Optimization of digestion parameters for protein quantification

Analytical Biochemistry

Volumn 393, Issue 1, 2009, Pages 48-55

  Norrgran, Jessica ^a   Williams, Tracie L ^a   Woolfitt, Adrian R ^a   Solano, Maria I ^a   Pirkle, James L ^a   Barr, John R ^a  

^a NATIONAL CENTER FOR ENVIRONMENTAL HEALTH   (United States)

Author keywords

In solution enzymatic digestion; Liquid chromatography tandem mass spectrometry (LC MS MS); Protein quantification

Indexed keywords

ENZYMES; MASS SPECTROMETRY; PEPTIDES;

ENZYMATIC DIGESTIONS; INCUBATION TEMPERATURES; ISOTOPICALLY LABELED; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC MS/MS); LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; MULTIPLE-REACTION MONITORING MODES; PEPTIDE STANDARDS; PROTEIN QUANTIFICATION;

LIQUID CHROMATOGRAPHY;

PEPTIDE; RICIN; SURFACTANT; TRYPSIN; VIRUS HEMAGGLUTININ;

ALKYLATION; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME MECHANISM; INCUBATION TEMPERATURE; INCUBATION TIME; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR MODEL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN DEGRADATION; RICINUS COMMUNIS; TANDEM MASS SPECTROMETRY;

RICINUS COMMUNIS;

EID: 67650982761     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.05.050     Document Type: Article

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