ENZYME IMMOBILIZATION; MASS SPECTROMETRY; PHOSPHATASES; PROTEOLYSIS;

ALKALINE PHOSPHATASE; IN-SOLUTION DIGESTIONS; MICRO-REACTOR; MULTIDIGESTION; PHOSPHORYLATION SITES; POST-TRANSLATIONAL MODIFICATIONS; PROTEASE; TIME-CONSUMING PROCEDURE;

CHEMICAL REACTORS;

ALKALINE PHOSPHATASE; BETA CASEIN; CHYMOTRYPSIN; CYTOCHROME C; PEPSIN A; PROTEINASE; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CONTINUOUS FLOW REACTOR; CONTROLLED STUDY; FEASIBILITY STUDY; ISOTOPE LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; PROTEOMICS;

EID: 77956877523     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.07.026     Document Type: Article

References (24)

1. Hunter T. Tyrosine phosphorylation: thirty years and counting. Curr. Opin. Cell Biol. 2009, 21:140-146.
2. Domon B., Aebersold R. Mass spectrometry and protein analysis. Science 2006, 312:212-217.
3. Witze E.S., Old W.N., Resing K.A., Ahn N.G. Mapping protein post-translational modifications with mass spectrometry. Nat. Methods 2007, 10:798-806.
4. Choudhary G., Wu S.-L., Shieh P., Hancock W.S. Multiple enzymatic digestion for enhanced sequence coverage of proteins in complex proteomic mixtures using capillary LC with ion trap MS/MS. J. Proteome Res. 2003, 2:59-67.
5. Fischer F., Poetsch A. Protein cleavage strategies for an improved analysis of the membrane proteome. Proteome Sci. 2006, 4:2.
6. Han G., Ye M., Jiang X., Chen R., Ren J., Xue Y., Wang F., Song C., Yao X., Zou H. Comprehensive and reliable phosphorylation site mapping of individual phosphoproteins by combination of multiple stage mass spectrometric analysis with a target-decoy database search. Anal. Chem. 2009, 81:5794-5805.
7. Kinoshita E., Kinoshita-Kikuta E., Takiyama K., Koike T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol. Cell. Proteomics 2006, 5:749-757.
8. Zhao Y., Jensen O.N. Modification-specific proteomics: strategies for characterization of post-translational modifications using enrichment techniques. Proteomics 2009, 9:4632-4641.
9. Ma J., Zhang L., Liang Z., Zhang W., Zhang Y. Recent advance in immobilized enzymatic reactors and their applications in proteome analysis. Anal. Chim. Acta 2009, 632:1-8.
10. Miyazaki M., Maeda H. Microchannel enzyme reactors and their applications for processing. Trends Biotechnol. 2006, 24:463-470.
11. Miyazaki M., Honda T., Yamaguchi H., Briones M.P.P., Maeda H. Biotechnology and Genetic Engineering Reviews 2008, vol. 25:405-428. Nottingham University Press, Nottingham, UK.
12. Dulay M.T., Baca Q.J., Zare R.N. Enhanced proteolytic activity of covalently bound enzymes in photopolymerized sol-gel. Anal. Chem. 2005, 77:4604-4610.
13. Honda T., Miyazaki M., Nakamura H., Maeda H. Facile preparation of an enzyme-immobilized microreactor using a cross-linking enzyme membrane on a microchannel surface. Adv. Synth. Catal. 2006, 348:2163-2171.
14. Yamaguchi H., Miyazaki M., Honda T., Briones-Nagata M.P., Arima K., Maeda H. Rapid and efficient proteolysis for proteomic analysis by protease-immobilized microreactor. Electrophoresis 2009, 30:3257-3264.
15. Ma J., Zhang L., Liang Z., Zhang W., Zhang Y. Monolith-based immobilized enzyme reactors: recent developments and applications for proteome analysis. J. Sep. Sci. 2007, 30:3050-3059.
16. Honda T., Miyazaki M., Nakamura H., Maeda H. Immobilization of enzymes on a microchannel surface through cross-linking polymerization. Chem. Commun. 2005, 5062-5064.
17. Temporini C., Calleri E., Cabrera K., Felix G., Massolini G. On-line multi-enzymatic approach for improved sequence coverage in protein analysis. J. Sep. Sci. 2009, 32:1120-1128.
18. Sakai-Kato K., Kato M., Toyooka T. On-line trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis. Anal. Chem. 2002, 74:2943-2949.
19. Li Y., Xu X., Deng C., Yang P., Zhang X. Immobilization of trypsin on superparamagnetic nanoparticles for rapid and effective proteolysis. J. Proteome Res. 2007, 6:3849-3855.
20. Liu T., Bao H., Zhang L., Chen G. Integration of electrodes in a suction cup-driven microchip for alternating current-accelerated proteolysis. Electrophoresis 2009, 30:3265-3268.
21. 2 core-shell microspheres for selective enrichment of phosphopeptides in phosphoproteome analysis. J. Proteome Res. 2007, 7:2526-2538.
22. Krokhin O.V., Antonovici M., Ens W., Wilkins J.A., Standing K.G. Deamidation of - Asn-Gly - sequences during sample preparation for proteomics: consequence for MALDI and HPLC-MALDI analysis. Anal. Chem. 2006, 78:6645-6650.
23. Bongers J., Heimer E.P., Lambros T., Pan Y.C., Campbell R.M., Felix A.M. Degradation of aspartic acid and asparagine residues in human growth hormone-releasing factor. Int. J. Pept. Protein Res. 1992, 39:364-374.
24. Dick L.W., Kim C., Qiu D., Cheng K.C. Determination of the origin of the N-terminal pyro-glutamate variation in monoclonal antibodies using model peptides. Biotechnol. Bioeng. 2007, 97:544-553.