Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: A new approach to protein digestion in minutes

Protein Science

Volumn 11, Issue 11, 2002, Pages 2676-2687

  Pramanik, Birendra N ^a   Mirza, Urooj A ^b   Ing, Yao Hain ^a   Liu, Yan Hui ^a   Bartner, Peter L ^a   Weber, Patricia C ^a   Bose, Ajay K ^a,b  

^a MERCK RESEARCH LABORATORIES   (United States)

^b Stevens Institute of Technology   (United States)

Author keywords

Accelerated protein digestion; Mass spectrometry; Microwave irradiation; Selective endoprotease reactions

Indexed keywords

ALPHA2B INTERFERON; CYTOCHROME C; LYSINE; LYSOZYME; MYOGLOBIN; PEPTIDE; PROTEINASE; TRYPSIN; UBIQUITIN; PEPTIDE FRAGMENT;

ARTICLE; CHEMICAL REACTION KINETICS; DIGESTION; ENZYMATIC DEGRADATION; ENZYME MECHANISM; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MICROWAVE RADIATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; ANIMAL; CATTLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; PEPTIDE MAPPING; TIME;

ANIMALS; CATTLE; CYTOCHROME C GROUP; INTERFERON ALFA-2B; MICROWAVES; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS; TRYPSIN; UBIQUITIN;

EID: 0036838857     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0213702     Document Type: Article

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