Effects of temperature on ultrasound-assisted tryptic protein digestion

Analytical Biochemistry

Volumn 414, Issue 1, 2011, Pages 125-130

  Shin, Seongjae ^a   Yang, Hyo Jik ^a   Kim, Jinhee ^a   Kim, Jeongkwon ^a  

^a Chungnam National University   (South Korea)

Author keywords

Cytochrome c; Mass spectrometry; Matrix assisted laser desorption ionization; Myoglobin; Trypsin; Ultrasonic assisted

Indexed keywords

DESORPTION; DRUG PRODUCTS; MAMMALS; MASS SPECTROMETRY; MATRIX ALGEBRA; PEPTIDES; ULTRASONICS;

BOVINE SERUM ALBUMINS; CYTOCHROME C; EFFECTS OF TEMPERATURE; INCREASING TEMPERATURES; MATRIX ASSISTED LASER DESORPTION/IONIZATION; MYOGLOBIN; TIME OF FLIGHT MASS SPECTROMETRY; TRYPSIN;

TEMPERATURE;

BOVINE SERUM ALBUMIN; CYTOCHROME C; MYOGLOBIN; TRYPSIN;

ARTICLE; CONTROLLED STUDY; ENZYME DEGRADATION; FEMALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; ROOM TEMPERATURE; TEMPERATURE SENSITIVITY; ULTRASOUND;

BOVINAE;

EID: 79955685371     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.02.026     Document Type: Article

References (18)

1. B.N. Pramanik, U.A. Mirza, Y.H. Ing, Y.H. Liu, P.L. Bartner, P.C. Weber, A.K. Bose, Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: a new approach to protein digestion in minutes, Protein Sci. 11 (2002) 2676-2687. (Pubitemid 35191141)
2. D. Lopez-Ferrer, K. Petritis, K.K. Hixson, T.H. Heibeck, R.J. Moore, M.E. Belov, D.G. Camp 2nd, R.D. Smith, Application of pressurized solvents for ultrafast trypsin hydrolysis in proteomics: proteomics on the fly, J. Proteome Res. 7 (2008) 3276-3281.
3. D. Lopez-Ferrer, J.L. Capelo, J. Vazquez, Ultra fast trypsin digestion of proteins by high intensity focused ultrasound, J. Proteome Res. 4 (2005) 1569-1574. (Pubitemid 41464782)
4. G. Vale, R. Rial-Otero, A. Mota, L. Fonseca, J.L. Capelo, Ultrasonic-assisted enzymatic digestion (USAED) for total elemental determination and elemental speciation: a tutorial, Talanta 75 (2008) 872-884.
5. H.M. Santos, C. Mota, C. Lodeiro, I. Moura, I. Isaac, J.L. Capelo, An improved clean sonoreactor-based method for protein identification by mass spectrometry-based techniques, Talanta 77 (2008) 870-875.
6. H.M. Santos, J.L. Capelo, Trends in ultrasonic-based equipment for analytical sample treatment, Talanta 73 (2007) 795-802. (Pubitemid 47588351)
7. K.S. Suslick, Sonochemistry, Science 247 (1990) 1439-1445. (Pubitemid 120007653)
8. F.M. Cordeiro, R.J. Carreira, R. Rial-Otero, M.G. Rivas, I. Moura, J.L. Capelo, Simplifying sample handling for protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Rapid Commun. Mass Spectrom. 21 (2007) 3269-3278.
9. R. Rial-Otero, R.J. Carreira, F.M. Cordeiro, A.J. Moro, H.M. Santos, G. Vale, I. Moura, J.L. Capelo, Ultrasonic assisted protein enzymatic digestion for fast protein identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Sonoreactor versus ultrasonic probe, J. Chromatogr. A 1166 (2007) 101-107. (Pubitemid 47361165)
10. M. Galesio, D.V. Vieira, R. Riall-Otero, C. Lodeiro, I. Moura, J.L. Capelo, Influence of the protein staining in the fast ultrasonic sample treatment for protein identification through peptide mass fingerprint and matrix-assisted laser desorption ionization time of flight mass spectrometry, J. Proteome Res. 7 (2008) 2097-2106.
11. R. Batra, M.N. Gupta, Enhancement of enzyme-activity in aqueous-organic solvent mixtures, Biotechnol. Lett. 16 (1994) 1059-1064.
12. E.J. Finehout, J.R. Cantor, K.H. Lee, Kinetic characterization of sequencing grade modified trypsin, Proteomics 5 (2005) 2319-2321. (Pubitemid 40835400)
13. J. Havlis, H. Thomas, M. Sebela, A. Shevchenko, Fast-response proteomics by accelerated in-gel digestion of proteins, Anal. Chem. 75 (2003) 1300-1306.
14. R.J. Carreira, R. Rial-Otero, D. Lopez-Ferrer, C. Lodeiro, J.L. Capelo, Ultrasonic energy as a new tool for fast isotopic 18O labeling of proteins for mass spectrometry-based techniques: preliminary results, Talanta 76 (2008) 400-406.
15. H.M. Santos, R. Rial-Otero, L. Fernandes, G. Vale, M.G. Rivas, I. Moura, J.L. Capelo, Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, J. Proteome Res. 6 (2007) 3393-3399. (Pubitemid 47522728)
16. B.A. Garcia, N. Siuti, C.E. Thomas, C.A. Mizzen, N.L. Kelleher, Characterization of neurohistone variants and post-translational modifications by electron capture dissociation mass spectrometry, Int. J. Mass Spectrom. 259 (2007) 184-196.
17. S.L. Wu, J. Kim, W.S. Hancock, B. Karger, Extended range proteomic analysis (ERPA): a new and sensitive LC-MS platform for high sequence coverage of complex proteins with extensive post-translational modifications- comprehensive analysis of beta-casein and epidermal growth factor receptor (EGFR), J. Proteome Res. 4 (2005) 1155-1170. (Pubitemid 41208674)
18. P. Xu, J.M. Peng, Characterization of polyubiquitin chain structure by middle-down mass spectrometry, Anal. Chem. 80 (2008) 3438-3444.