Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics

Journal of Proteomics

Volumn 75, Issue 4, 2012, Pages 1454-1462

  Burkhart, Julia Maria ^a   Schumbrutzki, Cornelia ^a   Wortelkamp, Stefanie ^a   Sickmann, Albert ^a,b   Zahedi, René Peiman ^a  

^a LEIBNIZ INSTITUT FÜR ANALYTISCHE WISSENSCHAFTEN ISAS E V   (Germany)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Digest control; Digestion; Quality control; Quantitative proteomics; SCX; Trypsin

Indexed keywords

CHYMOTRYPSIN; PROTEINASE; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEOMICS; QUALITATIVE ANALYSIS; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; TANDEM MASS SPECTROMETRY; THROMBOCYTE;

BLOOD PLATELETS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, LIQUID; HUMANS; MASS SPECTROMETRY; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; TRYPSIN;

EID: 84855877960     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2011.11.016     Document Type: Article

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